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Acute toxicity and sublethal effects of the neonicotinoid imidacloprid on the fitness of Helicoverpa armigera (Lepidoptera: Noctuidae)

  • Salman Ahmad (a1), Mohammad Shafiq Ansari (a1) and Nadeem Ahmad (a1)


The fitness of Helicoverpa armigera (Hübner) was determined using demographic studies on the F1 generation that survived after exposure to four sublethal doses (LC50, LC30, LC20 and LC10) of imidacloprid. The effects were assessed on the surviving individuals emerged from sixth-instar larvae that had ingested imidacloprid-treated chickpea pods. Age-specific parameters were found to be highest with the commencement of age and gradually decreased with the progression of age in both the treated and non-treated groups. Survivorship was reduced to 37 days after exposure to the sublethal dose of LC50 when compared with 42 days in groups not exposed to the insecticide. The highest number (17%) of unhatched eggs was recorded in insects treated with the LC50 dose of imidacloprid, whereas 98% of the eggs hatched in the unexposed group. The potential fecundity of the F1 generation females was reduced to 330 eggs/female/generation when treated with the LC50 dose compared with that of the unexposed females. The intrinsic rate of increase was found to be least in insects exposed to the LC20 dose (0.0355 females/female/day) and highest (0.0470) in the unexposed group. It took 38.83 days for H. armigera to complete one generation in the unexposed population, while this was reduced to 33.94 days after exposure to the sublethal dose of LC50. The longevity of adults decreased when the larvae were exposed to the sublethal dose of imidacloprid. The developmental time of pre-pupae and pupae significantly decreased and was least when exposed to the highest sublethal dose of the insecticide. Thus, at sublethal doses, imidacloprid caused a significant reduction in the survival and fecundity as well as increased mortality of H. armigera in the subsequent generation after exposure to the insecticide. In conclusion, imidacloprid can be successfully incorporated into integrated pest management of H. armigera.


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Ahmad, S. and Ansari, M. S. (2013) Acute toxicity and sublethal effects of a pyrethroid (cypermethrin) on survival, development and fitness of Helicoverpa armigera. Archives of Phytopathology and Plant Protection 46, 17261739.
Ahmad, S., Ansari, M. S. and Moraiet, M. A. (2013) Demographic changes in Helicoverpa armigera after exposure to neemazal (1% EC azadirachtin). Crop Protection 50, 3036.
Ahmad, N., Ansari, M. S. and Nazrussalam, (2012) Effect of neemarin on life table indices of Plutella xylostella (L.). Crop Protection 38, 714.
Ahmed, S., Zia, K. and Shah, N. (2004) Validation of chemical control of gram pod borer, Helicoverpa armigera (Hub.) with new insecticides. International Journal Agriculture & Biology 6, 978980.
Ansari, M. S., Ahmad, T., Ali, H. and Gulrez, H. (2008) Effect of imidacloprid on development of Plutella xylostella. Annals of Plant Protection Sciences 16, 341346.
Birch, L. C. (1948) The intrinsic rate of natural increase of an insect population. Journal of Animal Ecology 17, 1526.
Bues, R., Bouvier, J. C. and Boudinhon, L. (2005) Insecticide resistance and mechanisms of resistance to selected strains of Helicoverpa armigera (Lepidoptera: Noctuidae) in the south of France. Crop Protection 24, 814820.
Deevey, E. S. (1947) Life tables for natural populations of animals. Quarterly Review of Biology 22, 283314.
Deling, M., Gordh, G. and Zalucki, M. P. (2000 a) Survival and development of Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) on neem (Azadirachta indica A. Juss) leaves. Australian Journal of Entomology 39, 208211.
Deling, M., Gordh, G. and Zalucki, M. P. (2000 b) Biological effects of azadirachtin on Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) fed on cotton and artificial diet. Australian Journal of Entomology 39, 301304.
Dixon, A. F. G. (1987) Parthenogenetic reproduction and the rate of increase in aphids, pp. 269287. In Aphids: Their Biology, Natural Enemies and Control (edited by Minks, A. K. and Harrewijn, P.). Elsevier, Amsterdam.
Elzen, G. W. (2001) Lethal and sublethal effects of insecticide residues on Orius insidiosus (Hemiptera: Anthocoridae) and Geocoris punctipes (Hemiptera: Lygaeidae). Journal of Economic Entomology 94, 5559.
Fitt, G. P. (1989) The ecology of Heliothis armigera in relation to agroecosystems. Annual Review of Entomology 34, 1752.
Fujiwara, Y., Takahashi, T., Yoshioka, T. and Nakasuji, F. (2002) Changes in egg size of the diamondback moth Plutella xylostella (Lepidoptera: Yponomeutidae) treated with fenvalerate at sublethal doses and viability of the eggs. Applied Entomology and Zoology 37, 103109.
Jacobson, A., Foster, R., Krupke, C., Hutchison, W., Pittendrigh, B. and Weinzierl, R. (2009) Resistance to pyrethroid insecticides in Helicoverpa zea (Lepidoptera: Noctuidae) in Indiana and Illinois. Journal of Economic Entomology 102, 22892295.
Lashkari, M. R., Sahragard, A. and Ghadamyari, M. (2007) Sub lethal effects of imidacloprid and pymetrozine on population growth parameters of cabbage aphid, Brevicoryne brassicae on rapeseed, Brassica napus L. Insect Science 14, 207212.
Luckey, T. D. (1968) Insecticide hormoligosis. Journal of Economic Entomology 61, 712.
McCaffery, A. R. (1998) Resistance to insecticides in heliothine Lepidoptera: a global view. Philosophical Transactions of the Royal Society London. B: Biological Sciences 353, 17351750.
Mahmoudvand, M., Abbasipour, H., Garjan, A. S. and Bandani, A. R. (2011) Sub lethal effects of indoxacarb on the diamondback moth, Plutella xylostella (L.) (Lepidoptera, Yponomeutidae). Applied Entomology and Zoology 46, 7580.
Minitab 11 Statistical Software. [Computer software]. State College, PA: Minitab, Inc. (
Nandihalli, B. S., Patil, B. V. and Hugar, P. (1992) Influence of synthetic pyrethroid usage on aphid resurgence in cotton. Karnataka Journal of Agricultural Sciences 5, 234237.
Pawar, C. S. (1998) Helicoverpa, a national problem which needs a national policy and commitment for its management. Pestology 22, 5159.
Pietrantonio, P. V., Junek, T. A., Parker, R., Mott, D., Siders, K., Troxclair, N., Vargas-Camplis, J. J., Westbrook, K. and Vassiliou, V. A. (2007) Detection and evolution of resistance to the pyrethroid cypermethrin in Helicoverpa zea (Lepidoptera: Noctuidae) populations in Texas. Environmental Entomology 36, 11741188.
Prakash, M. R., Ram, U. and Tariq, A. (2007) Evaluation of chickpea (Cicer arietinum L.) germplasm for resistance to gram pod borer, Helicoverpa armigera Hubner (Lepidoptera: Noctuidae). Journal of Entomological Research 31, 215218.
R Development Core Team (2010) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna. ISBN 3-17 900051-07-0, available at:
Rezaei, M., Talebi, K., Naveh, V. H. and Kavousi, A. (2007) Impacts of the pesticides imidacloprid, propargite, and pymetrozine on Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae): IOBC and life table assays. BioControl 52, 385398.
Saber, M., Parsaeyan, E., Vojoudi, S., Bagheri, M., Mehrvar, A. and Kamita, S. G. (2013) Acute toxicity and sublethal effects of methoxyfenozide and thiodicarb on survival, development and reproduction of Helicoverpa armigera (Lepidoptera: Noctuidae). Crop Protection 43, 1417.
Santos, T. M., Costa, N. P., Torres, A. L. and Boiça, A. L. Jr (2004) Effect of neem extract on the cotton aphid. Pesquisa Agropecuária Brasileira 39, 10711076.
Singh, S. K. and Yadav, D. K. (2009) Life table and biotic potential of Helicoverpa armigera (Hubner) on chickpea pods. Annals of Plant Protection Sciences 17, 9093.
Southwood, T. R. E. (1978) Ecological Methods with Particular Reference to the Study of Insect Populations 2nd edn.Chapman and Hall, London. 524 pp.
SPSS Inc. (1999) SPSS Base 10.0 for Windows User's Guide. SPSS Inc., Chicago IL.
Stark, J. D. and Banks, J. E. (2003) Population level effects of pesticides and other toxicants on arthropods. Annual Review of Entomology 48, 505519.
Stark, J. D., Banks, J. E. and Acheampong, S. (2004) Estimating susceptibility of biological control agents to pesticides: influence of life history strategies and population structure. Biological Control 29, 392398.
Stark, J. D., Vargas, R. and Banks, J. E. (2007) Incorporating ecologically relevant measures of pesticide effect for estimating the compatibility of pesticides and biocontrol agents. Journal of Economic Entomology 100, 10271032.
Stark, J. D. and Wennergren, U. (1995) Can population effects of pesticides be predicted from demographic toxicological studies? Journal of Economic Entomology 85, 10891096.
Sujana, G., Sharma, H. C. and Rao, D. M. (2008) Antixenosis and antibiosis components of resistance to pod borer Helicoverpa armigera in wild relatives of pigeonpea. International Journal of Tropical Insect Science 28, 191200.
Ugurlu, S. and Gurkan, M. O. (2007) Insecticide resistance in Helicoverpa armigera from cotton-growing areas in Turkey. Phytoparasitica 35, 376379.
Vojoudi, S., Saber, M., Hejazi, M. J. and Talaei-Hassanloui, R. (2011) Toxicity of chlorpyrifos, spinosad and abamectin on cotton bollworm, Helicoverpa armigera and their sublethal effects on fecundity and longevity. Bulletin of Insectology 64, 189193.
Walthall, W. K. and Stark, J. D. (1996) A comparison of acute mortality and population growth rate as endpoints of toxicological effects. Ecotoxicology and Environmental Safety 37, 4552.
Wang, D., Gong, P., Li, M., Qiu, X. and Wang, K. (2009) Sublethal effects of spinosad on survival, growth and reproduction of Helicoverpa armigera (Lepidoptera: Noctuidae). Pest Management Science 65, 223227.
Yin, X. H., Wu, Q. J., Li, X. F., Zhang, Y. J. and Xu, B. Y. (2009) Demographic changes in multigeneration Plutella xylostella (Lepidoptera: Plutellidae) after exposure to sublethal concentrations of spinosad. Journal of Economic Entomology 102, 357365.
Zalucki, M. P., Daglish, G., Firempong, S. and Twine, P. H. (1986) The biology and ecology of Heliothis armigera (Hübner) and H. punctigera Wallengren (Lepidoptera: Noctuidae) in Australia: what do we know? Australian Journal of Zoology 34, 779814.


Acute toxicity and sublethal effects of the neonicotinoid imidacloprid on the fitness of Helicoverpa armigera (Lepidoptera: Noctuidae)

  • Salman Ahmad (a1), Mohammad Shafiq Ansari (a1) and Nadeem Ahmad (a1)


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