Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-16T10:43:30.927Z Has data issue: false hasContentIssue false
  • English
  • Français

Plasmid Analysis of 26 Staphylococcal Species by a Rapid Microscale Technique

Published online by Cambridge University Press:  02 January 2015

Thomas Herchline  and
William Maher
Thomas Herchline*
Affiliation:
Division of Infectious Disease, Department of Internal Medicine, the Ohio State University College of Medicine, Columbus, Ohio
William Maher
Affiliation:
Division of Infectious Disease, Department of Internal Medicine, the Ohio State University College of Medicine, Columbus, Ohio
*
4881 Sugar Maple Dr, Wright-Patterson AFB, OH 45433
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective:

Evaluate a plasmid typing technique for a diverse group of staphylococci.

Design:

In vitro testing on known isolates.

Setting:

University hospital.

Intervention:

The plasmid content of 195 isolates representing 26 staphylococcal species was analyzed by agarose gel electrophoresis following lysostaphin-alkaline-SDS lysis, with and without acetone treatment.

Results:

Isolates yielded plasmid profiles with 0 to 7 extra-chromosomal bands (median, 1; 1.5); 171 (88%) had a profile with at least 1 band. Species with more than one isolate available for testing showed considerable diversity of plasmid profiles, except for Staphylococcus haemolyticus.

Conclusions:

The ease of the procedure and the diversity of plasmid profiles within each species examined suggests that plasmid profiling is an accessible and useful epidemiologic tool applicable to most staphylococcal species by epidemiologic units or clinical laboratories.

Type
Molecular Hospital Epidemiology
Information
Infection Control & Hospital Epidemiology , Volume 17 , Issue 10 , October 1996 , pp. 687 - 690
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Archer, GL, Vishniavsky, N, Stiver, HG. Plasmid pattern analysis of Staphylococcus epidermidis isolates from patients with prosthetic valve endocarditis. Infect Immun 1982;35:627–632.Google Scholar
2.Tang, J, Wu, SX. Plasmid profiles of multi-resistant Staphylococcus aureus at a children's hospital. Acta Paediatr Scand 1987;76:769–774.Google Scholar
3.Gillespie, MT, May, JW, Skurray, RA. Antibiotic susceptibilities and plasmid profiles of nosocomial methicillin-resistant Staphylococcus aureus: a retrospective study. J Med Microbiol 1984;17:295–310.Google Scholar
4.McGowan, JE Jr, Terry, PM, Huang, TSR, Houk, CL, Davies, J. Nosocomial infections with gentamicin-resistant Staphylococcus aureus: plasmid analysis as an epidemiologic tool. J Infect Dis 1979;140:864–872.Google Scholar
5.Collins, JK, Smith, JS, Kelly, MT. Comparison of phage typing, plasmid mapping, and antibiotic resistance patterns as epidemiologic markers in a nosocomial outbreak of methicillin-resistant Staphylococcus aureus infections. Diagn Microbiol Infect Dis 1984;2:233–245.Google Scholar
6.Ortqvist, A, Ransjo, U, Wretlind, B. Plasmid analysis as an epidemiological tool in neurosurgical infections with coagulase-negative staphylococci. Epidemiol Infect 1987;98:231–239.Google Scholar
7.Bialkowska-Hobrzanska, H, Jaskot, D, Hammerberg, O. Evaluation of restriction endonuclease fingerprinting of chromosomal DNA and plasmid profile analysis for characterization of multiresistant coagulase-negative staphylococci in bacteremic neonates. J Clin Microbiol 1990;28:269–275.Google Scholar
8.Hartstein, AI, Valvano, MA, Morthland, VH, Fuchs, PC, Potter, SA, Crosa, JH. Antimicrobic susceptibility and plasmid profile analysis as identity tests for multiple blood isolates of coagu-lase-negative staphylococci. J Clin Microbiol 1987;25:589–593.Google Scholar
9.Dunkle, LM, Sippel, CJ. Rapid microprocedure for extraction of plasmid DNA from Staphylococcus aureus. J Infect Dis 1984;149:921–923.Google Scholar
10.Holmes, DS, Quigley, M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 1981;114:193–197.Google Scholar
11.Birnboim, HC, Doly, J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979;7:1513–1523.Google Scholar
12.Kado, CI, Liu, ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981;145:1365–1373.Google Scholar
13.Takahashi, S, Nagano, Y. Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis. J Clin Microbiol 1984;20:608–613.Google Scholar
14.Wilson, CR, Totten, PA, Baldwin, JN. Rapid procedure for the detection of plasmids in Staphylococcus epidermidis. Appl Environ Microbiol 1978;36:368–374.Google Scholar
15.Kloos, WE, Lambe, JW. Staphylococcus. In: Balows, A, Hauser, WJ, Hermann, KL, Isenberg, HD, Shadomy, HJ, eds. Manual of Clinical Microbiology. 5th ed. Washington, DC: American Society for Microbiology; 1991:222–237.Google Scholar
16.Heath, LS, Sloan, GL, Heath, HE. A simple and generally applicable procedure for releasing DNA from bacterial cells. Appl Environ Microbiol 1986;51:1138–1140.Google Scholar
17.Kloos, WE, Schleifer, KH. Simplified scheme for routine identification of human Staphylococcus species. J Clin Microbiol 1975;1:82–88.Google Scholar
18.Maniatis, T, Fritsch, EF, Sanbrook, J. Isolation of bacteriophage 1 and plasmid DNA. In: Molecular Cloning. New York, NY: Cold Spring Harbor; 1982:90.Google Scholar
19.Kloos, WE, Orban, BS, Walker, DD. Plasmid composition of Staphylococcus species. Can J Microbiol 1981;27:271–278.Google Scholar
20.Dryden, MS, Talsania, H, McCann, M, Cookson, BD, Phillips, I. The epidemiology of ciprofloxacin resistance in coagulase-negative staphylococci in CAPD patients. Epidemiol Infect 1992;109:97–112.Google Scholar
21.Goetz, MB, Mulligan, ME, Kwok, R, O'Brien, H, Caballes, C, Garcia, JP. Management and epidemiologic analysis of an outbreak due to methicillin-resistant Staphylococcus aureus. Am J Med 1992;92:607–614.Google Scholar
22.Brown, AL, Stephenson, JR, Baker, LR, Tabaqchali, S. Epidemiology of CAPD-associated peritonitis caused by coagulase-negative staphylococci: comparison of strains from hands, abdominal Tenckhoff catheter exit site and peritoneal fluid. Nephrol Dial Transplant 1991;6:643–648.Google Scholar
23.Renaud, F, Etienne, J, Bertrand, A, et al.Molecular epidemiology of Staphylococcus haemolyticus strains isolated in an Albanian hospital. J Clin Microbiol 1991;29:1493–1497.Google Scholar
24.Tveten, Y, Kristiansen, BE, Ask, E, Jenkins, A, Hofstad, T. DNA fingerprinting of isolates of Staphylococcus aureus from new-borns and their contacts. J Clin Microbiol 1991;29:1100–1105.Google Scholar
25.Gianakopoulos, G, Herchline, T, Maher, W. Chlorhexidine bathing with antibiotic discontinuation as control measures for methicillin-resistant Staphylococcus aureus. In: Program and Abstracts of the 90th Annual Meeting of the American Society for Microbiology. Anaheim, CA; May 1990. Abstract C375;406.Google Scholar