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Effects of nitrogen application and supplemental irrigation on canopy temperature and photosynthetic characteristics in winter wheat

  • D. Q. Yang (a1), W. H. Dong (a1), Y. L. Luo (a1) (a2), W. T. Song (a1), T. Cai (a3), Y. Li (a1), Y. P. Yin (a1) and Z. L. Wang (a1)...


Nitrogen (N) application and irrigation to winter wheat may decrease leaf temperature and enhance photosynthesis: as a result, more photosynthates will be allocated to the grains, resulting in higher grain yields. To investigate this hypothesis, a 2-year field study was conducted with three levels of N fertilizer application (no fertilizer, N0; 240 kg N/ha, N1; 360 kg N/ha, N2) and two different water regimes (rainfed with no irrigation, R; irrigation at the over-wintering, stem elongation and grain filling stages, W). The results show that both N application and supplemental irrigation significantly increased grain yield with increases in both grain number/m2 and the 1000-grain weight, viz., WN2>WN1>WN0>RN2>RN1>RN0. In addition, application of N under both water regimes significantly increased flag leaf area, above-ground biomass and single stem productivity and decreased leaf temperature, which led to an increase in net photosynthesis rates and ribulose bisphosphate (RuBP) carboxylase activity. Moreover, analysis of the chlorophyll α fluorescence transient showed that N fertilizer application and supplemental irrigation significantly increased electron donor and acceptor performance of the photosystem II reaction centre.


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Author for correspondence: Z. L. Wang, E-mail:


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Albrizio, R, Todorovic, M, Matic, T and Stellacci, AM (2010) Comparing the interactive effects of water and nitrogen on durum wheat and barley grown in a Mediterranean environment. Field Crops Research 115, 179190.
Balota, M, Payne, WA, Evett, SR and Lazar, MD (2007) Canopy temperature depression sampling to assess grain yield and genotypic differentiation in winter wheat. Crop Science 47, 15181529.
Balota, M, Payne, WA, Evett, SR and Peters, TR (2008) Morphological and physiological traits associated with canopy temperature depression in three closely related wheat lines. Crop Science 48, 18971910.
Barnabás, B, Jäger, K and Fehér, A (2008) The effect of drought and heat stress on reproductive processes in cereals. Plant, Cell and Environment 31, 1138.
Bowne, JB, Erwin, TA, Juttner, J, Schnurbusch, T, Langridge, P, Bacic, A and Roessner, U (2012) Drought responses of leaf tissues from wheat cultivars of differing drought tolerance at the metabolite level. Molecular Plant 5, 418429.
Bürling, K, Cerovic, ZG, Cornic, G, Ducruet, JM, Noga, G and Hunsche, M (2013) Fluorescence-based sensing of drought-induced stress in the vegetative phase of four contrasting wheat genotypes. Environmental and Experimental Botany 89, 5159.
Dahal, K, Knowles, VL, Plaxton, WC and Huner, NPA (2014) Enhancement of photosynthetic performance, water use efficiency and grain yield during long-term growth under elevated CO2 in wheat and rye is growth temperature and cultivar dependent. Environmental and Experimental Botany 106, 207220.
Denčić, S, Kastori, R, Kobiljski, B and Duggan, B (2000) Evaluation of grain yield and its components in wheat cultivars and landraces under near optimal and drought conditions. Euphytica 113, 4352.
Deng, ZY, Zhang, Q, Xu, JF, Chen, M, Qin, SJ and Zhang, SY (2009) Comparative studies of the harm characteristic of hot-dry wind and high temperature heat waves. Advances in Earth Science 24, 865873.
Duggan, BL, Domitruk, DR and Fowler, DB (2000) Yield component variation in winter wheat grown under drought stress. Canadian Journal of Plant Science 80, 739745.
Elbashier, EME, Tahir, ISA, Saad, ASI and Ibrahim, MAS (2012) Wheat genotypic variability in utilizing nitrogen fertilizer for a cooler canopy under a heat-stressed irrigated environment. African Journal of Agricultural Research 7, 385392.
Elliott, J, Deryng, D, Muller, C, Frieler, K, Konzmann, M, Gerten, D, Glotter, M, Flörke, M, Wada, Y, Best, N, Eisner, S, Fekete, BM, Folberth, C, Foster, I, Gosling, SN, Haddeland, I, Khabarov, N, Ludwig, F, Masaki, Y, Olin, S, Rosenzweig, C, Ruane, AC, Satoh, Y, Schmid, E, Stacke, T, Tang, QH and Wisser, D. (2014) Constraints and potentials of future irrigation water availability on agricultural production under climate change. Proceedings of The National Academy of Sciences of the United States of America 111, 32393244.
Falster, DS and Westoby, M (2003) Leaf size and angle vary widely across species: what consequences for light interception? New Phytologist 158, 509525.
Fan, MS, Shen, JB, Yuan, LX, Jiang, RF, Chen, XP, Davies, WJ and Zhang, FS (2012) Improving crop productivity and resource use efficiency to ensure food security and environmental quality in China. Journal of Experimental Botany 63, 1324.
Fang, QX, Ma, L, Green, TR, Yu, Q, Wang, TD and Ahuja, LR (2010) Water resources and water use efficiency in the North China Plain: current status and agronomic management options. Agricultural Water Management 97, 11021116.
Feng, B, Liu, P, Li, G, Dong, ST, Wang, FH, Kong, LA and Zhang, JW (2014) Effect of heat stress on the photosynthetic characteristics in flag leaves at the grain-filling stage of different heat-resistant winter wheat varieties. Journal of Agronomy and Crop Science 200, 143155.
Ferrante, A, Savin, R and Slafer, GA (2013) Floret development and grain setting differences between modern durum wheats under contrasting nitrogen availability. Journal of Experimental Botany 64, 169184.
Guo, ZJ, Shi, Y, Yu, ZW and Zhang, YL (2015) Supplemental irrigation affected flag leaves senescence post-anthesis and grain yield of winter wheat in the Huang-Huai-Hai Plain of China. Field Crops Research 180, 100109.
Hu, LX, Wang, ZL and Huang, BR (2010) Diffusion limitations and metabolic factors associated with inhibition and recovery of photosynthesis from drought stress in a C3 perennial grass species. Physiologia Plantarum 139, 93106.
Jeuffroy, MH and Bouchard, C (1999) Intensity and duration of nitrogen deficiency on wheat grain number. Crop Science 39, 13851393.
Kalaji, HM, Jajoo, A, Oukarroum, A, Brestic, M, Zivcak, M, Samborska, IA and Cetner, MD, Łukasik, I, Goltsev, V and Ladle, RJ (2016) Chlorophyll a, fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions. Acta Physiologiae Plantarum 38, 102. doi: 10.1007/s11738-016-2113-y
Lawlor, DW and Tezara, W (2009) Causes of decreased photosynthetic rate and metabolic capacity in water-deficient leaf cells: a critical evaluation of mechanisms and integration of processes. Annals of Botany 103, 561579.
Leuning, R, Kelliher, FM, De Pury, DGG and Schulze, ED (1995) Leaf nitrogen, photosynthesis, conductance and transpiration: scaling from leaves to canopies. Plant, Cell and Environment 18, 11831200.
Ling, QH, Huang, WH and Jarvis, P (2011) Use a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana . Photosynthesis Research 107, 209214.
Liu, B, Liu, LL, Tian, LY, Cao, WX, Zhu, Y and Asseng, S (2014) Post-heading heat stress and yield impact in winter wheat of China. Global Change Biology 20, 372381.
Lu, CH and Fan, L (2013) Winter wheat yield potentials and yield gaps in the North China Plain. Field Crops Research 143, 98105.
Masoni, A, Ercoli, L, Mariotti, M and Arduini, I (2007) Post-anthesis accumulation and remobilization of dry matter, nitrogen and phosphorus in durum wheat as affected by soil type. European Journal of Agronomy 26, 179186.
Mon, J, Bronson, KF, Hunsaker, DJ, Thorp, KR, White, JW and French, AN (2016) Interactive effects of nitrogen fertilization and irrigation on grain yield, canopy temperature, and nitrogen use efficiency in overhead sprinkler-irrigated durum wheat. Field Crops Research 191, 5465.
Mueller, ND, Gerber, JS, Johnston, M, Ray, DK, Ramankutty, N and Foley, JA (2012) Closing yield gaps through nutrient and water management. Nature 490, 254257.
Nielsen, DC and Halvorson, AD (1990) Nitrogen fertility influence on water stress and yield of winter wheat. Agronomy Journal 83, 10651070.
Parry, MAJ, Reynolds, M, Salvucci, ME, Raines, C, Andralojc, PJ, Zhu, XG, Price, GD, Condon, AG and Furbank, RT (2011) Raising yield potential of wheat. II. Increasing photosynthetic capacity and efficiency. Journal of Experimental Botany 62, 453467.
Pradhan, GP, Xue, QW, Jessup, KE, Rudd, JC, Liu, SY, Devkota, RN and Mahan, JR (2014) Cooler canopy contributes to higher yield and drought tolerance in new wheat cultivars. Crop Science 54, 22752284.
Prasad, PVV and Djanaguiraman, M (2014) Response of floret fertility and individual grain weight of wheat to high temperature stress: sensitive stages and thresholds for temperature and duration. Functional Plant Biology 41, 12611269.
Rajala, A, Hakala, K, Mäkelä, P, Muurinen, S and Peltonen-Sainio, P (2009) Spring wheat response to timing of water deficit through sink and grain filling capacity. Field Crops Research 114, 263271.
Rebetzke, GJ, Rattey, AR, Farquhar, GD, Richards, RA and Condon, AG (2012) Genomic regions for canopy temperature and their genetic association with stomatal conductance and grain yield in wheat. Functional Plant Biology 40, 1433.
Shah, NH and Paulsen, GM (2003) Interaction of drought and high temperature on photosynthesis and grain-filling of wheat. Plant and Soil 257, 219226.
Stone, PJ and Nicolas, ME (1998) The effect of duration of heat stress during grain filling on two wheat varieties differing in heat tolerance: grain growth and fractional protein accumulation. Australian Journal of Plant Physiology 25, 1320.
Strasser, BJ (1997) Donor side capacity of Photosystem II probed by chlorophyll a fluorescence transients. Photosynthesis Research 52, 147155.
Subrahmanyam, D, Subash, N, Haris, A and Sikka, AK (2006) Influence of water stress on leaf photosynthetic characteristics in wheat cultivars differing in their susceptibility to drought. Photosynthetica 44, 125129.
Trost, B, Ellmer, F, Baumecker, M, Meyer-Aurich, A, Prochnow, A and Drastig, K (2014) Effects of irrigation and nitrogen fertilizer on yield, carbon inputs from above-ground harvest residues and soil organic carbon contents of a sandy soil in Germany. Soil Use and Management 30, 209218.
Wang, D, Xu, XX, Zhang, HB, Lin, X and Zhao, Y (2015) Effects of irrigation with micro-sprinkling hoses on canopy temperature and humidity at filling stage and grain weight of wheat. Acta Agronomica Sinica 41, 15641574.
Wang, YW, Xu, C, Lv, CF, Wu, M, Cai, XJ, Liu, ZT, Song, XM, Chen, GX and Lv, CG (2016) Chlorophyll a, fluorescence analysis of high-yield rice (Oryza sativa L.) LYPJ during leaf senescence. Photosynthetica 54, 422429.
Wang, YZ, Zhang, XY, Liu, XW, Zhang, XY, Shao, LW, Sun, HY and Chen, SY (2013) The effects of nitrogen supply and water regime on instantaneous WUE, time-integrated WUE and carbon isotope discrimination in winter wheat. Field Crops Research 144, 236244.
Wei, AL, Zhang, YH, Huang, Q and Wang, ZM (2007) Dynamic characteristics of photosynthetic rate and carbon assimilation enzyme activities of different green organs in different genotypes of wheat. Acta Agronomica Sinica 33, 14261431.
Wheeler, TR, Hong, TD, Ellis, RH, Batts, GR, Morison, JIL and Hadley, P (1996) The duration and rate of grain growth, and harvest index, of wheat (Triticum aestivum L.) in response to temperature and CO2 . Journal of Experimental Botany 47, 623630.
Yang, JC and Zhang, JH (2006) Grain filling of cereals under soil drying. New Phytologist 169, 223236.
Yang, W, Yin, Y, Jiang, W, Peng, D, Yang, D, Cui, Y and Wang, Z (2014) Severe water deficit-induced ethylene production decreases photosynthesis and photochemical efficiency in flag leaves of wheat. Photosynthetica 52, 341350.
Zadoks, JC, Chang, TT and Konzak, CF (1974) A decimal code for the growth stages of cereals. Weed Research 14, 415421.
Zhang, XY, Pei, D, Chen, SY, Sun, HY and Yang, YH (2006) Performance of double-cropped winter wheat–summer maize under minimum irrigation in the north China plain. Agronomy Journal 98, 16201626.
Zhao, DL, Reddy, KR, Kakani, VG and Reddy, VR (2005) Nitrogen deficiency effects on plant growth, leaf photosynthesis, and hyperspectral reflectance properties of sorghum. European Journal of Agronomy 22, 391403.
Zhao, H, Dai, TB, Jing, Q, Jiang, D and Cao, WX (2007) Leaf senescence and grain filling affected by post-anthesis high temperatures in two different wheat cultivars. Plant Growth Regulation 51, 149158.

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Effects of nitrogen application and supplemental irrigation on canopy temperature and photosynthetic characteristics in winter wheat

  • D. Q. Yang (a1), W. H. Dong (a1), Y. L. Luo (a1) (a2), W. T. Song (a1), T. Cai (a3), Y. Li (a1), Y. P. Yin (a1) and Z. L. Wang (a1)...


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