Hostname: page-component-7479d7b7d-k7p5g Total loading time: 0 Render date: 2024-07-11T01:27:13.885Z Has data issue: false hasContentIssue false
  • English
  • Français

Monitoring Rotavirus Environmental Contamination in a Pediatric Unit Using Polymerase Chain Reaction

Published online by Cambridge University Press:  02 January 2015

Hervé Soule ,
Odile Genoulaz ,
Bénédicte Gratacap-Cavallier ,
Marie Reine Mallaret ,
Patrice Morand ,
Patrice François ,
Dominique Luu Duc Bin ,
Agnés Charvier ,
Cécile Bost-Bru  and
Jean-Marie Seigneurin
Hervé Soule*
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
Odile Genoulaz
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
Bénédicte Gratacap-Cavallier
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
Marie Reine Mallaret
Affiliation:
Unité d'Hygiene Hospitalière, CHU Grenoble, CH Annecy, France
Patrice Morand
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
Patrice François
Affiliation:
Département de Pédiatric, CHU Grenoble, CH Annecy, France
Dominique Luu Duc Bin
Affiliation:
Unité d'Hygiene Hospitalière, CHU Grenoble, CH Annecy, France
Agnés Charvier
Affiliation:
Service des Maladies Infectieuses, CH Annecy, France
Cécile Bost-Bru
Affiliation:
Département de Pédiatric, CHU Grenoble, CH Annecy, France
Jean-Marie Seigneurin
Affiliation:
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, CHU Grenoble, CH Annecy, France
*
Laboratoire de Virologie, CHU Grenoble, BP217, 38043 Grenoble Cedex 9, France
Get access Rights & Permissions [Opens in a new window]

Abstract

Rotavirus environmental contamination in a pediatric unit was investigated. Surfaces were swabbed, then viruses eluted, ultracentrifuged, and detected by polymerase chain reaction (PCR) amplification. Of 55 samples, 25 (46%) tested positive. Rotavirus RNA was more prevalent on surfaces in direct contact with children (thermometers and play mats) than on other environmental surfaces (washbasins, door handles, etc). PCR has proved useful for monitoring rotavirus environmental contamination.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 20 , Issue 6 , June 1999 , pp. 432 - 434
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Keswick, BH, Pickering, LK, DuPont, HL, Woodward, WE. Prevalence of rotavirus in children in day care centers. J Pediatr 1983;103:85–86.Google Scholar
2. Brown, DWG, Campbell, L, Tomkins, DS, Hambling, MH. School outbreak of gastroenteritis due to atypical rotavirus. Lancet 1989;2:737–738.Google Scholar
3. Nakata, S, Adachi, N, Ukac, S, Kogawa, K, Numata, K, Urasawa, S, et al. Outbreaks of nosocomial rotavirus gastro-enteritis in a paediatric ward. Eur J Pediatr 1996;155:954–958.Google Scholar
4. Kilgore, PE, Unicomb, LE, Gentsch, JR, Albert, MJ, McElroy, CA, Glass, RI. Neonatal rotavirus infection in Bangladesh: strain characterization and risk factors for nosocomial infection. Pediatr Infect Dis J 1996;15:672–677.Google Scholar
5. Gaggero, A, Avendano, LF, Fernandez, J, Spencer, E. Nosocomial transmission of rotavirus from patients admitted with diarrhea. J Clin Microbiol 1992;30:3294–3297.Google Scholar
6. Sattar, SA, Lloyd-Evans, N, Springthorpe, VS, Nair, RC. Institutional outbreaks of rotavirus diarrhoea: potential role of fomites and environmental surfaces as vehicles for virus transmission. J Hyg (Land) 1986;96:277–289.CrossRefGoogle ScholarPubMed
7. Ward, RL, Bernstein, DI, Young, EC, Sherwood, JR, Knowlton, DR, Schiff, GM. Human rotavirus studies in volunteers: determination of infectious dose and serological response to infection. J Infect Dis 1986;154:871–880.Google Scholar
8. Ansari, SA, Springthorpe, VS, Sattar, SA. Survival and vehicular spread of human rotaviruses: possible relation to seasonality of outbreaks. Rev Infect Dis 1991;13:448–461.Google Scholar
9. Wilde, J, Van, R, Pickering, L, Eiden, J, Yolken, R. Detection of rotaviruses in the day care environment by reverse transcriptase polymerase chain reaction. J Infect Dis 1992;166:507–511.Google Scholar
10. Boom, R, Sol, CJA, Salimans, MMM, Jansen, CL, Wertheim-Van Dillen, PME, Van der Noordaa, J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990;28:495–503.Google Scholar
11. Butz, AM, Fosarelli, P, Dick, J, Cusack, T, Yolken, R. Prevalence of rotavirus on high-risk fomites in day care facilities. Pediatrics 1993;92:202–205.Google Scholar
12. Shaw, RD, Hempson, SJ, Mackow, ER. Rotavirus diarrhea is caused by nonreplicating viral particles. J Virol 1995;69:5946–5950.Google Scholar
13. Ansari, SA, Sattar, SA, Springthorpe, VS, Wells, GA, Tostowaryk, W. In vivo protocol for testing efficacy of hand-washing agents against viruses and bacteria: experiments with rotavirus and Escherichia coli . Appl Environ Microbiol 1989;2:199–205.Google Scholar