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Effects of N top-dressing modes of panicle fertilization on soil enzymes activity and yield of rice (Oryza sativa L.)

  • Juan Liu (a1) (a2), Jing Zhang (a1) (a2), Xiaodong Chen (a1) (a2), Yanxiu Du (a1) (a2), Junzhou Li (a1) (a2), Hongzheng Sun (a1) (a2), Ting Peng (a1) (a2), Zeyu Xin (a1) (a2) and Quanzhi Zhao (a1) (a2)...


A field study to optimize the nitrogen (N) top-dressing mode of panicle fertilization and improve rice yields was conducted in mid-eastern China. Japonica cultivar Yunongjing-6 was grown and panicle N fertilizer was applied at the beginning of the inverted fourth leaf stage using three different modes: manual broadcast application (BA) on the soil surface as a control treatment, deep application during ditching at a depth of 15 cm (DD) and manual BA on the soil surface during deep ditching (BAD). The activity of soil enzymes, including invertase, urease, phosphatase and catalase, was increased significantly at the jointing, booting and grain-filling stages with the DD and BAD treatments compared with the traditional BA mode. The DD and BAD treatments also increased basal internode and neck-panicle internode bleeding intensity. The DD treatment gave the highest crop yield, increasing the yield by 0.63 and 0.31 t/hm2 in 2011 and 2012, respectively, compared with BA. The results suggest that ditching during panicle N fertilizer application after sun-drying of the fields increases rice yields, most likely by improving the activity of soil enzymes and enhancing the physiological activity of roots and grain weight.


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Author for correspondence: Jing Zhang, E-mail:; Zeyu Xin, E-mail:; Quanzhi Zhao, E-mail:


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Allison, SD, Nielsen, C and Hughes, RF (2006) Elevated enzyme activities in soils under the invasive nitrogen-fixing tree Falcataria moluccana. Soil Biology and Biochemistry 38, 15371544.
Bacon, PE and Lewin, LG (1990) Rice growth under different stubble and nitrogen-fertilization management techniques. Field Crops Research 24, 5165.
Bandaogo, A, Bidjokazo, F, Youl, S, Safo, E, Abaidoo, R and Andrews, O (2015) Effect of fertilizer deep placement with urea supergranule on nitrogen use efficiency of irrigated rice in Sourou Valley (Burkina Faso). Nutrient Cycling in Agroecosystems 102, 7989.
Bandick, AK and Dick, RP (1999) Field management effects on soil enzyme activities. Soil Biology and Biochemistry 31, 14711479.
Belder, P, Bouman, BAM, Cabangon, R, Guoan, L, Quilang, EJP, Yuanhua, L, Spiertz, JHJ and Tuong, TP (2004) Effect of water-saving irrigation on rice yield and water use in typical lowland conditions in Asia. Agricultural Water Management 65, 193210.
Chancy, HF and Kamprath, EJ (1982) Effects of deep tillage on N response by corn on a sandy coastal plain soil. Agronomy Journal 74, 657662.
Cookson, P and Lepiece, AG (1996) Urease enzyme activities in soils of the Batinah region of the Sultanate of Oman. Journal of Arid Environments 32, 225238.
Dick, RP (1994) Soil enzyme activities as indicators of soil quality. In Doran, JW, Coleman, DC, Bezdicek, DF and Stewart, BA (eds), Defining Soil Quality for A Sustainable Environment. SSSA Special Publication 35. Madison, WI, USA: SSSA and ASA, pp. 107124.
Dick, RP, Rasmussen, PE and Krele, EA (1988) Influence of long-term residue management on soil enzyme activities in relation to soil chemical properties of a wheat-fallow system. Biology and Fertility of Soils 6, 159164.
Fageria, NK and Barbosa Filho, MP (2001) Nitrogen use efficiency in lowland rice genotypes. Communications in Soil Science and Plant Analysis 32, 20792089.
Fageria, NK, Baligar, VC and Jones, CA (2010) Growth and Mineral Nutrition of Field Crops, 3rd Edn. New York, USA: Marcel Dekker, Inc.
Fan, JB, Zhang, YL, Turner, D, Duan, YH, Wang, DS and Shen, QR (2010) Root physiological and morphological characteristics of two rice cultivars with different nitrogen-use efficiency. Pedosphere 20, 446455.
Finkenbein, P, Kretschmer, K, Kuka, K, Klotz, S and Heilmeier, H (2013) Soil enzyme activities as bioindicators for substrate quality in revegetation of a subtropical coal mining dump. Soil Biology and Biochemistry 56, 8789.
Frankeberger, WT and Johanson, JB (1983) Method of measuring invertase activity in soils. Plant and Soil 74, 301311.
García-Ruiz, R, Ochoa, V, Hinojosa, MB and Carreira, JA (2008) Suitability of enzyme activities for the monitoring of soil quality improvement in organic agricultural systems. Soil Biology and Biochemistry 40, 21372145.
Guan, SY (1986) Soil enzyme and research method. In Guan, SY (ed.), Measurement of Soil Enzyme. Beijing, China: China Agricultural Press, pp. 274279.
Gupta, VVSR and Germida, JJ (1988) Distribution of microbial biomass and its activity in different soil aggregate size classes as affected by cultivation. Soil Biology and Biochemistry 20, 777786.
Habtegebrial, K, Mersha, S and Habtu, S (2013) Nitrogen and sulphur fertilizers effects on yield, nitrogen uptake and nitrogen use efficiency of upland rice variety on irrigated Fulvisols of the Afar region, Ethiopia. Journal of Soil Science and Environmental Management 4, 6270.
Hartmann, A, Schmid, M, van Tuinen, D and Berg, G (2009) Plant-driven selection of microbes. Plant and Soil 321, 235257.
Högberg, P and Read, DJ (2006) Towards a more plant physiological perspective on soil ecology. Trends in Ecology and Evolution 21, 548554.
Iyyemperumal, K and Shi, W (2008) Soil enzyme activities in two forage systems following application of different rates of swine lagoon effluent or ammonium nitrate. Applied Soil Ecology 38, 128136.
Jackson, RB, Manwaring, JH and Caldwell, MM (1990) Rapid physiological adjustment of roots to localized soil enrichment. Nature 344, 5860.
Kandeler, E, Tscherko, D and Spiegel, H (1999) Long-term monitoring of microbial biomass, N mineralisation and enzyme activities of a Chernozem under different tillage management. Biology and Fertility of Soils 28, 343351.
Kato, Y, Kamoshita, A, Abe, J and Yamagishi, J (2007) Improvement of rice (Oryza sativa L.) growth in upland conditions with deep tillage and mulch. Soil and Tillage Research 92, 3044.
Klose, S and Tabatabai, MA (1999) Urease activity of microbial biomass in soils. Soil Biology and Biochemistry 31, 205211.
Kundu, DK, Ladha, JK and Lapitan-de Guzman, E (1996) Tillage depth influence on soil nitrogen distribution and availability in a rice lowland. Soil Science Society of America Journal 60, 11531159.
Liu, TQ, Fan, DJ, Zhang, XX, Chen, J, Li, CF and Cao, CG (2015) Deep placement of nitrogen fertilizers reduces ammonia volatilization and increases nitrogen utilization efficiency in no-tillage paddy fields in central China. Field Crops Research 184, 8090.
Lu, Y, Watanabe, A and Kimura, M (2002) Contribution of plant-derived carbon to soil microbial biomass dynamics in a paddy rice microcosm. Biology and Fertility of Soils 36, 136142.
Pandey, D, Agrawal, M and Bohra, JS (2014) Effects of conventional tillage and no tillage permutations on extracellular soil enzyme activities and microbial biomass under rice cultivation. Soil and Tillage Research 136, 5160.
Peng, S, Buresh, RJ, Huang, J, Zhong, X, Zou, Y, Yang, J, Wang, G, Liu, Y, Hu, R, Tang, Q, Cui, K, Zhang, F and Dobermann, A (2010) Improving nitrogen fertilization in rice by site-specific N management. A review. Agronomy for Sustainable Development 30, 649656.
Sakaigaichi, T, Morita, S, Abe, J and Yamaguchi, T (2007) Diurnal and phenological changes in the rate of nitrogen transportation monitored by bleeding in field-grown rice plants (Oryza sativa L.). Plant Production Science 10, 270276.
Schurr, U (1998) Xylem sap sampling–new approaches to an old topic. Trends in Plant Science 3, 293298.
Shen, AL, Zhang, XB, Liu, CZ and , AY (2000) Status of soil water permeability in the rice and wheat rotation area along Yellow River and improvement effect of deep digging and fertilization on soil permeability. Journal of Henan Agricultural Sciences 5, 2225.
Shu, S, Tang, X, Luo, X, Zhang, G, Li, G, Duan, M, Li, Y, Chen, L, Liao, Y, Yao, L and Ye, C (2013) Deep mechanized application of super rice special fertilizer increasing soil fertility and yield of super rice. Transactions of the Chinese Society of Agricultural Engineering 29, 914.
Sinsabaugh, RL, Lauber, CL, Weintraub, MN, Ahmed, B, Allison, SD, Crenshaw, C, Contosta, AR, Cusack, D, Frey, S, Gallo, ME, Gartner, TB, Hobbie, SE, Holland, K, Keeler, BL, Powers, JS, Stursova, M, Takacs-Vesbach, C, Waldrop, MP, Wallenstein, MD, Zak, DR and Zeglin, LH (2008) Stoichiometry of soil enzyme activity at global scale. Ecology Letters 11, 12521264.
Soejima, H, Sugiyama, T and Ishihara, K (1992) Changes in cytokinin activities and mass spectrometric analysis of cytokinins in root exudates of rice plant (Oryza sativa L.) comparison between cultivars Nipponbare and Akenohoshi. Plant Physiology 100, 17241729.
Sommer, SG, Schjoerring, JK and Denmead, OT (2004) Ammonia emission from mineral fertilizers and fertilized crops. Advances in Agronomy 82, 557622.
Spohn, M and Kuzyakov, Y (2014) Spatial and temporal dynamics of hotspots of enzyme activity in soil as affected by living and dead roots-a soil zymography analysis. Plant and Soil 379, 6777.
Wada, S and Kudo, T (1966) Varietal difference of yield of rice plants in response to the direct supply of fertilizers to the rhizosphere of the growing plants: I. Analysis of yield and its components. Japanese Journal of Crop Science 34, 425430.
Wang, WJ, Katayama, K and Takeda, T (1991) Study on applicability of nitrogen-deep placement culture from the view point of productivity of various rice cultivars in warmer area. Japanese Journal of Crop Science 60, 5764.
Wu, W, Zhang, S, Zhao, J, Wu, G, Li, Z and Xia, J (2007) Nitrogen uptake, utilization and rice yield in the north rimland of double-cropping rice region as affected by different nitrogen management strategies. Plant Nutrition and Fertilizer Science 13, 757764.
Xing, GX and Zhu, ZL (2000) An assessment of N loss from agricultural fields to the environment in China. Nutrient Cycling in Agroecosystems 57, 6773.
Yang, A, Mu, X, Li, M, Ye, M and Yu, H (2003) Effect of nitrogen application on the senescence of flag leaf and yield of rice cultivated in aerobic soil. Journal of Nanjing Agricultural University 27, 126129.
Yin, CY, Yang, HX, Du, YX, Zhang, J, Li, JZ, Sun, HZ, Peng, T and Zhao, QZ (2013) Difference of bleeding intensity in different parts of rice plant and its relationship with grain plumpness. Acta Agronomica Sinica 39, 153163.
Yoshida, R and Oritani, T (1974) Studies on nitrogen metabolism in crop plants: XIII. Effects of nitrogen top-dressing on cytokinin content in the root, exudate of rice plant. Japanese Journal of Crop Science 43, 4751.
Zadoks, JC, Chang, TT and Konzak, CF (1974) A decimal code for the growth stages of cereals. Weed Research 14, 415421.
Zantua, MI, Dumenil, LC and Bremner, JM (1977) Relationships between soil urease activity and other soil properties. Soil Science Society of America Journal 41, 350352.
Zhang, H and Forde, BG (1998) An Arabidopsis MADS box gene that controls nutrient-induced changes in root architecture. Science 279, 407409.
Zhang, J, Zhang, Z, Du, YX, Li, JZ, Sun, HZ and Zhao, QZ (2012) Effects of different treatments of deep tillage and panicle fertilization on root activity, grain-filling dynamics and yield of rice (Oryza sativa L.). Scientia Agricultura Sinica 45, 41154122.
Zhao, QZ, Gao, EM, Huang, PS and Ling, QH (1999) Relation between bleeding potential in Neck of spike and source-sink quality of rice. Scientia Agricultura Sinica 32, 104106.
Zhao, QZ, Gao, EM, Huang, PS and Ling, QH (2001) The comparison and nitrogen nutrition regulations of bleeding in neck-Panicle node and basal internode of rice. Acta Agronomica Sinica 27, 103109.


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Effects of N top-dressing modes of panicle fertilization on soil enzymes activity and yield of rice (Oryza sativa L.)

  • Juan Liu (a1) (a2), Jing Zhang (a1) (a2), Xiaodong Chen (a1) (a2), Yanxiu Du (a1) (a2), Junzhou Li (a1) (a2), Hongzheng Sun (a1) (a2), Ting Peng (a1) (a2), Zeyu Xin (a1) (a2) and Quanzhi Zhao (a1) (a2)...


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