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Phosphorus affects high-molecular-weight glutenin subunits and glutenin macropolymer size distribution in wheat grains

Published online by Cambridge University Press:  23 July 2013

Y. NI
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China Agricultural Bureau of Rencheng District, Jining City of Shandong Province, Jining 272100, Shandong, People's Republic of China
D. YANG
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
Z. WANG
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
Y. YIN
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
T. CAI
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
Z. DAI
Affiliation:
Biology Department, Dezhou University, Dezhou 253023, Shandong, People's Republic of China
S. YAN
Affiliation:
College of Plant Science, Anhui Science and Technology University, Fengyang 233100, Anhui, People's Republic of China
W. LI
Affiliation:
College of Plant Science, Anhui Science and Technology University, Fengyang 233100, Anhui, People's Republic of China
Corresponding
E-mail address:

Summary

Two winter wheat (Triticum aestivum L.) cultivars, Jimai20 and Shannong12, differing in phosphorus (P) utilization efficiency, were selected to study the effect of P application rate on changes in glutenin macropolymer (GMP) size distribution and the content of high-molecular-weight glutenin subunits (HMW-GS) in wheat grain. Four P levels (0, 40, 100 and 160 kg/ha) were applied under N1 (112·5 kg/ha) and N2 (225 kg/ha) conditions in the field, in 2008/09 and 2009/10. The results showed that increased P levels favoured HMW-GS synthesis under N1 conditions, but had a less pronounced effect under N2. When compared with the control, the volume proportions of <10 μm GMP particles in grains of both cultivars were significantly decreased, and those of >100 μm were increased in response to P application. The volume proportions of 10–100 μm GMP particles in the cultivars Jimai20 and Shannong12 were respectively lower and higher in response to P application than with no P fertilizer. At maturity, for both cultivars, total HMW-GS content was negatively correlated with GMP particle volume of <10 μm, but positively correlated with that of >100 μm. These observations suggest that both P and N affect protein synthesis in wheat grains and there exists a relationship between HMW-GS content and the synthesis of large GMP particles (>100 μm). The N×P interaction was the most important factor to regulate the HMW-GS and GMP contents.

Type
Crops and Soils Research Papers
Copyright
Copyright © Cambridge University Press 2013 

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References

Chen, E., Wang, Z., Yin, Y., Guo, J., Chen, X., Li, Y., Wang, P., Wu, G., Ni, Y., Cai, T., Yang, W. & Cao, L. (2013). Shading after anthesis in wheat influences the amount and relative composition of grain proteins. Journal of Agricultural Science, Cambridge 151, 4455.CrossRefGoogle Scholar
Deng, Z., Tian, J., Zhao, L., Zhang, Y. & Sun, C. (2008). High temperature-induced changes in high molecular weight glutenin subunits of Chinese winter wheat and its influences on the texture of Chinese noodles. Journal of Agronomy and Crop Science 194, 262269.CrossRefGoogle Scholar
Don, C., Lichtendonk, W., Plijter, J. J. & Hamer, R. J. (2003 a). Glutenin macropolymer: a gel formed by glutenin particles. Journal of Cereal Science 37, 17.CrossRefGoogle Scholar
Don, C., Lichtendonk, W. J., Plijter, J. J. & Hamer, R. J. (2003 b). Understanding the link between GMP and dough: from glutenin particles in flour towards developed dough. Journal of Cereal Science 38, 157165.CrossRefGoogle Scholar
Don, C., Lookhart, G., Naeem, H., Macritchie, F. & Hamer, R. J. (2005). Heat stress and genotype affect the glutenin particles of the glutenin macropolymer-gel fraction. Journal of Cereal Science 42, 6980.CrossRefGoogle Scholar
Don, C., Mann, G., Bekes, F. & Hamer, R. J. (2006). HMW-GS affect the properties of glutenin particles in GMP and thus flour quality. Journal of Cereal Science 44, 127136.CrossRefGoogle Scholar
D'ovidio, R. & Masci, S. (2004). The low-molecular-weight glutenin subunits of wheat gluten. Journal of Cereal Science 39, 321339.CrossRefGoogle Scholar
Dupont, F. M. & Altenbach, S. B. (2003). Molecular and Biochemical impacts of environmental factors on wheat grain development and protein synthesis. Journal of Cereal Science 38, 133146.CrossRefGoogle Scholar
Dupont, F. M., Hurkman, W. J., Vensel, W. H., Tanaka, C., Kothari, K. M., Chung, O. K. & Altenbach, S. B. (2006). Protein accumulation and composition in wheat grains: effects of mineral nutrients and high temperature. European Journal of Agronomy 25, 96107.CrossRefGoogle Scholar
Dupont, F. M., Chan, R. & Lopez, R. (2007). Molar fractions of high-molecular-weight glutenin subunits are stable when wheat is grown under various mineral nutrition and temperature regimens. Journal of Cereal Science 45, 134139.CrossRefGoogle Scholar
Fu, B. X. & Kovacs, M. I. P. (1999). Research note: rapid single-step procedure for isolating total glutenin proteins of wheat flour. Journal of Cereal Science 29, 113116.CrossRefGoogle Scholar
Goesaert, H., Brijs, K., Veraverbeke, W. S., Courtin, C. M., Gebruers, K. & Delcour, J. A. (2005). Wheat flour constituents: how they impact bread quality, and how to impact their functionality. Trends in Food Science and Technology 16, 1230.CrossRefGoogle Scholar
Gornall, A. G., Bardawill, C. J. & David, M. M. (1949). Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177, 751766.Google ScholarPubMed
Gupta, R. B., Khan, K. & Macritchie, F. (1993). Biochemical basis of flour properties in bread wheats. I. Effects of variation in the quantity and size distribution of polymeric protein. Journal of Cereal Science 18, 2341.CrossRefGoogle Scholar
el Haddad, L., Aussenac, T., Fabre, J. L. & Sarrafi, A. (1995). Relationship between polymeric glutenin and the quality characteristics for seven common wheats (Triticum aestivum) grown in the field and greenhouse. Cereal Chemistry 72, 598601.Google Scholar
Jia, Y. Q., Fabre, J. L. & Aussenac, T. (1996). Effects of growing location on response of protein polymerization to increased nitrogen fertilization for the common wheat cultivar Soissons: relationship with some aspects of the breadmaking quality. Cereal Chemistry 73, 526532.Google Scholar
Jiang, D., Yue, H., Wollenweber, B., Tan, W., Mu, H., Mu, Y., Bo, Y., Dai, T., Jing, Q. & Cao, W. (2009). Effects of post-anthesis drought and waterlogging on accumulation of high-molecular-weight glutenin subunits and glutenin macropolymers content in wheat grain. Journal of Agronomy and Crop Science 195, 8997.CrossRefGoogle Scholar
Khan, K., Frohberg, R., Olson, T. & Huckle, L. (1989). Inheritance of gluten protein components of high-protein hard red spring wheat lines derived from Triticum turgidum var. dicoccoides. Cereal Chemistry 66, 397401.Google Scholar
Lazaro, L., Abbate, P. E., Cogliatti, D. H. & Andrade, F. H. (2010). Relationship between yield, growth and spike weight in wheat under phosphorus deficiency and shading. Journal of Agricultural Science, Cambridge 148, 8393.CrossRefGoogle Scholar
Liang, T. B., Yin, Y. P., Cai, R. G., Yan, S. H., Li, W. Y., Geng, Q. H., Wang, P., Wu, Y. H., Li, Y. & Wang, Z.-L. (2008). HMW-GS accumulation and GMP size distribution in grains of Shannong12 grown in different soil conditions. Acta Agronomica Sinica 34, 21602167.CrossRefGoogle Scholar
Lindsay, M. P. & Skerritt, J. H. (1999). The glutenin macropolymer of wheat flour doughs: structure-function perspectives. Trends in Food Science and Technology 10, 247253.CrossRefGoogle Scholar
Liu, L., He, Z. H., Yan, J., Zhang, Y., Xia, X. C. & Pena, R. J. (2005). Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on mixographic properties in Chinese bread wheats. Euphytica 142, 197204.CrossRefGoogle Scholar
Ni, Y., Wang, Z., Yin, Y., Li, W., Yan, S. & Cai, T. (2012). Starch granule size distribution in wheat grain in relation to phosphorus fertilization. Journal of Agricultural Science, Cambridge 150, 4552.CrossRefGoogle Scholar
Payne, P. I., Law, C. N. & Muddy, E. E. (1980). Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theoretical and Applied Genetics 58, 113120.CrossRefGoogle Scholar
Spiertz, J. H. J., Hamer, R. J., Xu, H., Primo-martin, , Don, C. & Van der Putten, P. E. L. (2006). Heat stress in wheat (Triticum aestivum L.): effects on grain growth and quality traits. European Journal of Agronomy 25, 8995.CrossRefGoogle Scholar
Steffolani, M. E., Perez, G. T., Ribotta, P. D., Puppo, M. C. & Leon, A. E. (2008). Effect of transglutaminase on properties of glutenin macropolymer and dough rheology. Cereal Chemistry 85, 3943.CrossRefGoogle Scholar
Triboi, E., Abad, A., Michelena, A., Lloveras, J., Ollier, J. L. & Daniel, C. (2000). Environmental effects on the quality of two wheat genotypes. 1. Quantitative and qualitative variation of storage proteins. European Journal of Agronomy 13, 4764.CrossRefGoogle Scholar
Tronsmo, K. M., Faergestad, , Longva, A., Schofield, J. D. & Magnus, E. M. (2002). A study of how size distribution of gluten proteins, surface properties of gluten and dough mixing properties relate to baking properties of wheat flours. Journal of Cereal Science 35, 201214.CrossRefGoogle Scholar
Veraverbeke, W. S., Larroque, O. R., Bekes, F. & Delcour, J. A. (2000 a). In vitro polymerization of wheat glutenin subunits with inorganic oxidizing agents. I. Comparison of single-step and stepwise oxidations of high molecular weight glutenin subunits. Cereal Chemistry 77, 582588.CrossRefGoogle Scholar
Veraverbeke, W. S., Larroque, O. R., Bekes, F. & Delcour, J. A. (2000 b). In vitro polymerization of wheat glutenin subunits with inorganic oxidizing agents. II. Stepwise oxidation of low molecular weight glutenin subunits and a mixture of high and low molecular weight glutenin subunits. Cereal Chemistry 77, 589594.CrossRefGoogle Scholar
Wang, F. C., Zhu, J. B., Khan, K., O'Brienand, L. & Chen, W. Y. (2004). The composition and quantity of wheat glutenin subunits in relation to high molecular weight glutenin polymers and their effects on breadmaking quality. Journal of Chinese Cereals and Oils Association 19, 1317.Google Scholar
Weegels, P. L., van de Pijpekamp, A. M., Graveland, A., Hamer, R. J. & Schofield, J. D. (1996). Depolymerisation and re-polymerisation of wheat glutenin during dough processing. I. Relationships between glutenin macropolymer content and quality parameters. Journal of Cereal Science 23, 103111.CrossRefGoogle Scholar
Weegels, P. L., Hamer, R. J. & Schofield, J. D. (1997). Depolymerisation and re-polymerisation of wheat glutenin during dough processing. II. Changes in composition. Journal of Cereal Science 25, 155163.CrossRefGoogle Scholar
Wieser, H. & Kieffer, R. (2001). Correlations of the amount of gluten protein types to the technological properties of wheat flours determined on a micro-scale. Journal of Cereal Science 34, 1927.CrossRefGoogle Scholar
Yue, H. W., Qin, X. D., Dai, T. B., Jing, Q., Cao, W. X. & Jing, D. (2006). Effects of nitrogen rate on accumulation of HMW-GS and GMP in wheat grain. Acta Agronomica Sinica 32, 16781683.Google Scholar
Yue, H. W., Jiang, D., Dai, T. B., Qin, X. D., Jing, Q. & Cao, W. X. (2007). Effect of nitrogen application rate on content of glutenin macropolymer and high molecular weight glutenin subunits in grains of two winter wheat cultivars. Journal of Cereal Science 45, 248256.CrossRefGoogle Scholar
Yu, S. L. (1990). Wheat in Shandong Province. China Agriculture Press: Beijing, China.Google Scholar
Zadoks, J. C., Chang, T. T. & Konzak, C. F. (1974). A decimal code for the growth stages of cereals. Weed Research 14, 415421.CrossRefGoogle Scholar
Zhang, P. P., He, Z. H., Zhang, Y., Xia, X. C., Liu, J. J., Yan, J. & Zhang, Y. (2007). Pan bread and Chinese white salted noodle qualities of Chinese winter wheat cultivars and their relationship with gluten protein fractions. Cereal Chemistry 84, 370378.CrossRefGoogle Scholar
Zhang, P. P., He, Z. H., Zhang, Y., Xia, X. C., Chen, D. S. & Zhang, Y. (2008). Association between % SDS-unextractable polymeric protein (%UPP) and end-use quality in Chinese bread wheat cultivars. Cereal Chemistry 85, 696700.CrossRefGoogle Scholar
Zhao, G. C., He, Z. H. & Liu, L. H. (2004). Study on the coenhancing regulating effect of fertilization and watering on the main quality and yield in Zhongyou 9507 high gluten wheat (in Chinese with English abstract). Scientia Agricultura Sinica 37, 351356.Google Scholar
Zhao, H. X., Hu, S. W., Ji, W. Q., Xue, X. Z., Guo, A. G. & Mares, D. (2001). Study on relationship between the size distribution of glutenin polymeric protein and wheat flour mixing properties. Scientia Agricultura Sinica 34, 465468.Google Scholar
Zhao, Q., Li, Y., Li, W. Y., Wang, P., Chen, X. G., Yin, Y. P. & Wang, Z. L. (2011). Effects of water-nitrogen interaction on content of high molecular weight glutenin subunits and GMP size distribution in wheat cultivars of different genotypes. Scientia Agricultura Sinica 44, 15711584.Google Scholar
Zheng, P. Y. (1992). Introduction of Crop Physiology. Beijing: Beijing Agricultural University.Google Scholar
Zhu, J. & Khan, K. (2001). Effects of genotype and environment on glutenin polymers and bread making quality. Cereal Chemistry 78, 125130.CrossRefGoogle Scholar
Zhu, J. & Khan, K. (2002). Quantitative variation of HMW glutenin subunits from hard red spring wheats grown in different environments. Cereal Chemistry 79, 783786.CrossRefGoogle Scholar
Zhu, X. K., Li, C. Y., Jiang, Z. Q., Huang, L. L., Feng, C. N., Guo, W. S. & Peng, Y. X. (2012). Responses of phosphorus use efficiency, grain yield, and quality to phosphorus application amount of weak-gluten wheat. Journal of Integrative Agriculture 11, 11031110.CrossRefGoogle Scholar

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