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Activities of key enzymes involved in starch synthesis in grains of wheat under different irrigation patterns

Published online by Cambridge University Press:  22 April 2009

Z. DAI ,
Y. YIN  and
Z. WANG
Z. DAI
Affiliation:
Biology Department, Dezhou University, Dezhou253023, Shandong, P. R. China Agronomy College, Shandong Agricultural University/National Key Laboratory of Crop Biology/Key Laboratory of Wheat Cultivation Physiology and Genetic Improvement, Ministry of Agriculture, Tai'an271018, Shandong, P. R. China
Y. YIN
Affiliation:
Agronomy College, Shandong Agricultural University/National Key Laboratory of Crop Biology/Key Laboratory of Wheat Cultivation Physiology and Genetic Improvement, Ministry of Agriculture, Tai'an271018, Shandong, P. R. China
Z. WANG*
Affiliation:
Agronomy College, Shandong Agricultural University/National Key Laboratory of Crop Biology/Key Laboratory of Wheat Cultivation Physiology and Genetic Improvement, Ministry of Agriculture, Tai'an271018, Shandong, P. R. China
*
*To whom all correspondence should be addressed. Email: zlwang@sdau.edu.cn
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Summary

It is generally accepted that sucrose phosphate synthase (SPS), sucrose synthase (SuSy), ADP-glucose pyrophosphorylase (AGPase), soluble starch synthase (SSS), granule-bound starch synthase (GBSS) and starch branching enzyme (SBE) play a key role in starch synthesis in wheat grains. Starch synthesis in wheat grains is influenced by genotype and environment. However, what is not known is the degree of variation in enzyme activities during starch accumulation of wheat cultivars field-grown in different water regimes. The present study was undertaken to determine whether irrigation patterns could cause differences in starch accumulation and activities of key enzymes involved in starch synthesis. Starch accumulation and related enzyme activities were investigated in two winter wheat varieties, JM20 and BY535, differing in grain starch content, under two irrigation patterns. Results showed that soil water deficit led to an increase at early grain filling and decrease during late grain filling in starch accumulation rate (SAR) and activities of key enzymes involved in starch synthesis, especially AGPase, SSS and SBE. Water deficit enhanced grain starch accumulation in two wheat cultivars, suggesting that rainfed treatments increase physiological activities during early grain filling and promote starch accumulation. Furthermore, the change of SAR is consistent with SuSy, AGPase, SSS and GBSS. The results suggest that these enzymes play a key role in starch synthesis, and the decrease of photosynthate produced in the source organ is not the factor inhibiting starch accumulation.

Type
Crops and Soils
Information
The Journal of Agricultural Science , Volume 147 , Issue 4 , August 2009 , pp. 437 - 444
Copyright
Copyright © Cambridge University Press 2009

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