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Comparison of supplemental cobalt form on fibre digestion and cobalamin concentrations in cattle

  • R. C. WATERMAN (a1), W. L. KELLY (a1), C. K. LARSON (a2) and M. K. PETERSEN (a1)

Summary

Cobalt (Co) is essential for rumen microbial metabolism to synthesize methane, acetate and methionine. It also serves as a structural component of vitamin B12 (cobalamin), which functions as a coenzyme in energy metabolism. A study was conducted to determine if Co form (carbonate v. glucoheptonate) supplemented above the National Research Council requirements would improve digestibility of a low-quality forage diet and change serum cobalamin concentrations. Nineteen ruminally cannulated cows (577 ± 13 kg) were fed individually in a completely randomized experimental design. Cows were fed a grass hay diet that contained (79·2 g/kg crude protein, 565 g/kg total digestible nutrients, 633·2 g/kg neutral detergent fibre (NDF), 874·2 g/kg dry matter) at a rate of 0·02% of body weight on a as fed basis for a 62-day study, which consisted of three periods; acclimation (AC), treatment (TR) and residual (RE). Measurements taken in the AC period were used as covariates for analysis in the TR and RE periods. Cows were stratified by age (5 ± 0·4 years) and lactational history, and assigned to receive 12·5 mg supplemental Co in one of two forms: (1) 27·2 mg of Co carbonate (CC, n = 11 cows) or (2) 50 mg of Co glucoheptonate (CGH, n = 8 cows). Supplement was administered daily via a gelatin capsule placed directly into the rumen 2 h after feeding. During the last 96 h of each period, forage digestibility was measured using an in situ nylon bag technique. Blood samples were collected 4 and 6 h following feeding, and 24 h before the end of each period. A treatment × period interaction was detected for in situ organic matter (OM) disappearance at 96 h; (TR period: 684 and 708 ± 81 g/kg; RE period: 676 and 668 ± 75 g/kg, for CC and CGH, respectively). Once inclusion of Co in the CGH group was removed, OM disappearance was reduced by 4·01% compared with 0·82% in the CC cows. The NDF disappearance (OM basis) was less for the TR compared with the RE at 48 h (629 and 652 ± 39 g/kg, respectively). However, by 96 h NDF disappearance was greater for TR than the RE (704 and 689 ± 44 g/kg; respectively). No differences were detected for cobalamin serum concentrations or rate of fibre fermentation. The outcomes of the current research signify that there may be a slight residual effect of Co supplementation on fermentation; there was also an indication that Co source may enhance the overall extent of fermentation.

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Corresponding author

*To whom all correspondence should be addressed. Email: richard.waterman@ars.usda.gov

References

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Ammerman, C. B., Henry, P. R. & Loggins, P. R. (1982). Cobalt bioavbailablity in sheep. Journal of Animal Science 55, 403 (Abst.).
Bhatti, S. A. & Firkins, J. L. (1995). Kinetics of hydration and functional specific gravity of fibrous feed by-products. Journal of Animal Science 73, 14491458.
Brockman, R. P. (1993). Glucose and short-chain fatty acid metabolism. In Quantitative Aspects of Ruminant Digestion and Metabolism (Eds Forbes, J. M. & France, J.), pp. 249263. Wallingford, UK: CABI.
Du, Z., Hemken, R. W., Jackson, J. A. & Trammell, D. S. (1996). Utilization of copper in copper proteinate, copper lysine, and cupric sulfate using the rat as an experimental model. Journal of Animal Science 74, 16571663.
Hussein, H. S., Fahey, G. C. Jr, Wolf, B. W. & Berger, L. L. (1994). Effects of cobalt on in vitro fiber digestion of forages and by-products containing fiber. Journal of Dairy Science 77, 34323440.
Kegley, E. B. & Spears, J. W. (1994). Bioavailability of feed-grade copper sources (oxide, sulfate, or lysine) in growing cattle. Journal of Animal Science 72, 27282734.
Kennedy, D. G., Young, P. B., McCaughey, W. J., Kennedy, S. & Blanchflower, W. J. (1991). Rumen succinate production may ameliorate the effects of cobalt–vitamin B-12 deficiency on methylmalonyl CoA mutase in sheep. Journal of Nutrition 121, 12361242.
King, B. C., Donnelly, M. K., Bergstrom, G. C., Walker, L. P. & Gibson, D. M. (2009). An optimized microplate assay system for quantitative evaluation of plant cell wall-degrading enzyme activity of fungal culture extracts. Biotechnology & Bioengineering 102, 10331044.
Lopez-Guisa, J. M. & Satter, L. D. (1992). Effect of copper and cobalt addition on digestion and growth in heifers fed diets containing alfalfa silage or corn crop residues. Journal of Dairy Science 75, 247256.
McDowell, L. R. (2000). Vitamins in Animal and Human Nutriton. Ames Iowa: Iowa State Press.
Miller, G. L., Blum, R., Glennon, W. E. & Burton, A. L. (1960). Measurement of carboxymethylcellulase activity. Analytical Biochemistry 1, 127132.
Nockels, C. F., DeBonis, J. & Torrent, J. (1993). Stress induction affects copper and zinc balance in calves fed organic and inorganic copper and zinc sources. Journal of Animal Science 71, 25392545.
NRC (2000). Nutrient Requirements of Beef Cattle. 7th revised edn. Washington, DC: National Academies Press.
Schwarz, F. J., Kirchgessner, M. & Stangl, G. I. (2000). Cobalt requirement of beef cattle – feed intake and growth at different levels of cobalt supply. Journal of Animal Physiology & Animal Nutrition 83, 121131.
Seal, C. J., Parker, D. S. & Avery, P. J. (1992). The effect of forage and forage concentrate diets on rumen fermentation and metabolism of nutrients by the mesenteric- and portal-drained viscera in growing steers. British Journal of Nutrition 67, 355370.
Smith, R. M. (1987). Cobalt. In Trace Elements in Human and Animal Nutrition, 5th edn (Ed, Mertz, W.), pp. 143183. San Diego, CA: Academic Press.
Somers, G. F. (1973). The affinity of onion cell walls for calcium ions. American Journal of Botany 60, 987990.
Somers, M. & Gawthorne, J. M. (1969). The effect of dietary cobalt intake on the plasma vitamin B12 concentration of sheep. Australian Journal of Experimental Biology and Medical Science 47, 227233.
Stangl, G. I., Schwarz, F. J. & Kirchgessner, M. (1999). Moderate long-term cobalt-deficiency affects liver, brain and erythrocyte lipids and lipoproteins of cattle. Nutrition Research 19, 415427.
Stangl, G. I., Schwarz, F. J., Müller, H. & Kirchgessner, M. (2000). Evaluation of the cobalt requirement of beef cattle based on vitamin B12, folate, homocysteine and methylmalonic acid. British Journal of Nutrition 84, 645653.
Sutton, A. L. & Elliot, J. M. (1972). Effect of ratio of roughage to concentrate and level of feed intake on ovine ruminal vitamin B12 production. Journal of Nutrition 102, 13411346.
Tanner, R. S. & Wolfe, R. S. (1988). Nutritional requirements of methanomicrobium mobile. Applied & Environmental Microbiology 54, 625628.
Tiffany, M. E. & Spears, J. W. (2005). Differential responses to dietary cobalt in finishing steers fed corn-versus barley-based diets. Journal of Animal Science 83, 25802589.
Tiffany, M. E., Spears, J. W., Xi, L. & Horton, J. (2003). Influence of dietary cobalt source and concentration on performance, vitamin B12 status, and ruminal and plasma metabolites in growing and finishing steers. Journal of Animal Science 81, 31513159.
Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 35833597.
Wiley, J. S., Petersen, M. K., Clark, C. K., Ansotegui, R. P. & Lodman, D. W. (1991). The influence of timing and the addition of urea to supplements containing DL-methionine on ruminal fermentation and cow weight change in beef cows. Journal of Animal Science 69, 46174627.
Xiao, Z., Storms, R. & Tsang, A. (2004). Microplate-based filter paper assay to measure total cellulase activity. Biotechnology & Bioengineering 88, 832837.
Xiao, Z., Storms, R. & Tsang, A. (2005). Microplate-based carboxymethylcellulose assay for endoglucanase activity. Analytical Biochemistry 342, 176178.

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Comparison of supplemental cobalt form on fibre digestion and cobalamin concentrations in cattle

  • R. C. WATERMAN (a1), W. L. KELLY (a1), C. K. LARSON (a2) and M. K. PETERSEN (a1)

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