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Characterisation of Midgut Antigens of Hyalomma anatolicum anatolicum Koch, 1844 (Acari: Ixodidae)

Published online by Cambridge University Press:  19 September 2011

Rajender Kumar
Affiliation:
National Research Centre on Camel, Jorbeer, P.B. 07, Bikaner-334001, Rajasthan, India
D. P. Banerjee
Affiliation:
Faculty of Veterinary and Animal Sciences, W.B.U.A.F.S., Kolkata-700037, India
P. P. Sengupta
Affiliation:
National Research Centre on Equines, Hisar-125001, Haryana, India
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Abstract

—The sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of gut supernate antigen (GS Ag) derived from partly fed Hyalomma anatolicum anatolicum Koch, 1844 (Acari: Ixodidae) adult females revealed 26 discrete potypeptide bands of molecular weight ranging from 25–208 kDa. Out of these, seven bandswere of major polypeptides with molecular weights of between 25.2 and 185.8 kDa. On immunoblotting with antisera raised in rabbits against gut supernate (crude extract), eight immunogenic polypeptides with molecular weights of between 51.7 and 185.8 were identified. The implications of these findings for the potential development of anti-tick vaccine are discussed.

Résumé

—Une électrophorèse en gel de polyacrylamide de dodecyl sulfate de sodium (SDS), réalisée avec des antigènes provenant d'extrait d'intestin de femelles adultes de Hyalomma anatolicum anatolicum Koch, 1844 (Acari: Ixodidae) partiellement nourries, révèle 26 bandes de polypeptides de poids moléculaires compris entre 25–208 kDa. Sept autres bandes de polypeptides majeurs de poids moléculaires compris entre 25,2 et 185,8 ont également été révélées. Par immunoprécipitation avec de l'antisérum obtenu de lapin à partir du surnageant d'intestin (extrait brut), huit polypeptides immunogéniques de poids moléculaires compris entre 51,8 et 185,8 ont été identifies. Les consequences de ces résultats pour la mise au point d'un vaccin anti-tique sont discutées.

Type
Research Articles
Copyright
Copyright © ICIPE 2002

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References

REFERENCES

Ackerman, S., Floyd, M. and Sonenshine, D.E. (1980) Artificial immunity to Dermacentor variabilis (Acari: Ixodidae). Vaccination using tick antigen. J. Med. Entomol. 17, 391397.CrossRefGoogle Scholar
Banerjee, D.P., Kumar, R., Kumar, S. and Sengupta, P.P. (2002) Immunization of crossbred cattle (Bos taunts × Bos indicus) with fractionated midgut antigen against Hyalomma anatolicum anatolicum tick. Trop. Anim. Hlth. Prod. (In press).Google Scholar
Brown, S.J., Shapiro, S.Z. and Askenase, P.W. (1984) Characterization of tick antigens inducing host immune resistance. I. Immunization of guinea pigs with Amblyomma americanum salivary gland extracts and identification of an important salivary gland antigen with guinea pig anti-tick antibodies. J. Immunol. 133, 33193325.CrossRefGoogle ScholarPubMed
Cruz-Vazquez, C., Garcia-Vazquez, , Quintero-Martinez, M. T., Miranda-Miranda, E., Rosario-Cruz, and Hernandez-Ortiz, R. (1996) Identification of gut antigens from female Boophilus microplus ticks. Parasitologia al Dia 20, 125129.Google Scholar
Jongejan, F., Pegram, R.G., Zivkovic, D., Hensen, E.J., Mwase, E.T., Thielemans, M.J.C., Coose, A., Niewold, T. A., Said, A.E. and Uilenberg, G. (1989) Monitoring of naturally acquired and artificially induced immunity to Amblyomma variegatum and Rhipicephalns appendiculatus ticks under field and laboratory conditions. Exp. Appl. Acarol. 7, 181199.CrossRefGoogle ScholarPubMed
Kaiser, M.N. and Hoogstraal, H. (1964) The Hyalomma ticks (Ixodoidea: Ixodidae) of Pakistan, India and Ceylon, with keys to subgenera and species. Acarologia 6, 257286.Google Scholar
Kumar, R. and Banerjee, D.P. (2001) Immunization of rabbits with midgut antigens against Hyalomma anatolicum anatolicum tick, p. 35. Abstracts of the 18th International Conference of World Association for the Advancement of Veterinary Parasitology, Stresa, Italy, Aug. 26–30, 2001Google Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680685.CrossRefGoogle ScholarPubMed
Lehninger, A.L., Nelson, D.L. and Cox, M. M. (1993) An introduction to proteins, pp. 134160. In Principles of Biochemistry. CBS Publishers, New Delhi.Google Scholar
Nolan, J. (1990) Acaricide resistance in single and multihost ticks and strategies for control. Parasitologia 32, 145153.Google ScholarPubMed
Nolan, J., Wilson, J.I., Green, R.E. and Bird, R.E. (1989) Synthetic pyrethroid resistance in field samples of the cattle tick (Boophilus microplus). Aust. Vet. J. 66, 179182.CrossRefGoogle ScholarPubMed
Opdebeeck, J.P., Wong, J.Y.M., Jackson, L. A. and Dobson, C. (1988a) Vaccines to protect Hereford cattle against the cattle tick, Boophilus microplus. Immunology 63, 363367.Google ScholarPubMed
Opdebeeck, J.P., Wong, J.Y.M., Jackson, L.A. and Dobson, C. (1988b) Hereford cattle immunised and protected against Boophilus microplus with soluble and membrane associated antigens from the midgut of ticks. Parasite Immunol. 10, 405410.CrossRefGoogle ScholarPubMed
Riding, G. A., Jarmey, J., McKenna, R. V., Pearson, R., Cobon, G.S., Willadsen, P. and Gough, J.M. (1994) A protective “concealed” antigen from Boophilus microplus: Purification, localization, and possible function. J. Immunol. 153, 51585166.CrossRefGoogle ScholarPubMed
Rodriguez, M., Massard, C. L., Da Fonseca, A. H., Ramos, N. E., Machado, H., Labarta, V. and De La Fuente, J. (1995a) Effect of vaccination with a recombinant Bm86 antigen preparation on natural infestations of Boophilus microplus in grazing, dairy, beef pure and cross-bred cattle in Brazil. Vaccine 13, 18041808.CrossRefGoogle ScholarPubMed
Rodriguez, M., Penichet, M.L., Mouris, A.E., Labarta, V., Larenzo Llauces, L., Rubiera, R., Cordoves Cv Sanchez, P.A., Ramos, E., Soto, A., Canales, M., Palenzuela, D., Triguero, A., Lleonart, R., Herrera, L. and De La Fuente, J. (1995b) Control of Boophilus microplus population in grazing cattle vaccinated with a recombinant Bm86 antigen preparation. Vet. Parasitol. 57, 339349.CrossRefGoogle ScholarPubMed
Towbin, H., Stachelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Nat. Acad. Sci., USA 76, 43504354.CrossRefGoogle ScholarPubMed
Varma, M.G.R., Heller-Haupt, A., Trinder, P.K.E. and Langi, A.O. (1990) Immunisation of guinea-pigs against Rhipicephahts appendiculatus adult ticks using homogenates from unfed immature ticks. Immunology 71, 133138.Google ScholarPubMed
Walker, A. R., Fletcher, J. D., McKeller, S. B., Bell, L. J. and Brown, C. G. D (1985) The maintenance and survival of Theileria annulata in colonies of Hyalomma anatolicum anatolicum. Ann. Trop. Med. Parasitol. 79, 199209.CrossRefGoogle ScholarPubMed
Wharton, R.H. (1976) Tick-borne livestock diseases and their vectors. 5. Acaricide resistance and alternative methods for tick control. Wld. Anim. Rev. 20, 815.Google Scholar
Wikel, S.K. (1981) The induction of host resistance to tick infestation with a salivary gland antigen. Am. J. Trop. Med. Hyg. 30, 284288.CrossRefGoogle ScholarPubMed
Wikel, S.K. (1988) Immunological control of haematophagous arthropod vectors: Utilization of novel antigens. Vet. Parasitol. 29, 235264.CrossRefGoogle Scholar
Willadsen, P. (1997) Novel vaccines for ectoparasites. Vet. Parasitol. 71, 209222.CrossRefGoogle ScholarPubMed
Willadsen, P., Bird, P., Cobon, G.S. and Hungerford, J. (1995) Commercialisation of a recombinant vaccine against Boophilus microplus. Parasitology (supplement) 110, S43–S50.CrossRefGoogle ScholarPubMed
Willadsen, P., Riding, G.A., McKenna, R.V., Kemp, D.H., Tellam, R.L., Nielsen, J.N., Lahnstein, J., Cobon, G.S. and Gough, J.M. (1989) Immunologic control of a parasite arthropod: Identification of a protective antigen from Boophilus microplus. J. Immunol. 143, 13461351.CrossRefGoogle ScholarPubMed
Wong, J.Y.M. and Opdebeeck, J.P. (1989) Protective efficacy of antigens solubilized from gut membranes of cattle tick, Boophilus microplus. Immunology 66, 149155.Google ScholarPubMed