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Investigation of an Apparent Cluster of Klebsiella pneumoniae Bacteremias Using Random Amplified Polymorphic DNA Analysis

Published online by Cambridge University Press:  02 January 2015

James C. Hurley ,
Edward G. Russell ,
Glenys Harrington  and
W. John Spicer
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Abstract

A cluster of bacteremia episodes with Klebsiella pneumoniae was noted in patients in a hematology-oncology ward during a 3-week period. Random amplified polymorphic DNA (RAPD) analysis, a novel technique for generating chromosomal fingerprints from bacterial isolates, was used as an aid to the epidemiological investigation of this cluster. For each of the two patients from whom multiple isolates had been obtained, identical RAPD patterns were observed in the serial isolates, even for a patient where the isolates had different biotypes. Isolates from different patients gave distinct patterns. Random amplified polymorphic DNA was found to be a useful typing technique for this cluster of K pneumonia bacteremias.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 17 , Issue 11 , November 1996 , pp. 743 - 745
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1996

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References

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