Hostname: page-component-8448b6f56d-m8qmq Total loading time: 0 Render date: 2024-04-25T06:16:05.948Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition

Published online by Cambridge University Press:  27 March 2009

T. Fujihara ,
E. R. Ørskov ,
P. J. Reeds  and
D. J. Kyle
T. Fujihara
Affiliation:
The Rowelt Research Institute, Bucksburn, Aberdeen, AB2 9SB
E. R. Ørskov
Affiliation:
The Rowelt Research Institute, Bucksburn, Aberdeen, AB2 9SB
P. J. Reeds
Affiliation:
The Rowelt Research Institute, Bucksburn, Aberdeen, AB2 9SB
D. J. Kyle
Affiliation:
The Rowelt Research Institute, Bucksburn, Aberdeen, AB2 9SB
Get access Rights & Permissions [Opens in a new window]

Summary

Two experiments were carried out to determine endogenous excretion of purine derivatives in steers and lambs, and to investigate the relationship between microbial nucleic acid input and urinary excretion of purine nitrogen.

The endogenous excretion of allantoin after conversion of hypoxanthine, xanthine and uric acid to allantoin, was calculated to be 72 and 26 mg/kg W0·75 per day in steers and lambs, respectively, when the dietary protein contained no nucleic acid nitrogen.

The excretion of purine derivatives increased linearly with increasing microbial nucleic acid input in lambs. The excretion of purine derivatives in excess of endogenous contribution was closely related to the theoretically expected values. The average recovery was calculated as 0·96 for one sheep and 1·0 for the other.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 109 , Issue 1 , August 1987 , pp. 7 - 12
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Antoniewicz, A. M., Heinemann, W. W. & Henks, E. M. (1980). The effect of changes in the intestinal flow of nucleic acids on allantoin excretion in the urine of sheep. Journal of Agricultural Science, Cambridge 95, 395–400.CrossRefGoogle Scholar
Antoniewicz, A. M. & Pisulewski, P. M. (1982). Measurement of endogenous allantoin excretion in sheep urine. Journal of Agricultural Science, Cambridge 98, 221–223.CrossRefGoogle Scholar
Blaxter, K. L. & Martin, A. K. (1962). The utilization of protein as a source of energy in fattening sheep. British Journal of Nutrition 16, 397–407.CrossRefGoogle ScholarPubMed
Davidson, J., Mathieson, J. & Boyne, A. W. (1970). The use of automation in determining nitrogen by the Kjeldahl method with final calculations by computer. Analyst, London 95, 181–193.CrossRefGoogle ScholarPubMed
Fujihara, T., Ørskov, E. R. & MacLeod, N. A. (1985). Effect of protein infusion on excretion of purine derivatives in steers nourished by intragastric nutrition. Proceedings of the Nutrition Society 44, 138A.Google Scholar
Giescke, D., Stangassinger, M. & Tiemeyer, W. (1984). Nucleic acid digestion and urinary purine metabolites in sheep nourished by intragastric infusions. Canadian Journal of Animal Science 64, (Supplement): VIth International Symposium on Ruminant Physiology, Banff, Alberta, 09 1984, pp. 144–145.Google Scholar
Greystone, J. E. (1968). In Human Growth (ed. Cheek, D. B.). Philadelphia, Lea & Febiger.Google Scholar
MacLeod, N. A., Corrigall, W., Stirton, R. A. & Ørskov, E. R. (1982). Intragastric infusion of nutrients in cattle. British Journal of Nutrition 47, 547–552.CrossRefGoogle ScholarPubMed
Maloiy, G. M. O., Kay, R. N. B., Goodall, E. D. & Topps, J. H. (1970). Digestion and nitrogen metabolism in sheep and red deer given large or small amounts of water and protein. British Journal of Nutrition 24, 843–855.CrossRefGoogle ScholarPubMed
Munro, H. N. & Fleck, A. (1969). Analysis of tissues and body fluids for nitrogenous constituents. In Mammalian Protein Metabolism (ed. Munro, H. N.) vol. 4, pp. 423–525. New York: Academic Press.CrossRefGoogle Scholar
Ørskov, E. R., Grubb, D. A., Wenham, G. & Corrigall, W. (1979). The sustenance of growing and fattening ruminants by intragastric infusion of volatile fatty acids and protein. British Journal of Nutrition 41, 535–558.CrossRefGoogle Scholar
Oser, B. L. (ed.) (1965). Hawk's Physiological Chemistry. 14th edn.New York: McGraw-Hill.Google Scholar
Razzaque, M. A. (1973). Synthesis and metabolism of nucleic and related compounds in sheep and red deer. Ph.D. thesis, University of Aberdeen.Google Scholar
Razzaque, M. A., Topps, J. H., Goodall, E. D. & Kay, R. N. B. (1973). Metabolism of nucleic acids by sheep and red deer. Proceedings of Nutrition Society 32, 59A.Google ScholarPubMed
Razzaque, M. A., Topps, J. H., Kay, R. N. B. & Brockway, J. M. (1981). Metabolism of the nucleic acids of rumen bacteria by preruminant and ruminant lambs. British Journal of Nutrition 45, 517–528.CrossRefGoogle ScholarPubMed
Roth, F. X. & Kikchgessner, M. (1980). Proceedings of 3rd EAAP Symposium on Protein Metabolism and Nutrition (ed. Oslage, H. J. and Rohr, K.), pp. 120–130.Google Scholar
Sibanda, S., Topps, J. H., Storm, E. & Ørskov, E. R. (1982). The excretion of allantoin by ruminants in relation to protein entering the abomasum. Proceedings of Nutrition Society 41, 75A.Google Scholar
Smith, R. H. (1975). In Digestion and Metabolism in the RumiTiant (ed. McDonald, I. W. and Warner, A. C.). Armidale: University of New England Press.Google Scholar
Smith, R. H. & McAllan, A. B. (1970). Nucleic acid metabolism in the ruminant. 2. Formation of microbial nucleic acids in the rumen in relation to the digestion of food nitrogen, and the fate of dietary nucleic acids. British Journal of Nutrition 24, 545–556.CrossRefGoogle Scholar
Smith, R. H. & McAllan, A. B. (1971). Nucleic acid metabolism in the ruminant. British Journal of Nutrition 25, 181–190.CrossRefGoogle ScholarPubMed
Storm, E., Brown, D. S. & Ørskov, E. R. (1983). The nutritive value of rumen micro-organisms in ruminants. British Journal of Nutrition 50, 479–485.CrossRefGoogle ScholarPubMed
Technicon Instruments Co. Ltd (1965). Technicon Methodology Sheet N-IIB.Basingstoke: Technicon Instruments Co. Ltd.Google Scholar
Topps, J. H. & Elliott, R. C. (1965). Relationship between concentrations of ruminal nucleic acids and excretion of purine derivatives by sheep. Nature, London 205, 498–499.CrossRefGoogle Scholar
Young, E. G. & Conway, C. F. (1942). On the estimation of allantoin by the Rimini-Schryner reaction. Journal of Biological Chemistry 142, 839–853.CrossRefGoogle Scholar