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Mislabeling of an Invasive Vine (Celastrus orbiculatus) as a Native Congener (C. scandens) in Horticulture

  • David N. Zaya (a1), Stacey A. Leicht-Young (a2), Noel B. Pavlovic (a2), Christopher S. Hetrea (a1) and Mary V. Ashley (a1)...


The horticultural industry is an important source of invasive ornamental plant species, which is part of the motivation for an increased emphasis on using native alternatives. We were interested in the possibility that plants marketed in the midwestern United States as the native Celastrus scandens, or American bittersweet, were actually the difficult-to-distinguish invasive Celastrus orbiculatus (oriental bittersweet) or hybrids of the two species. We used nuclear microsatellite DNA loci to compare the genetic identities of 34 plants from 11 vendors with reference plants from wild populations of known species identity. We found that 18 samples (53%) were mislabeled, and 7 of the 11 vendors sold mislabeled plants. Mislabeled plants were more likely to be purchased through Internet or phone order shipments and were significantly less expensive than accurately labeled plants. Vendors marketed mislabeled plants under five different cultivar names, as well as unnamed strains. Additionally, the most common native cultivar, ‘Autumn Revolution,’ displays reproductive characteristics that diverge from the typical C. scandens, which could be of some concern. The lower price and abundance of mislabeled invasive plants introduces incentives for consumers to unknowingly contribute to the spread of C. orbiculatus. Revealing the potential sources of C. orbiculatus is critical for controlling further spread of the invasive vine and limiting its impact on C. scandens populations.


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Current address of first author: Illinois Natural History Survey, 1816 South Oak Street, Champaign, IL 61820


current address of second author: Rummel, Klepper and Kahl, 700 East Pratt Street, Suite 500, Baltimore, MD 21202

Associate Editor for this paper: Marie Jasieniuk, University of California, Davis.



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Ashton, IW, Lerdau, MT (2008) Tolerance to herbivory, and not resistance, may explain differential success of invasive, naturalized, and native North American temperate vines. Divers Distrib 14:169178
Bailey, R, inventor; Bailey Nurseries, Inc., assignee (2009) Mar 10. Celastrus plant named ‘Bailumn.’ US patent USPP19,811
Burghardt, KT, Tallamy, DW, Shriver, WG (2009) Impact of native plants on bird and butterfly biodiversity in suburban landscapes. Conserv Biol 23:219224
Del Serrone, P, Attorri, L, Gallinella, B, Gallo, FR, Federici, E, Palazzino, G (2006) Molecular identification of Panax ginseng C.A. Meyer in ginseng commercial products. Nat Prod Commun 1:11371140
Del Tredici, P (2014) Untangling the twisted tale of oriental bittersweet. Arnoldia 71:218
Drew, J, Anderson, N, Andow, D (2010) Conundrums of a complex vector for invasive species control: a detailed examination of the horticultural industry. Biol Invasions 12:28372851
Dreyer, GD, Baird, LM, Fickler, C (1987) Celastrus scandens and Celastrus orbiculatus—comparisons of reproductive potential between a native and introduced woody vine. B Torrey Bot Club 114:260264
EDDMapS. (2017). Early Detection & Distribution Mapping System. University of Georgia–Center for Invasive Species and Ecosystem Health. Accessed June 1, 2017
Falush, D, Stephens, M, Pritchard, JK (2003) Inference of population structure: extensions to linked loci and correlated allele frequencies. Genetics 164:15671587
Fan, LL, Zhu, S, Chen, HB, Yang, DH, Cai, SQ, Komatsu, K (2009) Identification of the botanical source of Stemonae Radix based on polymerase chain reaction with specific primers and polymerase chain reaction-restriction fragment length polymorphism. Biol Pharm Bull 32:16241627
Feng, T, Liu, S, He, XJ (2010) Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA. Electron J Biotechn 13. doi: 10.2225/vol13-issue1-fulltext-13
Fike, J, Niering, WA (1999) Four decades of old field vegetation development and the role of Celastrus orbiculatus in the northeastern United States. J Veg Sci 10:483492
Honjo, M, Ueno, S, Tsumura, Y, Handa, T, Washitani, I, Ohsawa, R (2008) Tracing the origins of stocks of the endangered species Primula sieboldii using nuclear microsatellites and chloroplast DNA. Conserv Genet 9:11391147
Hou, D (1955) A revision of the genus Celastrus . Ann Mo Bot Gard 42:215302
Johnson, LMK, Galloway, LF (2008) From horticultural plantings into wild populations: movement of pollen and genes in Lobelia cardinalis . Plant Ecol 197:5567
Lehan, NE, Murphy, JR, Thorburn, LP, Bradley, BA (2013) Accidental introductions are an important source of invasive plants in the continental United States. Am J Bot 100:12871293
Leicht, SA (2005). The Comparative Ecology of an Invasive Bittersweet Species (Celastrus orbiculatus) and Its Native Congener (C. scandens). Ph.D Dissertation. Storrs, CT: University of Connecticut. 162 p
Leicht-Young, SA, Latimer, AM, Silander, JA (2011) Lianas escape self-thinning: experimental evidence of positive density dependence in temperate lianas Celastrus orbiculatus and C. scandens . Perspect Plant Ecol 13:163172
Leicht-Young, SA, Pavlovic, NB (2015) Lianas as invasive species in North America. Pages 429442 in Schnitzer SA, Bongers F, Burnham RJ, Putz FE, eds. Ecology of Lianas. New York, NY: Wiley
Leicht-Young, SA, Pavlovic, NB, Grundel, R, Frohnapple, KJ (2007a) Distinguishing native (Celastrus scandens L.) and invasive (C. orbiculatus Thunb.) bittersweet species using morphological characteristics. J Torrey Bot Soc 134:441450
Leicht-Young, SA, Silander, JA, Latimer, AM (2007b) Comparative performance of invasive and native Celastrus species across environmental gradients. Oecologia 154:273282
LeRoy, A, Potter, E, Woo, HH, Heber, D, Hirsch, AM (2002) Characterization and identification of alfalfa and red clover dietary supplements using a PCR-based method. J Agric Food Chem 50:50635069
Lin, WY, Chen, LR, Lin, TY (2008) Rapid authentication of Bupleurum species using an array of immobilized sequence-specific oligonucleotide probes. Planta Med 74:464469
Mack, RN, Erneberg, M (2002) The United States naturalized flora: largely the product of deliberate introductions. Ann Mo Bot Gard 89:176189
Manel, S, Berthier, P, Luikart, G (2002) Detecting wildlife poaching: identifying the origin of individuals with Bayesian assignment tests and multilocus genotypes. Conserv Biol 16:650659
Manissorn, J, Sukrong, S, Ruangrungsi, N, Mizukami, H (2010) Molecular phylogenetic analysis of Phyllanthus species in Thailand and the application of polymerase chain reaction-restriction fragment length polymorphism for Phyllanthus amarus identification. Biol Pharm Bull 33:17231727
Mihalov, JJ, Marderosian, AD, Pierce, JC (2000) DNA identification of commercial ginseng samples. J Agric Food Chem 48:37443752
Patterson, DT (1974). The Ecology of Oriental Bittersweet, Celastrus orbiculatus, a Weedy Introduced Ornamental Vine. Ph.D Dissertation. Durham, NC: Duke University. 252 p
Pavlovic, NB, Leicht-Young, SA (2011) Are temperate mature forests buffered from invasive lianas? J Torrey Bot Soc 138:8592
Peters, WL, Meyer, MH, Anderson, NO (2006) Minnesota horticultural industry survey on invasive plants. Euphytica 148:7586
R Core Team (2016) R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing, Accessed December 1, 2016
Rachmayanti, Y, Leinemann, L, Gailing, O, Finkeldey, R (2009) DNA from processed and unprocessed wood: factors influencing the isolation success. Forensic Sci Int Genet 3:185192
Reichard, SH, Hamilton, CW (1997) Predicting invasions of woody plants introduced into North America. Conserv Biol 11:193203
Schuelke, M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233234
Srirama, R, Senthilkumar, U, Sreejayan, N, Ravikanth, G, Gurumurthy, BR, Shivanna, MB, Sanjappa, M, Ganeshaiah, KN, Shaanker, RU (2010) Assessing species admixtures in raw drug trade of Phyllanthus, a hepato-protective plant using molecular tools. J Ethnopharmacol 130:208215
Tallamy, DW (2007) Bringing Nature Home: How Native Plants Sutain Wildlife in Our Gardens. Portland, OR: Timber Press. 288 p
[USDA-NRCS] U.S. Department of Agriculture, Natural Resources Conservation Service (2017) The PLANTS Database. National Plant Data Team. Accessed June 1, 2017
Vongsak, B, Kengtong, S, Vajrodaya, S, Sukrong, S (2008) Sequencing analysis of the medicinal plant Stemona tuberosa and five related species existing in Thailand based on trnH-psbA chloroplast DNA. Planta Med 74:17641766
Wang, CZ, Li, P, Ding, JY, Peng, X, Yuan, CS (2007) Simultaneous identification of Bulbus Fritillariae Cirrhosae using PCR-RFLP analysis. Phytomedicine 14:628632
Whelan, RJ, Roberts, DG, England, PR, Ayre, DJ (2006) The potential for genetic contamination vs. augmentation by native plants in urban gardens. Biol Conserv 128:493500
Williams, PA, Timmins, SM (2003) Climbing spindle berry (Celastrus orbiculatus Thunb.) biology, ecology, and impacts in New Zealand. Wellington, New Zealand: New Zealand Government, Department of Conservation. 28 p
Xue, CY, Li, DZ, Lu, JM, Yang, JB, Liu, JQ (2006) Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii . Planta Med 72:12231226
Young, JA, Young, CG (1992) Seeds of Woody Plants in North America. Portland, OR: Dioscorides Press. 236 p
Zaya, DN (2013). Genetic Characterization of Invasion and Hybridization: A Bittersweet (Celastrus spp.) Story. Ph.D Dissertation. Chicago, IL: University of Illinois at Chicago. 90 p
Zaya, DN, Ashley, MV (2012) Plant genetics for forensic applications. Pages 3552 in Sucher NJ, Hennell JR, Carles MC, eds. Plant DNA Fingerprinting and Barcoding. New York, NY: Humana
Zaya, DN, Leicht-Young, SA, Pavlovic, NB, Feldheim, KA, Ashley, MV (2015) Genetic characterization of hybridization between native and invasive bittersweet vines (Celastrus spp.). Biol Invasions 17:29752988
Zhu, YY, Chen, HR, Fan, JH, Wang, YY, Li, Y, Chen, JB, Fan, JX, Yang, SS, Hu, LP, Leung, H, Mew, TW, Teng, PS, Wang, ZH, Mundt, CC (2000) Genetic diversity and disease control in rice. Nature 406:718722


Mislabeling of an Invasive Vine (Celastrus orbiculatus) as a Native Congener (C. scandens) in Horticulture

  • David N. Zaya (a1), Stacey A. Leicht-Young (a2), Noel B. Pavlovic (a2), Christopher S. Hetrea (a1) and Mary V. Ashley (a1)...


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