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Assessment of polymorphisms in mysostatin gene and their allele substitution effects showed weak association with growth traits in Iranian Markhoz goats

Published online by Cambridge University Press:  08 December 2016

K. KHANI ,
A. ABDOLMOHAMMADI ,
S. FOROUTANIFAR  and
A. ZEBARJADI
K. KHANI
Affiliation:
Department of Animal Science, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
A. ABDOLMOHAMMADI*
Affiliation:
Department of Animal Science, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
S. FOROUTANIFAR
Affiliation:
Department of Animal Science, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
A. ZEBARJADI
Affiliation:
Department of Agronomy Science, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
*
*To whom all correspondence should be addressed. Email: alirezaam@razi.ac.ir
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Summary

Polymorphisms in the myostatin (MSTN) gene were detected in 150 female Iranian Markhoz goats. Two 573 base pairs (bp) and 475 bp fragments of the MSTN gene, which contains a deletion 5 bp indel (206 TTTTA/), in the region of exon 1 encoding the 5′ untranslated region (UTR) of the MSTN transcript, and two single-nucleotide polymorphisms (SNPs) of substitution (339T/A, 169T/G) in exon 1 and 3 regions, respectively, were amplified. The polymerase chain reaction (PCR) products were digested separately using restriction enzyme endonuclease DraI, HinIII and HindIII. The digestion results indicated AA and AB genotypes in the region of exon 1 encoding the 5′UTR of the MSTN transcript, AA, AT and TT genotypes in exon 1 and TT, TG and GG genotypes in exon 3. The SNPs loci were in Hardy–Weinberg disequilibrium but the deletion locus showed equilibrium in the Markhoz goat population. Evaluation of associations between the polymorphisms with the studied growth traits showed that the AA and GG genotypes of exons 1 and 3 have a significant positive effect on weight at 6 months of age (W6) and average daily gain (ADG) traits, but genotypes in the region of exon 1 encoding the 5′UTR of the MSTN transcript did not have any significant effect on the studied growth traits. The statistical analyses showed a positive and significant effect of the 339A allele (exon 1) for W6 and negative and significant effect of the 169G allele (exon 3) for ADG trait. Therefore, these results suggest that the MSTN gene could be a potential candidate gene that affects ADG and W6 traits in goats. More studies are needed to simultaneously consider variants of this region in a larger population to better understand MSTN gene effects on the economic traits in goat.

Type
Animal Research Papers
Information
The Journal of Agricultural Science , Volume 155 , Issue 3 , April 2017 , pp. 519 - 526
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Copyright
Copyright © Cambridge University Press 2016 

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References

REFERENCES

Ansary, M., Tahmoorespur, M., Nassiry, M., Taheri, A. & Valeh, M. V. (2011). Polymorphism in intron-I of myostatin gene and its association with estimated breeding values of growth traits in Baluchi sheep (Ovis aries). The Indian Journal of Animal Sciences 81, 75–78.Google Scholar
Boman, I. A., Klemetsdal, G., Nafstad, O., Blichfeldt, T. & Vage, D. I. (2010). Impact of two myostatin (MSTN) mutations on weight gain and lamb carcass classification in Norwegian white sheep (Ovis aries). Genetics Selection Evolution 42, 4. DOI: 10.1186/1297-9686-42-4.Google Scholar
Esmailizadeh, A. K., Bottema, C. D. K., Sellick, G. S., Verbyla, A. P., Morris, C. A., Cullen, N. G. & Pitchford, W. S. (2008). Effects of the myostatin F94L substitution on beef traits. Journal of Animal Science 86, 1038–1046.Google Scholar
Grisolia, A. B., D'Angelo, G. T., Porto Neto, L. R., Siqueira, F. & Garcia, J. F. (2009). Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle. Genetics and Molecular Research 8, 822–830.Google Scholar
Hu, X., Gao, Y., Feng, C., Liu, Q., Wang, X., Du, Z., Wang, Q. & Li, N. (2009). Advanced technologies for genomic analysis in farm animals and its application for QTL mapping. Genetica 136, 371–386.Google Scholar
Johnson, P. L., McEwan, J. C., Dodds, K. G., Purchas, R. W. & Blair, H. T. (2005). A directed search in the region of GDF8 for quantitative trait loci affecting carcass traits in Texel sheep. Journal of Animal Science 83, 1988–2000.Google Scholar
Johnson, P. L., Dodds, K. G., Bain, W. E., Greer, G. J., McLean, N. J., McLaren, R. J., Galloway, S. M., Van Stijn, T. C. & McEwan, J. C. (2009). Investigations into the GDF8 g + 6723G-A polymorphism in New Zealand Texel sheep. Journal of Animal Science 87, 1856–1864.Google Scholar
Khani, K., Abdolmohammadi, A., Foroutanifar, S. & Zebarjadi, A. (2014). Association of polymorphism in 5′UTR and exon1 regions of myostatin gene with twining trait in Markhoz goat breed. Genetics in the 3rd Millennium 12, 3536–3543.Google Scholar
Kijas, J. W., McCulloch, R., Edwards, J. E. H., Oddy, V. H., Lee, S. H. & Van der Werf, J. (2007). Evidence for multiple alleles effecting muscling and fatness at the ovine GDF8 locus. BMC Genetics 8, 80. DOI: 10.1186/1471-2156-8-80.CrossRefGoogle ScholarPubMed
Li, X., Liu, Z., Zhou, R., Zheng, G., Gong, Y. & Li, L. (2008). Deletion of TTTTA in 5′ UTR of goat MSTN gene and its distribution in different population groups and genetic effect on bodyweight at different ages. Frontiers of Agriculture in China 2, 103–109.Google Scholar
Li, X. L., Wu, Z. L., Gong, Y. F., Liu, Y. Q., Liu, Z. Z., Wang, X. J., Xin, T. R. & Ji, Q. (2006). Single-nucleotide polymorphism identification in the caprine myostatin gene. Journal of Animal Breeding and Genetics 123, 141–144.Google Scholar
Lin, J., Arnold, H. B., Della-Fera, M. A., Azain, M. J., Hartzell, D. L. & Baile, C. A. (2002). Myostatin knockout in mice increases myogenesis and decreases adipogenesis. Biochemical and Biophysical Research Communications 291, 701–706.Google Scholar
Ma, K., Mallidis, C., Artaza, J., Taylor, W., Gonzalez-Cadavid, N. & Bhasin, S. (2001). Characterization of 5′-regulatory region of human myostatin gene: regulation by dexamethasone in vitro . American Journal of Physiology, Endocrinology and Metabolism 281, E1128–E1136.Google Scholar
McMahon, C. D., Popovic, L., Jeanplong, F., Oldham, J. M., Kirk, S. P., Osepchook, C. C., Wong, K. W. Y., Sharma, M., Kambadur, R. & Bass, J. J. (2003). Sexual dimorphism is associated with decreased expression of processed myostatin in males. American Journal of Physiology – Endocrinology and Metabolism 284, E377–E381.Google Scholar
McPherron, A. C., Lawler, A. M. & Lee, S. J. (1999). Regulation of anterior/posterior patterning of the axial skeleton by growth/differentiation factor 11. Nature Genetics 22, 260–264.Google Scholar
Miar, Y., Salehi, A. R., Aleyasin, S. A. & Raoufzadeh, S. (2011). Study of polymorphism in myostatin gene in Chaal, Zel and Zandi Iranian sheep Breeds. Journal of Animal Production (Journal of Agriculture) 13, 75–78. 33–40.Google Scholar
Mohammad Abadi, M. R., Askari, N., Baghizadeh, A. & Esmailizadeh, A. K. (2009). A directed search around caprine candidate loci provided evidence for microsatellites linkage to growth and cashmere yield in Rayini goats. Small Ruminant Research 81, 146–151.CrossRefGoogle Scholar
Rashidi, A., Bishop, S. C. & Matika, O. (2011). Genetic parameter estimates for pre-weaning performance and reproduction traits in Markhoz goats. Small Ruminant Research 100, 100–106.CrossRefGoogle Scholar
SAS Institute (2004). SAS User's Guide Version 9.1: Statistics. Cary, NC: SAS Institute Inc.Google Scholar
Schenkel, F. S., Miller, S. P., Ye, X., Moore, S. S., Nkrumah, J. D., Li, C., Yu, J., Mandell, I. B., Wilton, J. W. & Williams, J. L. (2005). Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of beef cattle. Journal of Animal Science 83, 2009–2020.Google Scholar
Singh, S. P., Kumar, R., Kumari, P., Kumar, S. & Mitra, A. (2014). Characterization of 5′ upstream region and investigation of TTTTA deletion in 5′ UTR of myostatin (MSTN) gene in Indian goat breeds. Animal Biotechnology 25, 55–68.Google Scholar
Visser, C., Van Marle-Köster, E., Snyman, M. A., Bovenhuis, H. & Crooijmans, R. P. M. A. (2013). Quantitative trait loci associated with pre-weaning growth in South African Angora goats. Small Ruminant Research 112, 15–20.CrossRefGoogle Scholar
Walling, G. A., Visscher, P. M., Wilson, A. D., McTeir, B. L., Simm, G. & Bishop, S. C. (2004). Mapping of quantitative trait loci for growth and carcass traits in commercial sheep populations. Journal of Animal Science 82, 2234–2245.CrossRefGoogle ScholarPubMed
Yeh, F. C., Yang, R. C., Boyle, T. B. J., Ye, Z. H. & Mao, J. X. (1999). POPGENE, the User-Friendly Shareware for Population Genetic Analysis. Canada: Molecular Biology and Biotechnology Centre, University of Alberta. Edmonton, AB, Canada: University of Alberta.Google Scholar
Zhang, C., Liu, Y., Xu, D., Wen, Q., Li, X., Zhang, W. & Yang, L. (2012). Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance. Molecular Biology Reports 39, 3081–3087.Google Scholar
Zhang, Z. J., Ling, Y. H., Wang, L. J., Hang, Y. F., Guo, X. F., Zhang, Y. H., Ding, J. P. & Zhang, X. R. (2013). Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds. Genetics and Molecular Research 12, 965–971.Google Scholar