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A New Synergized Glutaraldehyde-Phenate Sterilizing Solution and Concentrated Disinfectant

  • Eddie D. Leach (a1)


Research leading to the development of Sporicidin, a new (synergized glutaraldehyde-phenate) sterilant and concentrated disinfectant, is reported. Microbial evaluation and shelf life testing of the new formulation was done using AOAC tests—Use-Dilution, Sporicidal, Tuberculocidal—and the E.P.A. Virucidal Test. Full-strength Sporicidin sterilizes (is sporicidal) in 6% hours at both 20C and 25C, and disinfects in 1-2 minutes. When diluted with 15 parts of tap water, Sporicidin is tuberculocidal, virucidal, and germicidal in 10 minutes, qualifying as a hospital disinfectant that will not “yellow” the hands or skin. The shelf life of activated Sporidicin, full strength or diluted 1 in 16, is 30 days. According to the data presented, which were the basis of E.P.A. registrations, Sporicidin is: (a) the fastest glutaraldehyde sterilant at room temperature, and (b) the only glutaraldehyde product that can be diluted below 2% and still qualify as a hospital disinfectant.


Corresponding author

Milligan College, Milligan College, TN 37682


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1.Block, SS. Disinfection, Sterilization and Preservation. (Ed 2). Philadelphia, Lea & Febiger, 1977.
2.Borick, PM, Pepper, RE. The spore problem. In: Benarde, MA (ed). Disinfection. New York, Marcel Dekker, 1970.
3.Spaulding, EH. Chemical disinfection in the hospital. J Hosp Res 1964;9:525.
4.Borick, PM (ed). Chemical Disinfection. Stroudsburg, PA, Dowden, Hutchinson & Ross, 1973.
5.Adair, FW, Geftic, SG, Gelzer, J. Resistance of Pseudomonas to quaternary ammonium compounds. I. Growth in benzalkonium chloride solution. Appl Microbiol 1969;18:299302.
6.Lee, JC, Fialkow, PJ. Benzalkonium chloride—source of hospital infection with gram-negative bacteria. JAMA 1961;177(10):708710.
7.Hirsch, JG. The resistance of tubercle bacilli to the bactericidal action of benzalkonium chloride (Zepharin). Am Rev Tuberc 1954;70:312319.
8.Mehta, P. Disinfection of medical and surgical materials. Am J Hosp Pharm 1971;28:454456.
9.Borick, PM. Chemical sterilizers (Chemosterilizers). In: Advances in Applied Microbiology 1968;10:302.
10.Editorial. Formaldehyde toxicity. Lancet 1979;2(8143):620621.
11.Pepper, RE, Chandler, VL. Sporicidal activity of alkaline alcoholic saturated dialdehyde solutions. Appl Microbiol 1963;11:384388.
12.Stonehill, AA, Krop, S, Borick, PM. Buffered glutaraldehyde, a new chemical sterilizing solution. Am J Hosp Pharm 1963;20:458465.
13.Borick, PM, Dondershine, FH, Chandler, VL. Alkalinized glutaraldehyde, a new antimicrobial agent. J Pharm Sci 1964;53:12731275.
14.Snyder, RW, Cheatle, EL. Alkaline glutaraldehyde, an effective disinfectant. Am J Hosp Pharm 1965;22:321327.
15.Pepper, RE, Lieberman, ER. Dialdehyde alcoholic sporicidal composition. U.S. Patent No. 3,016,328, 1962.
16.Rasmussen, KE, Albrechtsen, J. Glutaraldehyde. The influence of pH, temperature, and buffering on the polymerization rate. Histochem 1974;38:1926.
17.Miner, NA, McDowell, JW, Willcockson, GWet al.Antimicrobial and other properties of a new stabilized alkaline glutaraldehyde disinfectant/sterilizer. Am J Hosp Pharm 1977;34:376382.
18.Boucher, RMG. Advances in sterilization techniques, state of the art and recent breakthroughs. Am J Hosp Pharm 1972;29:661672.
19.Boucher, RMG. Potentiated acid 1,5 pentanedial solution—a new chemical sterilizing and disinfecting agent. Am J Hosp Pharm 1974;31:546557.
20.Pepper, RE. Comparison of the activities and stabilities of alkaline glutaraldehyde sterilizing solutions. Infect Control 1980;1:9092.
21.Schattner, RI. Buffered phenol-glutaraldehyde sterilizing compositions. U.S. Patent No. 4,103,001, 1978.
22.Horowitz, W. Official Methods of Analysis of the Association of Official Analytical Chemists. (12th ed). Washington, D.C., Association of Official Analytical Chemists, 1975.
23. Federal Register 10, No. 123, 25 June 1975, p. 26836.
24.Prince, H. Antiviral studies with aged Sporicidin. Report No. 10602, Reference 35-146-55, Gibraltar Biological Laboratories, Inc., Fairfield, NJ. Sept 14, 1979.
25.Meltzer, N, Henkin, H. Glutaraldehyde: A new preservative for cosmetics. Sixth Congress of International Federation of Societies of Cosmetic Chemists 1970;11:833850.
26.Lyon, TC. Allergic contact dermatitis due to Cidex, report of a case. Oral Surg, Oral Med, Oral Path 1971;32:895898.
27.Council on Dental Therapeutics. Council announces classification of additional products. 1978; JADA 97:857859.
28.Council on Dental Therapeutics. Council accepts dilution claims for Sporicidin. 1980; JADA 100:918.

A New Synergized Glutaraldehyde-Phenate Sterilizing Solution and Concentrated Disinfectant

  • Eddie D. Leach (a1)


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