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The APETALA2/ethylene-responsive factor transcription factor OsDERF2 negatively modulates drought stress in rice by repressing abscisic acid responsive genes

Part of: AGS Millet Collection

Published online by Cambridge University Press:  13 February 2017

Y. GUO ,
R. HUANG ,
L. DUAN  and
J. WANG
Y. GUO
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
R. HUANG
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081, China
L. DUAN*
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
J. WANG*
Affiliation:
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081, China
*
*To whom all correspondence should be addressed. Emails: wangjuan@caas.cn and duanlsh@cau.edu.cn
*To whom all correspondence should be addressed. Emails: wangjuan@caas.cn and duanlsh@cau.edu.cn
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Summary

APETALA2/ethylene-responsive factor (AP2/ERF) family transcription factors play a vital role in plant growth and in response to hormones and abiotic stresses. In the current research, it is reported that OsDERF2, one of the drought-responsive ERF, is a member of the DREB sub-family. OsDERF2 is a nuclear-localized protein and has transcriptional activity in yeast. Expression of OsDERF2 was induced by drought and inhibited by abscisic acid (ABA). However, OsDERF2 RNA interference (RNAi) knock-down transgenic lines enhanced tolerance to drought stress at seedling stage and were much more sensitive to ABA treatment, which may result from the increased ABA level in vivo. The basic leucine zipper (bZIP) transcription factor family plays an important role in the ABA signalling pathway of abiotic stress. Quantitative real-time polymerase chain reaction analysis revealed that the bZIP family gene OsbZIP20 and ABA-response gene OsABA45 were up-regulated 25 times and 120 times, respectively, in OsDERF2 RNAi knock-down lines under drought stress, which were up-regulated five and seven times in wild type under drought stress. The current data reveal that OsDERF2 negatively modulates drought stress response in an ABA-mediated pathway through regulating gene expression of other ABA-response transcription factors.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 155 , Issue 6 , August 2017 , pp. 966 - 977
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Copyright
Copyright © Cambridge University Press 2017 

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References

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