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Effect of balanced fertilizers on soil quality and lentil yield in Gangetic alluvial soils of India

  • S. R. Singh (a1), D. K. Kundu (a1), P. Dey (a2), Pushpa Singh (a3) and B. S. Mahapatra (a1)...


Declining pulse production has caused wide concern in recent years. A field experiment was conducted to investigate effects of balance fertilizers based on soil test values and targeted yield equations on soil biological activities, soil quality, nutrient acquisition and grain yield of lentil. Treatments included the use of farmyard manure (FYM), bio-inoculants and inorganic fertilizers at different rates and combinations. The results revealed significant improvement in nodulation, microbial counts, microbial biomass carbon (MBC), soil respiration, soil enzymes and soil organic carbon (SOC) with integrated approaches (i.e. fertilizer plus FYM or bio-inoculants); these improvements led to achievement of the specific target yield of 1.50 t/ha. Although the highest yield was achieved with fertilizers applied for a target yield of 2.0 t/ha, there was significant decline in nodulation, microbial counts, MBC, soil respiration, soil enzymes, SOC and soil quality. Correlation between soil quality index (SQI) and grain yield suggested a significant influence of balanced fertilization based on soil tests and target yield. Principal component analysis revealed the average contribution of soil quality indicators towards SQI was in descending order of SOC > acid phosphatase activity > total culturable fungi > available phosphorus > BMC, which are crucial for sustainable lentil production in alluvial soils.


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Author for correspondence: S. R. Singh, E-mail:


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Abbeddou, S, et al. (2011) Nutritional composition of lentil straw, vetch hay, olive leaves and saltbush leaves and their digestibility as measured in fat-tailed sheep. Small Ruminant Research 96, 126135.
Ai, C, et al. (2012) Responses of extracellular enzyme activities and microbial community in both the rhizosphere and bulk soil to long term fertilization practices in a fluvo-aquic soil. Geoderma 173–174, 330338.
Ali, M and Gupta, S (2012) Carrying capacity of Indian agriculture: pulse crops. Current Science 102, 874881.
Anderson, JPE (1982) Soil respiration. In Page, AL, Miller, RH and Keeney, DR (eds). Methods of Soil Analysis, Part 2. Madison, WI: ASA, SSSA, pp. 831871.
Andrews, M, et al. (1992) Nitrate effects on leaf growth of grain legumes prior to nodulation: species differences relate to nitrate uptake. In Ramsay, G and Middlefell-Williams, JE (eds). Proceedings of the 1st European Conference on Grain Legumes. Paris, France: L'Union Nationale Interprofessionnelle des Plantes Riches en Proteins, pp. 139140.
Andrews, SS, et al. (2002 a) On farm assessment of soil quality in California's central valley. Agronomy Journal 94, 1223.
Andrews, SS, Karlen, DL and Mitchell, JP (2002 b) A comparison of soil quality indexing methods for vegetable production systems in Northern California. Agriculture, Ecosystems and Environment 90, 2545.
AOAC (Association of Official Analytical Chemists) (1999) Official Methods of Analysis, 16th edn. Gaithersburg, Maryland: Association of Official Analytical Chemists.
Basu, TK and Bansyopadhyay, S (1990) Effect of Rhizobium inoculation and nitrogen application on some yields attributes of mung. Environment and Ecology 8, 650654.
Bera, R, et al. (2006) Targeted yield concept and a framework of fertilizers recommendation in irrigated rice domains of subtropical India. Journal of Zhejiang University Science B7, 963968.
Bremer, E, van Kessel, C and Karamanos, R (1989) Inoculant, phosphorus and nitrogen responses of lentil. Canadian Journal of Soil Science 69, 691701.
Chang, E-H, Chung, RS and Tsai, YW (2007) Effect of different application rates of organic fertilizer on soil enzyme activity and microbial population. Soil Science & Plant Nutrition 53, 132140.
Chen, XP, et al. (2010) Optimizing soil nitrogen supply in the root zone to improve maize management. Soil Science Society of America Journal 74, 13671373.
Chinnadurai, C, Gopalaswamy, G and Balachandar, D (2014) Long term effects of nutrient management regimes on abundance of bacterial genes and soil biochemical processes for fertility sustainability in a semi-arid tropical Alfisol. Geoderma 232–243, 563752.
Chivenge, P, Vanlauwe, B and Six, J (2011) Does the combined application of organic and mineral nutrient sources influence maize productivity? A meta-analysis. Plant and Soil 342, 130.
Choudhary, HR, et al. (2011) Effect of organic sources and chemical fertilizers on productivity of mungbean. Journal of Food Legumes 24, 324326.
Cochran, WG and Cox, GM (1957) Experimental Designs, 2nd edn. New York: John Wiley and Sons.
Contesto, C, et al. (2008) Effects of rhizobacterial ACC deaminase activity on Arabidopsis indicate that ethylene mediates local root responses to plant growth-promoting rhizobacteria. Plant Science 175, 178189.
Cui, ZL, et al. (2010) In-season nitrogen management strategy for winter wheat: maximizing yields, minimizing environment impact in an over-fertilization context. Field Crops Research 116, 140146.
Daterao, SH, et al. (1990) Effect of Rhizobium seed inoculation of green gram with and without molybdenum on grain yield and nitrogen status of soil. PKV Research Journal 14, 7577.
Dhull, S, et al. (2004) Microbial biomass carbon and microbial activities of soils receiving chemical fertilizers and organic amendments. Archive of Agronomy and Soil Science 50, 641647.
Doran, JW and Parkin, TB (1994) Defining and assessing soil quality. In Doran, JW, Coleman, DC, Bezdicek, DF and Stewart, BA (eds). Defining Soil Quality for A Sustainable Environment. Special Publication No. 35. Madison, WI: SSSA, pp. 321.
Egamberdiyeva, D (2005) Plant-growth-promoting rhizobacte-ria isolated from a Calcisol in a semi-arid region of Uzbekistan: biochemical characterization and effectiveness. Journal of Plant Nutrition and Soil Science 168, 9499.
Elfstrand, S, Hedlund, K and Martensson, A (2007) Soil enzyme activities, microbial community composition and function after 47 years of continuous green manuring. Applied Soil Ecology 35, 610621.
Ellouze, W, et al. (2014) Soil fungal resources in annual cropping systems and their potential for management. BioMed Research International 2014, 115, Article ID 531824. doi: 10.1155/2014/531824.
Erskine, W, Muehlbaure, FJ and Short, RW (1990) Stages of development in lentil. Experimental Agriculture 26, 297302.
FAOSTAT (2016) Agricultural Data: Agriculture and Food Trade. Rome, Italy: FAO. Available at (Accessed 20 February 2018).
Gan, Y, et al. (2009) Adaptability of chickpea in northern high latitude areas – maturity responses. Agricultural and Forest Meteorology 149, 711720.
Geisseler, D and Horwath, WR (2009) Relationship between carbon and nitrogen availability and extracellular enzyme activities in soil. Pedobiologia 53, 8798.
Gregorich, EG, Drury, CF and Baldock, JA (2001) Changes in soil carbon under long-term maize in monoculture and legume-based rotation. Canadian Journal of Soil Science 81, 2131.
Gull, M, et al. (2004) Phosphorus uptake and growth promotion of chickpea by co-inoculation of mineral phosphate solubilization bacteria and a mixed culture. Australian Journal of Experimental Agriculture 44, 623628.
He, P, et al. (2009) Performance of an optimized nutrient management system for double-cropped wheat-maize rotations in north-central China. Agronomy Journal 101, 14891496.
Hoque, MM and Haq, MF (1994) Rhizobium inoculation and fertilization of lentil in Bangladesh. LENS Newsletter 21, 2930.
Hussain, I, et al. (1999) Adaptation of soil quality indices and application to three tillage systems in southern Illinois. Soil and Tillage Research 50, 237249.
Iqbal, MA, et al. (2016) Integrated use of Rhizobium leguminosarum, plant growth promoting rhizobacteria and enriched compost for improving growth, nodulation and yield of lentil (Lens culinaris medik.). Chilean Journal of Agricultural Research 72, 104110.
Jackson, ML (1973) Soil Chemical Analysis. New Delhi, India: Prentice Hall of India Pvt., Ltd.
Kanazawa, S, Asakawa, S and Takai, Y (1988) Effect of fertilizer and manure application on microbial numbers, biomass and enzymes activities in volcanic ash soils. Soil Science and Plant Nutrition 34, 429439.
Körschens, M, et al. (2013) Effect of mineral and organic fertilization on crop yield, nitrogen uptake, carbon and nitrogen balances, as well as soil organic carbon content and dynamics: results from 20 European long-term field experiments of the twenty-first century. Archives of Agronomy and Soil Science 59, 10171040.
Kumar, S, et al. (2013) Balanced fertilization along with farmyard manures enhances abundance of microbial groups and their resistance and resilience against heat stress in a semi-arid inceptisol. Communications in Soil Science and Plant Analysis 44, 22992313.
Kundu, R, Mandal, J and Majumder, A (2013) Growth and production potential of green gram (Vigna radiata). influenced by Rhizobium inoculation with different nutrient sources. International Journal of Agriculture, Environment and Biotechnology 6, 344350.
Kutschera, L (1960) Wurzelatlasmitteleuropaischer Ackerunkrauter und Kulturpflanzen. Frankfurt, Germany: DLG-Verlags-GmbH.
Ladha, JK, et al. (2011) Role of nitrogen fertilization in sustaining organic matter in cultivated soils. Journal of Environmental Quality 40, 17561766.
Lavanya, GR and Toms, B (2009) Association and interrelationship among yield contributing characters in mungbean. Journal of Food Legumes 22, 6567.
Li, J, et al. (2008) Effects of long-term combined application of organic and mineral fertilizers on microbial biomass, soil enzyme activities and soil fertility. March 2008. Agricultural Sciences in China 7, 336343.
Martin, JP (1950) Use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi. Soil Science 69, 215232.
Marzluf, GA (1997) Genetic regulation of nitrogen metabolism in the fungi. Microbiology and Molecular Biology Reviews 61, 1732.
Matus, A, et al. (1997) The influence of tillage and crop rotation on nitrogen fixation in lentil and pea. Canadian Journal of Plant Science 77, 197200.
Nleya, T, Walley, F and Vandenberg, A (2001) Response of four common bean cultivars to granular inoculant in a short season dryland production system. Canadian Journal of Plant Science 81, 385390.
Olsen, SR, et al. (1954) Estimation of Available Phosphorus in Soils by Extraction with Sodium Bicarbonate. United States Department of Agriculture Circular no. 939. Washington DC: USDA.
Omar, SA and Ismail, MA (1999) Microbial populations, ammonification and nitrification in soil treated with urea and inorganic salts. Folia Microbiologica 44, 205212.
Parkinson, D, Gray, TRG and Williams, ST (1971) Methods for Studying the Ecology of Soil Microorganisms. International Biological Programme Handbook 19. Oxford, UK: Blackwell Scientist Publications.
Pikovskaya, AI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiology 17, 362370.
Qi, YB, et al. (2009) Evaluating soil quality indices in an agricultural region of Jiangsu Province, China. Geoderma 149, 325334.
Ramamoorthy, B and Velayutham, M (1971) Soil Test Crop Response Correlation Work in India. World Soil Resources Report No. 41: 96-105, Rome, Italy: FAO.
Ramamurthy, V, et al. (2009) Soil-based fertilizer recommendations for precision farming. Current Science 97, 641647.
Rezaei, SA, Gilkes, RJ and Andrews, SS (2006) A minimum data set for assessing soil quality in rangelands. Geoderma 136, 229234.
Robertson, GP and Groffman, PM (2015) Nitrogen transformations. In Paul, EA (ed.). Soil Microbiology, Ecology and Biochemistry, 4th edn. Amsterdam, the Netherlands: Elsevier, pp. 421446.
Sarkar, GK, et al. (2014) Depletion of soil potassium under exhaustive cropping in Inceptisols and Alfisols. Communications in Soil Science and Plant Analysis 45, 6172.
Schneider, K and Anderson, L (2010) Yield Gap and Productivity Potential in Ethiopian Agriculture: Staple Grains & Pulses. EPAR Brief No. 98. Seattle, WA: Evans School Policy Analysis and Research (EPAR), University of Washington.
Schnurer, J and Rosswall, T (1982) Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter. Applied and Environmental Microbiology 43, 12561261.
Schoenholtz, SH, Van Miegroet, H and Burger, JA (2000) A review of chemical and physical properties as indicators of forest soil quality: challenges and opportunities. Forest Ecology and Management 138, 335356.
Shahzad, SM, et al. (2008) Integrated use of plant growth promoting bacteria and p-enriched compost for improving growth, yield and nodulation of chickpea. Pakistan Journal of Botany 40, 1735–1441.
Singh, HP, et al. (1998) Prospects of Indian agriculture with special reference to nutrient management under rainfed systems. In Swarup, A, Damodar Reddy, D and Prasad, RN (eds). Long Term Soil Fertility Management Through Integrated Plant Nutrient Supply. Bhopal, India: Indian Institute of Soil Science, pp. 3454.
Singh, SR, et al. (2015) Impact of balanced fertilization on nutrient acquisition, fibre yield of jute and soil quality in New Gangetic alluvial soils of India. Applied Soil Ecology 92, 2434.
Singh, SR, et al. (2017) Identification of minimum data set under balanced fertilization for sustainable rice production and maintaining soil quality in alluvial soils of Eastern India. Communications in Soil Science and Plant Analysis 48, 21702192.
Soil Survey Staff (1998) Keys to Soil Taxonomy, 8th edn. Washington DC: USDA National Conservation Service.
Srinivasrao, C, et al. (2012) Long-term effects of soil fertility management on carbon sequestration in a rice–lentil cropping system of the Indo-Gangetic Plains. Soil Science Society of America Journal 76, 168178.
Stark, C, et al. (2007) Influence of organic and mineral amendments on microbial soil properties and processes. Applied Soil Ecology 35, 7993.
Subbiah, BV and Asija, GL (1956) A rapid procedure for estimation of available nitrogen in soils. Current Science 25, 259260.
Tabatabai, MA (1982) Soil enzymes. In Page, AL, Miller, RH and Keeney, DR (eds). Methods of Soil Analysis Part 2: Chemical and Microbiological Properties. New York: Academic Press, pp. 903947.
Tabatabai, MA and Bremner, JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry 1, 301307.
Vance, ED, Brookes, PC and Jenkinson, DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry 19, 703707.
Walkley, A and Black, CA (1934) An examination of Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science 37, 2938.
Wander, MM and Bollero, GA (1999) Soil quality assessment of tillage impacts in Illinois. Soil Science Society of America Journal 63, 961971.
Wold, S, Esbensen, K and Geladi, P (1987) Principal component analysis. Chemometrics and Intelligent Laboratory Systems 2, 3752.
Yang, JY, et al. (2007) Residual soil nitrogen in soil landscapes of Canada as affected by land use practices and agricultural policy scenarios. Land Use Policy 24, 8999.
Yang, L, et al. (2008) Fertilization regulates soil enzymatic activity and fertility dynamics in a cucumber field. Scientia Horticulturae 116, 2126.
Zafar, M, et al. (2012) Effect of plant growth-promoting rhizobacteria on growth, nodulation and nutrient accumulation of lentil under controlled conditions. Pedosphere 22, 848859.
Zhong, WH and Cai, ZC (2007) Long-term effects of inorganic fertilizers on microbial biomass and community functional diversity in a paddy soil derived from quaternary red clay. Applied Soil Ecology 36, 8491.



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