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Variation in the cuticular hydrocarbons of larvae of Anopheles gambiae and A. arabiensis

  • Greg I. Anyanwu (a1), Angela Phillips (a1) and David H. Molyneux (a1)

Abstract

Gas Liquid Chromatography was used to obtain profiles of cuticular hydrocarbons from single larvae of two species belonging to the Anopheles gambiae complex: A. gambiae and A. arabiensis. By applying discriminant analysis of hydrocarbons it was possible to separate the two populations with a 95% success rate.

The same analysis was used to achieve an average of 94% segregation among their strains. This analysis does not require preservation of specimen by liquid nitrogen or refrigeration and further identification by other procedures can be carried out on the specimens after cuticular hydrocarbon analysis. More importantly the technique permits analysis of single rather than pooled specimens used by earlier investigators.

Les profils de hydrocarbones de la cuticle des larves individuelles de deux espéces appartenant au complex Anopheles gambiae (A. gambiae et A. arabiensis) furent obtenus grâce á la chromatographie á phase gaseuse. En utilisant l'analyse du discriminant aux hydrocarbones il a étè possible de separer les deux populations avec un taux de succes de 95%. La même analyse a étè utilisé pour atteindre une moyenne de 94% de separation entre ces espéces. L'analyse ne necessite pas la preservation des specimen dans du nitrogene ou dans le frigidaire et d'autre procedures d'identifïcation peuvent être effectuer après l'analyse des hydrocarbones. Plus important, la technique permet l'analyse d'un seul specimen au lieu de plusieurs comme precedament.

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References

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Keywords

Variation in the cuticular hydrocarbons of larvae of Anopheles gambiae and A. arabiensis

  • Greg I. Anyanwu (a1), Angela Phillips (a1) and David H. Molyneux (a1)

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