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Forage degradability, rumen bacterial adherence and fibrolytic enzyme activity in vitro: effect of pH or glucose concentration

Published online by Cambridge University Press:  29 July 2013

R. FARENZENA ,
G. V. KOZLOSKI ,
M. P. MEZZOMO  and
A. C. FLUCK
R. FARENZENA
Affiliation:
Departamento de Zootecnia (Animal Science), Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
G. V. KOZLOSKI*
Affiliation:
Departamento de Zootecnia (Animal Science), Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
M. P. MEZZOMO
Affiliation:
Departamento de Zootecnia (Animal Science), Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
A. C. FLUCK
Affiliation:
Departamento de Zootecnia (Animal Science), Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
*
*To whom all correspondence should be addressed. Email: kozloski@smail.ufsm.br
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Summary

A set of independent assays were conducted to assess the effects of either pH or glucose concentration on forage degradation, bacterial adherence and on fibrolytic enzyme activity in vitro. For measuring degradation and bacterial adherence, ryegrass (Lolium multiflorum) and bermudagrass (Cynodon dactylon) samples were incubated in vitro for 24 h in the medium at different pH (5·5, 6·0, 6·5 or 7·0) or with different initial glucose concentrations (0, 1000, 2000 and 3000 mg/l). For fibrolytic enzyme activity evaluation, forage samples were incubated in situ and the extracted enzymes were incubated in vitro under the different pH and glucose treatment conditions. The amount of bacteria adhering to samples and the degradability of dry matter (DM) and neutral detergent fibre (NDF) were higher for ryegrass than for bermudagrass, were not affected by glucose concentration and were linearly and positively affected by increased pH. On average, carboxymethylcellulase (CMCase) and xylanase activities were higher for ryegrass than for bermudagrass in the pH assay, whereas the differences between forages were not significant in the glucose assay. For both forage species, the quadratic effect of pH or glucose concentration on CMCase and xylanase activities was significant. Maximum activity of both enzymes was observed at pH 6·0 or at glucose concentration of 2000 mg/l. In conclusion, forage degradation was affected negatively by decreased ruminal pH due to reduced bacterial adherence. In turn, the pH or glucose effect on fibrolytic enzyme activity was not related to their effects on bacterial adherence or forage degradation, indicating that forage degradation is more dependent on the degree of microbial colonization than on the specific activity of bacterial enzymes.

Type
Animal Research Papers
Information
The Journal of Agricultural Science , Volume 152 , Issue 2 , April 2014 , pp. 325 - 332
Copyright
Copyright © Cambridge University Press 2013 

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