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Chronic heat stress and feed restriction affects carcass composition and the expression of genes involved in the control of fat deposition in broilers

  • J. DE ANTONIO (a1), M. F. FERNANDEZ-ALARCON (a1), R. LUNEDO (a1), G. H. SQUASSONI (a2), A. L. J. FERRAZ (a3), M. MACARI (a1), R. L. FURLAN (a1) and L. R. FURLAN (a3)...
  • Please note a correction has been issued for this article.

Summary

Heat stress (HS) is among the major limiting factors to growth of broilers. Heat stress also results in changes in the characteristics of the carcass, such as an increase in fat deposition. The molecular mechanisms responsible for fat deposition in broilers as a response to HS remain unknown. The current study aimed to describe the molecular mechanisms associated with the effects of high temperature and feed restriction due to chronic heat exposure at 32 °C, and to describe the resulting changes in the growth performance and carcass characteristics of the broilers at 21 and 42 days of age. In the current study, 441 male Cobb-500® broilers were subjected to three treatments that differed in rearing temperature and feeding regime: chronic HS fed ad libitum (HS/AL), thermoneutral environment fed ad libitum (TN/AL) and TN and pair-feeding on the feed intake (FI) of the heat-exposed group (TN/PF). HS increased fat content in the breast and wings and decreased fat content in the legs, but did not influence abdominal fat. These effects occurred regardless of reducing consumption induced by HS. Furthermore, HS, independently of reduced FI, increased liver sterol-regulatory element-binding protein-1 (SREBP-1) mRNA in both ages and growth hormone receptor (GHR) mRNA at 42days, whereas feed restriction reduced GHR mRNA only at 21days. In conclusion, increased fat content in the breast and wings was accompanied by a higher gene expression of GHR and SREBP-1, suggesting the involvement of both genes in the control of fat deposition in broilers exposed to HS.

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*To whom all correspondence should be addressed. Email: cedral@caunesp.unesp.br

References

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Abu-Dieyeh, Z. H. M. (2006). Effect of high temperature per se on growth performance of broilers. International Journal of Poultry Science 5, 1921.
Anon (2003). Cobb 500 Broiler Management Guide. Siloam Springs, AR: Cobb-Vantress Inc.
Arnould, C. & Leterrier, C (2007). Welfare of chickens reared for meat production. Production Animales 20, 4145.
Baziz, H. A., Geraert, P. A., Padilha, J. C. F. & Guillaumin, S. (1996). Chronic heat exposure enhances fat deposition and modifies muscle and fat partition in broiler carcasses. Poultry Science 75, 505513.
Buyse, J. & Decuypere, E. (1999). The role of the somatotropic axis in the metabolism of the chicken. Domestic Animal Endocrinology 17, 245255.
Buyse, J., Decuypere, E., Darras, V. M., Vleurick, L. M., Kuhn, E. R. & Veldhuis, J. D. (2000). Food deprivation and feeding of broiler chickens is associated with rapid and interdependent changes in the somatotrophic and thyrotrophic axes. British Poultry Science 41, 107116.
Campos, D. M. B., Fernandez-Alarcon, M. F., Souza, F. A., Nogueira, W. C. L., Hada, F. H., Carneiro, P. R. O. & Macari, M. (2013). Is high protein diet a good nutrition strategy for broiler chickens reared at heat stress condition? In Energy and Protein Metabolism and Nutrition in Sustainable Animal Production, Vol. 134. (Eds. Oltjen, J. W., Kebreab, E. & Lapierre, H.), pp. 197198. Energy and Protein Metabolism and Nutrition in Sustainable Animal Production series, EAAP. Wageningen, the Netherlands: Wageningen Academic Publishers.
Cheng, T. K., Hamre, M. L. & Coon, C. N. (1997). Responses of broilers to dietary protein levels and amino acid supplementation to low protein diets at various environmental temperatures. The Journal of Applied Poultry Research 6, 1833.
Coussens, P. M., Colvin, C. J., Rosa, G. J. M., Laspiur, J. P. & Elftman, M. D. (2003). Evidence for a novel gene blood expression program in peripheral mononuclear cells from Mycobacterium avium subsp. Paratuberculosis-infected cattle. Infection and Immunity 71, 64876498.
De Faria Filho, D. E., Campos, D. M. B., Alfonso-Torres, K. A., Vieira, B. S., Rosa, P. S., Vaz, A. M., Macari, M. & Furlan, R. L. (2007). Protein levels for heat-exposed broilers: performance, nutrients digestibility, and energy and protein metabolism. International Journal of Poultry Science 6, 187194.
Désert, C., Duclos, M. J., Blavy, P., Lecerf, F., Moreews, F., Klopp, C., Aubry, M., Herault, F., Le Roy, P., Berri, C., Douaire, M., Diot, C. & Lagarrigue, S. (2008). Transcriptome profiling of the feeding-to-fasting transition in chicken liver. BMC Genomics 9, 611. Available from: https://doi.org/10.1186/1471-2164-9-611
Desvergne, B., Michalik, L. & Wahli, W. (2006). Transcriptional regulation of metabolism. Physiological Reviews 86, 465514.
Estevez, I. (2007). Density allowances for broilers: where to set the limits? Poultry Science 86, 12651272.
Food and Agriculture Organization of United Nations – FAO (2012). FAOSTAT Database. Rome, Italy: FAO. Available from: http://faostat3.fao.org/home/E (verified 18 August 2017).
Geraert, P. A., Padilha, J. C. F. & Guillaumin, S. (1996). Metabolic and endocrine changes induced by chronic heat exposure in broiler chickens: growth performance, body composition and energy retention. British Journal of Nutrition 75, 195204.
Gonzales, E., Buyse, J., Loddi, M. M., Takita, T. S., Buys, N. & Decuypere, E. (1998). Performance, incidence of metabolic disturbances and endocrine variables of food-restricted male broiler chickens. British Poultry Science 39, 671678.
Hausman, G. J., Barb, C. R., Fairchild, B. D., Gamble, J. & Lee-Rutherford, L. (2012). Expression of genes for interleukins, neuropeptides, growth hormone receptor, and leptin receptor in adipose tissue from growing broiler chickens. Domestic Animal Endocrinology 43, 260263.
Hillgartner, F. B., Salati, L. M. & Goodridge, A. G. (1995). Physiological and molecular mechanisms involved in nutritional regulation of fatty acid synthesis. Physiological Reviews 75, 4776.
Kita, K. (1998). Refeeding increases hepatic insulin-like growth factor-I (IGF-I) gene expression and plasma IGF-I concentration in fasted chicks. British Poultry Science 39, 679682.
Lara, L. J. & Rostagno, M. H. (2013). Impact of heat stress on poultry production. Animals 3, 356369.
Livak, K. J. & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T)) method. Methods 25, 402408.
Madsen, S. A., Chang, L.-C., Hickey, M.-C., Rosa, G. J. M., Coussens, P. M. & Burton, J. L. (2004). Microarray analysis of gene expression in blood neutrophils of parturient cows. Physiological Genomics 16, 212221.
Mignon-Grasteau, S., Moreri, U., Narcy, A., Rousseau, X., Rodenburg, T. B., Tixier-Boichard, M. & Zerjal, T (2015). Robustness to chronic heat stress in laying hens: a meta-analysis. Poultry Science 94, 586600.
Oliveira Neto, A. R. D., Oliveira, R. F. M. D., Donzele, J. L., Rostagno, H. S., Ferreira, R. A., Maximiano, H. D. C. & Gasparino, E. (2000). Effect of environment temperature on performance and carcass characteristics in broilers pair-fed and two levels of metabolizable energy. Revista Brasileira de Zootecnia 29, 183190.
Proszkowiec-Weglarz, M., Richards, M. P., Humphrey, B. D., Rosebrough, R. W. & McMurtry, J. P. (2009). AMP-activated protein kinase and carbohydrate response element binding protein: a study of two potential regulatory factors in the hepatic lipogenic program of broiler chickens. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 154, 6879.
Rhoads, R. P., Baumgard, L. H., Suagee, J. K. & Sanders, S. R. (2013). Nutritional interventions to alleviate the negative consequences of heat stress. Advances in Nutrition: An International Review Journal 4, 267276.
Richards, M. P., Poch, S. M., Coon, C. N., Rosebrough, R. W., Ashwell, C. M. & McMurtry, J. P. (2003). Feed restriction significantly alters lipogenic gene expression in broiler breeder chickens. The Journal of Nutrition 133, 707715.
Sahin, K., Sahin, N., Onderci, M., Gursu, F. & Cikim, G. (2002). Optimal dietary concentration of chromium for alleviating the effect of heat stress on growth, carcass qualities, and some serum metabolites of broiler chickens. Biological Trace Element Research 89, 5364.
SAS Institute Inc (2011). SAS/STAT® 9·3 User's Guide. Cary, NC: SAS Inst. Inc.
Schreibman, M. (2012). The Endocrinology of Growth, Development, and Metabolism in Vertebrates. New York, USA: Academic Press.
Shimano, H., Yahagi, N., Amemiya-Kudo, M., Hasty, A. H., Osuga, J.-I., Tamura, Y., Shionoiri, F., Iizuka, Y., Ohashi, K., Harada, K., Gotoda, T., Ishibashi, S. & Yamada, N. (1999). Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes. Journal of Biological Chemistry 274, 3583235839.
Silva, D. & Queiroz, A. D. (2002). Análise de Alimentos: Métodos Químicos e Biológicos, 3rd edn, Viçosa, MG, Brazil: Universidade Federal de Viçosa.
St-Pierre, N. R., Cobanov, B. & Schnitkey, G. (2003). Economic losses from heat stress by US livestock industries. Journal of Dairy Science 86 (Suppl), E52E77.
Toplu, H. D. O., Oral, D., Nazligul, A., Karaarslan, S., Kaya, M. & Yagin, O. (2014). Effects of heat conditioning and dietary ascorbic acid supplementation on growth performance, carcass and meat quality characteristics in heat-stressed broilers. Ankara Üniversitesi Veteriner Fakültesi Dergisi 61, 295302.
Vasilatos-Younken, R. (1995). Proposed mechanisms for the regulation of growth hormone action in poultry: metabolic effects. The Journal of Nutrition 125 (Suppl), 1783S1789S.
Wang, P. H., Ko, Y. H., Chin, H. J., Hsu, C., Ding, S. T. & Chen, C. Y. (2009). The effect of feed restriction on expression of hepatic lipogenic genes in broiler chickens and the function of SREBP1. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 153, 327331.
Yang, X., Zhuang, J., Rao, K., Li, X. & Zhao, R. (2010). Effect of early feed restriction on hepatic lipid metabolism and expression of lipogenic genes in broiler chickens. Research in Veterinary Science 89, 438444.
Zhang, Z. Y., Jia, G. Q., Zuo, J. J., Zhang, Y., Lei, J., Ren, L. & Feng, D. Y. (2012). Effects of constant and cyclic heat stress on muscle metabolism and meat quality of broiler breast fillet and thigh meat. Poultry Science 91, 29312937.
Zhao, R., Muehlbauer, E., Decuypere, E. & Grossmann, R. (2004). Effect of genotype-nutrition on growth and somatotropic gene expression in the chicken. General and Comparative Endocrinology 136, 211.
Zuo, J., Xu, M., Abdullahi, Y. A., Ma, L., Zhang, Z. & Feng, D. (2015). Constant heat stress reduces skeletal muscle protein deposition in broilers. Journal of the Science of Food and Agriculture 95, 429436.

Chronic heat stress and feed restriction affects carcass composition and the expression of genes involved in the control of fat deposition in broilers

  • J. DE ANTONIO (a1), M. F. FERNANDEZ-ALARCON (a1), R. LUNEDO (a1), G. H. SQUASSONI (a2), A. L. J. FERRAZ (a3), M. MACARI (a1), R. L. FURLAN (a1) and L. R. FURLAN (a3)...
  • Please note a correction has been issued for this article.

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