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Effects of daidzein on growth performance, blood metabolites and meat quality of finishing Xianan beef cattle

  • H. Liang (a1), X. H. Zhao (a1), K. Pan (a1), L. J. Xu (a1), Z. H. Yi (a1), J. Bai (a1), X. L. Qi (a2), Z. D. Chen (a1), X. Z. Song (a1), K. H. Ouyang (a1), C. J. Liu (a1) and M. R. Qu (a1)...


An experiment was conducted to determine the effects of supplementing different amounts of daidzein in a diet on the growth performance, blood biochemical parameters and meat quality of finishing beef cattle. Thirty finishing Xianan steers were distributed in three groups equilibrated by weight and fed three different dietary treatments (concentrate ratio = 80%): (1) control; (2) 500 mg/kg daidzein and (3) 1000 mg/kg daidzein, respectively. Steers were slaughtered after an 80-day feeding trial. Results showed that daidzein supplementation had no effect on the final body weight, average daily gain and feed conversion rate of steers. Steers fed with 1000 mg/kg daidzein had greater dry matter intake than those fed with control diets. Compared with the control group, the 1000 mg/kg daidzein group had a higher fat thickness, lower shear force and lightness. The pH, drip loss, cooking loss, redness (a*), yellowness (b*), moisture, ash, crude protein and intramuscular fat of the Longissimus dorsi muscle were unaffected by daidzein supplementation. Compared with the control group, the 1000 mg/kg daidzein group significantly increased the serum concentrations of insulin, free fatty acid and Glutamic-pyruvic transaminase. The 500 mg/kg daidzein group significantly increased the serum concentration of tetraiodothyronine compared with the control group. Supplemental daidzein did not affect the blood antioxidant ability and blood immune parameters in serum. In conclusion, daidzein supplementation above 500 mg/day modifies feed intake and metabolic and hormonal profile, with positive and negative effects on meat quality.


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Author for correspondence: M. R. Qu, E-mail:


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AOAC (Association of Official Analytical Chemists) (2000) Official Methods of Analysis, 17th Edn. Arlington, VA, USA: Association of Official Analytical Chemists.
Bauman, DE, Harvatine, KJ and Lock, AL (2011) Nutrigenomics, rumen-derived bioactive fatty acids, and the regulation of milk fat synthesis. Annual Review of Nutrition 31, 299319.
Belew, JB, Brooks, JC, McKenna, DR and Savell, JW (2003) Warner-Bratzler shear evaluations of 40 bovine muscles. Meat Science 64, 507512.
Blanco, C, Giráldez, JF, Morán, L, Mateo, J, Villalobos-Delgado, LH, Andrés, S and Bodas, R (2017) Effects of sunflower soap stocks on light lamb meat quality. Journal of Animal Science 95, 34553466.
Brown, MS, Krehbiel, CR, Galyean, ML, Remmenga, MD, Peters, JP, Hibbard, B, Robinson, J and Moseley, WM (2000) Evaluation of models of acute and subacute acidosis on dry matter intake, ruminal fermentation, blood chemistry, and endocrine profiles of beef steers. Journal of Animal Science 78, 31553168.
Chen, JY, Niu, L, Huang, M and Zhou, GH (2012) Major bio-factors affecting beef color stability. Scientia Agricultura Sinica 45, 33633372.
Choi, MS, Jung, UJ, Kim, MJ, Kim, JY, Park, SY, Jang, JY and Lee, MK (2005) Effect of genistein and daidzein on glucose uptake in isolated rat adipocytes; comparison with respective glycones. Journal of Food Science and Nutrition 10, 5257.
Dang, Z and Löwik, CW (2004) The balance between concurrent activation of ERs and PPARs determines daidzein-induced osteogenesis and adipogenesis. Journal of Bone and Mineral Research 19, 853861.
Foti, P, Erba, D, Riso, P, Spadafranca, A, Criscuoli, F and Testolin, G (2005) Comparison between daidzein and genistein antioxidant activity in primary and cancer lymphocytes. Archives of Biochemistry and Biophysics 433, 421427.
Franke, AA, Custer, LJ, Cerna, CM and Narala, KK (1994) Quantitation of phytoestrogens in legumes by HPLC. Journal of Agricultural and Food Chemistry 42, 19051913.
Guo, H, Han, Z and Wang, GJ (2002) Effect of supplementation of daidzein in diet on the performance and related endocrine secretion in castrated piglets. Chinese Journal of Animal Science 38, 1718.
Kadegowda, AKG, Piperova, LS, Delmonte, P and Erdman, RA (2008) Abomasal infusion of butterfat increases milk fat in lactating dairy cows. Journal of Dairy Science 91, 23702379.
Kašparovská, J, Dadáková, K, Lochman, J, Hadrová, S, Křížová, L and Kašparovský, T (2017) Changes in equol and major soybean isoflavone contents during processing and storage of yogurts made from control or isoflavone-enriched bovine milk determined using LC–MS (TOF) analysis. Food Chemistry 222, 6773.
Khafipour, E, Krause, DO and Plaizier, JC (2009) A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation. Journal of Dairy Science 92, 10601070.
Kim, YH, Keeton, JT, Smith, SB, Berghman, LR and Savell, JW (2009) Role of lactate dehydrogenase in metmyoglobin reduction and color stability of different bovine muscles. Meat Science 83, 376382.
Koohmaraie, M and Geesink, GH (2006) Contribution of postmortem muscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system. Meat Science 74, 3443.
Kristensen, NB and Harmon, DL (2004) Effect of increasing ruminal butyrate absorption on splanchnic metabolism of volatile fatty acids absorbed from the washed reticulorumen of steers. Journal of Animal Science 82, 35493559.
Li, FN, Li, LL, Yang, HS, Yuan, XX, Zhang, B, Geng, MM, Xiao, CW and Yin, YL (2011) Regulation of soy isoflavones on weight gain and fat percentage: evaluation in a Chinese Guangxi minipig model. Animal: An International Journal of Animal Bioscience 5, 19031908.
Li, FF, Zhu, T, Zhu, Y, Gao, Y, Meng, L, Guo, FL and Zhang, Y (2015) Soybean isoflavone effects on growth performance, immune function, nutrient apparent digestibility and fecal microorganism in weaning piglets. Swine Production 5, 2529.
Liang, H, Xu, LJ, Zhao, XH, Bai, J, Chen, ZD, Zhou, S, Song, XZ, Ouyang, KH, Pan, K, Liu, CJ and Qu, MR (2018) Effect of daidzein on fermentation parameters and bacterial community of finishing Xianan cattle. Italian Journal of Animal Science 17, 950958.
Liggins, J, Mulligan, A, Runswick, S and Bingham, SA (2002) Daidzein and genistein content of cereals. European Journal of Clinical Nutrition 56, 961966.
Liu, Q, Lanari, MC and Schaefer, DM (1995) A review of dietary vitamin E supplementation for improvement of beef quality. Journal of Animal Science 73, 31313140.
Liu, X, Zhang, G and Zhang, W (2003) Effect of dietary addition cysteamine and daidzein on adipose metabolism in broilers. Journal of Northeast Agricultural University 34,171175.
Liu, X, Suzuki, N, Laxmi, YRS, Okamoto, Y and Shibutani, S (2012) Anti-breast cancer potential of daidzein in rodents. Life Sciences 91, 415419.
Liu, DY, He, SJ, Jin, EH, Liu, SQ, Tang, YG, Li, SH and Zhong, LT (2013) Effect of daidzein on production performance and serum antioxidative function in late lactation cows under heat stress. Livestock Science 152, 1620.
Lozupone, CA, Stombaugh, JI, Gordon, JI, Jansson, JK and Knight, R (2012) Diversity, stability and resilience of the human gut microbiota. Nature 489, 220230.
Lundh, T (1995) Metabolism of estrogenic isoflavones in domestic animals. Proceedings of the Society for Experimental Biology and Medicine 208, 3339.
Mishra, P, Kar, A and Kale, RK (2009) Prevention of chemically induced mammary tumorigenesis by daidzein in pre-pubertal rats: the role of peroxidative damage and antioxidative enzymes. Molecular and Cellular Biochemistry 325, 149157.
Mohammad-Shahi, M, Haidari, F, Rashidi, B, Saei, AA, Mahboob, S and Rashidi, MR (2011) Comparison of the effects of genistein and daidzein with dexamethasone and soy protein on rheumatoid arthritis in rats. BioImpacts: BI 1, 161170.
Morimoto, M, Watanabe, T, Yamori, M, Takebe, M and Wakatsuki, Y (2009) Isoflavones regulate innate immunity and inhibit experimental colitis. Journal of Gastroenterology and Hepatology 24, 11231129.
O'Keefe, M and Hood, DE (1982) Biochemical factors influencing metmyoglobin formation on beef from muscles of differing colour stability. Meat Science 7, 209228.
Ovadia, H, Haim, Y, Nov, O, Almog, O, Kovsan, J, Bashan, N, Benhar, M and Rudich, A (2011) Increased adipocyte s-nitrosylation targets anti-lipolytic action of insulin: relevance to adipose tissue dysfunction in obesity. Journal of Biological Chemistry 286, 3043330443.
Rehfeldt, C, Adamovic, I and Kuhn, G (2007) Effects of dietary daidzein supplementation of pregnant sows on carcass and meat quality and skeletal muscle cellularity of the progeny. Meat Science 75, 103111.
Ren, DP, Geng, ZC, Liu, SJ and Guo, J (2009) Effects of cysteamine and daidzein on gene expression of IGF-I mRNA in tissue of northeast fine-wool sheep. Chinese Journal of Animal Nutrition 21, 967973.
Ru, BR, Lou, CH, Qi, XL, Li, PF, Wang, ZB and Yang, QC (2006) Project progress report on new beef line breeding by introducing the Charolais blood into Nanyang cattle. China Cattle Science 75, 4043.
Šošic-Jurjević, B, Filipović, B, Ajdzanović, V, Brkić, D, Ristić, N, Stojanoski, MM, Nestorović, N, Trifunović, S and Sekulić, M (2007) A brief communication: subcutaneously administrated genistein and daidzein decrease serum cholesterol and increase triglyceride levels in male middle-aged rats. Experimental Biology and Medicine 232, 12221227.
Szkudelska, K, Szkudelski, T and Nogowski, L (2002) Daidzein, coumestrol and zearalenone affect lipogenesis and lipolysis in rat adipocytes. Phytomedicine 9, 338345.
Van Soest, PJ, Robertson, JB and Lewis, BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 35833597.
Vyn, TJ, Yin, X, Bruulsema, TW, Jackson, CC, Rajcan, I and Brouder, SM (2002) Potassium fertilization effects on isoflavone concentrations in soybean. Journal of Agricultural and Food Chemistry 50, 35013506.
Wang, G, Hang, T and Chen, J (1994) Effects of daidzein on muscle growth in broilers and mechanism involved. Guangdong Journal of Animal and Veterinary Science 3, 46.
Wang, Q, Garrity, GM, Tiedje, JM and Cole, JR (2007) Naïve Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology 73, 52615267.
Wang, XZ, Han, ZK and Wang, GJ (2008) Effects of ipriflavone on ruminal metabolism and concentration of sex hormones in rumen fluid of goat. Chinese Journal of Animal Nutrition 3, 16.
Wong, MC, Emery, PW, Preedy, VR and Wiseman, H (2008) Health benefits of isoflavones in functional foods? Proteomic and metabonomic advances. Inflammopharmacology 16, 235239.
Zhao, XH, Yang, ZQ, Bao, LB, Wang, CY, Zhou, S, Gong, JM, Fu, CB, Xu, LJ, Liu, CJ and Qu, MR (2015) Daidzein enhances intramuscular fat deposition and improves meat quality in finishing steers. Experimental Biology and Medicine 240, 11521157.
Zhao, XH, Zhou, S, Bao, LB, Song, XZ, Ouyang, KH, Xu, LJ, Pan, K, Liu, CJ and Qu, MR (2018) Response of rumen bacterial diversity and fermentation parameters in beef cattle to diets containing supplemental daidzein. Italian Journal of Animal Science 17, 643649.
Zhou, S, Zilberman, Y, Wassermann, K, Bain, SD, Sadovsky, Y and Gazit, D (2001) Estrogen modulates estrogen receptor alpha and beta expression, osteogenic activity, and apoptosis in mesenchymal stem cells (MSCs) of osteoporotic mice. Journal of Cellular Biochemistry 36(Suppl), 144155.


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Effects of daidzein on growth performance, blood metabolites and meat quality of finishing Xianan beef cattle

  • H. Liang (a1), X. H. Zhao (a1), K. Pan (a1), L. J. Xu (a1), Z. H. Yi (a1), J. Bai (a1), X. L. Qi (a2), Z. D. Chen (a1), X. Z. Song (a1), K. H. Ouyang (a1), C. J. Liu (a1) and M. R. Qu (a1)...


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