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Nosocomial Outbreak of Pseudomonas aeruginosa Endophthalmitis

  • I. Mateos (a1), R. Valencia (a2), M. J. Torres (a3), A. Cantos (a1), M. Conde (a2) and J. Aznar (a1) (a3)...

Abstract

We describe an outbreak of nosocomial endophthalmitis due to a common source, which was determined to be trypan blue solution prepared in the hospital's pharmacy service. We assume that viable bacteria probably gained access to the trypan blue stock solution during cooling after autoclaving. The temporal cluster of Pseudomonas aeruginosa endophthalmitis was readily perceived on the basis of clinical and microbiological findings, and an exogenous source of contamination was unequivocally identified by means of DNA fingerprinting.

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Corresponding author

Servicio de Microbiología, Hospitales Universitarios Virgen del Rocío, Avda. Manuel Siurot, s/n 41013 Sevilla, Spain (javier.aznar.sspa@juntadeandalucia.es)

References

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1.Pier, GB, Ramphal, R. Pseudomonas aeruginosa. In: Mandell, GL, Bennett, JE, Dolin, R, eds. Mandell, Douglas and Bennett's Principles and Practice of Infectious Diseases. 6th ed. New York: Churchill Livingston; 2005:25872611.
2.Busbee, BG. Advances in knowledge and treatment: an update on endophthalmitis. Current Opin Ophthalmol 2004; 15:232237.
3.Callegan, MC, Engelbert, M, Parke, DW II, Jet, BD, Gilmore, MS. Bacterial endophthalmitis: epidemiology, therapeutics, and bacterium-host interactions. Clin Microbiol Rev 2002; 15:111124.
4.Cruciani, M, Malena, M, Amalfitano, G, Monti, P, Bonomi, L. Molecular epidemiology in a cluster of cases of postoperative Pseudomonas aeruginosa endophthalmitis. Clin Infect Dis 1998; 26:330333.
5.Hanscom, TA. Postoperative endophthalmitis. Clin Infect Dis 2004; 38:542546.
6.Taban, M, Behrens, A, Newcomb, RL, Nobe, MY, Saedi, G, Sweet, PM, et al. Acute endophtalmitis following cataract surgery: a systematic review of the literature. Arch Ophthalmol 2005; 123:613620.
7.Swaddiwudhipong, W, Linlawan, P, Prasantong, R, Kiphati, R, Wongwat-charapaiboon, P. A report of an outbreak of postoperative endophthalmitis. J Med Assoc Thai 2000; 83:902907.
8.Martin-Lozano, D, Cisneros, JM, Becerril, B, et al. Comparison of a repetitive extragenic palindromic sequence-based PCR method and clinical and microbiological methods for determining strain sources in cases of nosocomial Acinetobacter baumannii bacteriemia. J Clin Microbiol 2002; 40:45714575.
9.Woods, CR, Versalovic, J, Koeuth, T, Lupski, JR. Whole-cell repetitive element sequence–based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates. J Clin Microbiol 1993; 31:19271931.
10.Laureano, JS, Corroneo, MT. Crystalline lens capsule staining with trypan blue. J Cataract Refract Surg 2004; 30:20462049.
11.Saini, JS, Jain, AK, Sukhija, J, Gupta, P, Saroha, V. Anterior and posterior capsulorhexis in pediatric cataract surgery with or without trypan blue dye: randomized prospective clinical study. J Cataract Refract Surg 2003; 29:17321737.
12.Morel, PC, Roubi, N, Talon, DR, Betrand, X. Contamination of trypan blue with Burkholderia cepacia in a cornea bank. Infect Control Hosp Epidemiol 2003; 24:198201.
13.Doit, C, Simon, AM, Ferroni, A, et al. Outbreak of Bukholderia cepacia bacteraemia in a pediatric hospital due to contamination of lipid emulsion stoppers. J Clin Microbiol 2004; 42:22272230.

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Nosocomial Outbreak of Pseudomonas aeruginosa Endophthalmitis

  • I. Mateos (a1), R. Valencia (a2), M. J. Torres (a3), A. Cantos (a1), M. Conde (a2) and J. Aznar (a1) (a3)...

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