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Leishmania in kenyan phlebotomine sandflies—IV: Artificial feeding and attempts to infect six species of laboratory-reared sandflies with Leishmania donovani

Published online by Cambridge University Press:  19 September 2011

J. B. Kaddu
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
M. J. Mutinga
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
M. P. Nyamori
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya

Abstract

Five (Sergentomyia antennatus, S. garnhami, S. schwetzi, S. ingrami and S. adleri) of the six species of sandflies fed on mammalian blood containing cultured Leishmania donovani promastigotes on day 0, were able to feed through cockerel skin membranes at 5.5, 10, 34.3, 45.7 and 95% feeding rates, respectively. Sergentomyia bedfordi was able to feed on mammalian blood only through a lizard skin membrane at 100% feeding rate. Promastigotes were found in 9.3, 25 and 37.5% of S. schwetzi, S. adleri and S. ingrami respectively, dissected on days 3–9.

The results open way for artificial feeding of Kenyan sandflies on mammalian blood through membranes, and indicate that it is necessary to select the type of membrane depending on sandfly species. The ability of L. donovani to develop in Kenyan sandflies is being investigated to experimentally establish and confirm the importance of various genera and species in the epidemiology of leishmaniasis.

Résumé

Des 6 espèces des mouches nourries sur le sang des mammifères contenant des promastigotes Leishmania donovani en culture au jour zéro, cinq des ces espèces (Sergentomyia antennatus, S. garnhami, S. schwetzi, S. ingrami et S. adleri) étaient capables de s'alimenter à travers les membranes de la peau avec un taux d'alimentation respectif de 5.5, 10, 34.3, 45.7 et 95%. Sergentomyia bedfordi était capable de s'alimenter sur le sang mammalien seulement qu'a travers la membrane de la peau du lézard à un taux de 100%. Les promastigotes étaient trouvés dans 9.3, 25 et 37.5% de S. schwetzi, S. adleri et S. ingrami respectivement, après dissection aux jours 3 et 9.

Les résultats ouvrent une voie artificielle d'alimentation sur le sang mammalien à travers les membranes et indiquent qu'il est nécessaire de sélectionner le type de membrane en concordance avec l'espèce des mouches.

L'habilité de L. donovani à se developper dans les mouches Kenyanes a été étudiée pour établir expérimentalement, et confirmer l'importance de divers generes et espèces dans l'épidemiologie de la leishmaniose.

Type
Research Articles
Copyright
Copyright © ICIPE 1986

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References

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Leishmania in kenyan phlebotomine sandflies—IV: Artificial feeding and attempts to infect six species of laboratory-reared sandflies with Leishmania donovani
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