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Inactivation of Human Immunodeficiency Virus by a Medical Waste Disposal Process Using Chlorine Dioxide

  • R. Wesley Farr (a1) and Cheryl Walton (a1)

Abstract

Objective:

To study the ability of a medical waste disposal process using chlorine dioxide to inactivate human immunodeficiency virus type 1 (HIV 1).

Design:

Stock HIV-1 (HTLV-IIIB strain) was treated with chlorine dioxide under the following settings: cell culture medium alone, culture medium with 25% blood, culture medium with medical supplies treated by the Condor machine (Winfield Environmental Corp., Escondido, CA). MT-2 cells in 96-well tissue culture plates were inoculated with serial tenfold dilutions of treated and untreated HIV-1. Cytopathic effect was read on day five, and the TCID50 (50% tissue culture infectious dose) was calculated.

Results:

Treatment of HIV-1 with chlorine dioxide in culture medium alone resulted in a 5.25 log10 reduction in TCID50. Treatment of HIV-1 with chlorine dioxide in the presence of 25% blood caused a 6.25 log10 reduction in HIV-1 infectivity Treatment of HIV-1 with chlorine dioxide in the presence of medical supplies treated in the Condor machine resulted in a 4.75 log10 reduction in HIV infectivity.

Conclusions:

Chlorine dioxide inactivated HIV-1 in vitro. Chlorine dioxide inactivated HIV-1 in the presence of blood and in the presence of medical supplies under conditions that simulated the conditions existing in the Condor machine.

Copyright

Corresponding author

Section of Infectious Diseases, Department of Medicine, PO Box 9163, West Virginia University, Morgantown, WV 26506-9163

References

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1.Rutala, WA, Mayhall, CG. Medical waste. Infect Control Hosp Epidemiol 1992;13:3848.
2.Karpiak, J, Pugliese, G. Medical waste-declining options in the 90s. Am J Infect Control 1991;19:815.
3.Keene, JH. Medical waste: a minimal hazard. Infect Control Hosp Epidemiol 1991;12:682685.
4.Sattar, SA, Springthorpe, VS. Survival and disinfectant inactivation of the human immunodeficiency virus: a critical review. Reviews of Infectious Diseases 1991;13:430437.
5.Resnick, L, Veren, K, Salahuddin, SZ, et al. Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments. JAMA 1986;255:18871891.
6.Martin, LS, McDougal, S, Loskoski, SL. Disinfection and inactivation of the human T lymphottopic virus type III/lymphadenopathy-associated virus. J Infect Dis 1985;152:400403.
7.Sarin, PS, Scheer, DI, Kross, RD. Inactivation of human T-cell lymphotropic retrovirus (HTLV-III) by LD. N Engl J Med 1985;313:1416.
8.Chambers, RF, Terada, M, Kufe, D, et al. Shaking HIV-1 infected cells indicates novel behavior of MN strain. AIDS Res Hum Retroviruses 1991;7:459463.
9.Harada, S, Koyanagi, Y, Yamamoto, N. Infection of HTL-VIII/LAV in HTLVI-carrying cells MT-2 and MT-4 and application in plaque assay. Science 1985;229:563566.
10.Karber, G. Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Archiv Fur Experimentelle Pathologie Und Pharmakologie 1931;162:480483.
11.Hilfenhaus, J, Gregersen, JP, Muller, H, et al. Inactivation of retroviruses in biologicals manufactured for human use. Dev Biol Stand 1991;75:159169.
12.Prince, DL, Prince, RN, Prince, HN. Inactivation of human immunodeficiency virus type 1 and herpes simplex type 2 by commercial hospital disinfectants. Chemical Times and Trends 1990;13:13-16, 54.
13.Daschner, FD. Chemical disinfection of medical waste-a totally wrong approach. Infect Control Hosp Epidemiol 1993;14:64. Letter.

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Inactivation of Human Immunodeficiency Virus by a Medical Waste Disposal Process Using Chlorine Dioxide

  • R. Wesley Farr (a1) and Cheryl Walton (a1)

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