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Epidemiology of Acinetobacter baumannii in a University Hospital in Turkey

  • Halis Akalin (a1), Cüneyt Özakin (a1) and Suna Gedikoğlu (a1)

Abstract

Objective.

Molecular epidemiologic surveillance of Acinetobacter baumannii by polymerase chain reaction-randomly amplified polymorphic DNA analysis in a university hospital for 3 consecutive study periods.

Results.

Twelve different Acinetobacter baumannii genotypes (A-L) were detected. Although only 2 genotypes were detected during the first period and genotype A appeared to be the most common genotype, genotype D was included in these genotypes during the second study period. Genotype A completely disappeared during the third period. Although the presence of genotype C and the genotype D continued during the third period, 9 new genotypes were detected during this period. Genotype A appeared to be the most common genotype in the hospital (detected in 19 different clinics). The distribution of genotypes in clinical samples correlated with patient traffic between them. Some genotypes were found in both clinical and environmental samples. Seventeen different antibiotypes were detected, according to antibiotic susceptibility profiles.

Conclusions.

Environmental contamination, airborne transmission, patient transfer, and cross-contamination play important roles in epidemics caused by A. baumannii in our hospital. The distribution of genotypes can change over time, so antibiotyping is not appropriate for the epidemiological analysis of A. baumanii infection.

Copyright

Corresponding author

Uludağ University, School of Medicine, Department of Microbiology and Infectious Diseases, 16059, Bursa, Turkey (halis@uludag.edu.tr)

References

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1.Fagon, JY, Chastre, J, Hance, AJ, Montravers, P, Novara, A, Gibert, C. Nosocomial pneumonia in ventilated patients: a cohort study evaluating attributable mortality and hospital stay. Am J Med 1993; 94:281288.
2.Wisplinghoff, H, Edmond, MB, Pfaller, MA, Jones, RN, Wenzel, RP, Seifert, H. Nosocomial bloodstream infections caused by Acinetobacter species in the United States Hospitals: clinical features, molecular epidemiology, and antimicrobial susceptibility. Clin Infect Dis 2000; 31:690697.
3.Poutanen, SM, Tompkins, LS. Molecular methods in nosocomial epidemiology. In: Wenzel, RP, ed. Prevention and Control of Nosocomial Infections, ed. 4. Philadelphia: Lippincott, Williams & Wilkins; 2003:481499.
4.Grundmann, HJ, Towner, KJ, Dijkshoorn, L, et al. Multicenter study using standardized protocols and reagents for evaluation of reproducibility of PCR-based fingerprinting of Acinetobacter spp. J Clin Microbiol 1997; 35:30713077.
5.Graser, Y, Klare, I, Halle, E, et al. Epidemiological study of an Acinetobacter baumannii outbreak by using polymerase chain reaction fingerprinting. J Clin Microbiol 1993; 31:24172420.
6.Tankovic, J, Legrand, P, De Gatines, G, Chemineau, V, Brun-Buisson, C, Duval, J. Characterization of a hospital outbreak of imipenem-resistant Acinetobacter baumannii by phenotypic and genotypic typing methods. J Clin Microbiol 1994; 32:26772681.
7.Zarrilli, R, Crispino, M, Bagattini, M, et al. Molecular epidemiology of sequential outbreaks of Acinetobacter baumannii in an intensive care unit shows the emergence of carbapenem resistance. J Clin Microbiol 2004; 42:946953.
8.Struelens, MJ, Carlier, E, Maes, N, Serruys, E, Quint, WGV, van Belkum, A. Nosocomial colonization and infection with multiresistant Acinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR fingerprinting. J Hosp Infect 1993; 25:1532.
9.Manikal, VM, Landman, D, Saurina, G, Oydna, E, Lai, H, Quale, J. Endemic carbapenem-resistant Acinetobacter species in Brooklyn, New York: citywide prevalence, interinstitutional spread, and relation to antibiotic usage. Clin Infect Dis 2000; 31:101106.
10.Landman, D, Quale, JM, Mayorga, D, et al. Citywide clonal outbreak of multiresistant Acinetobacter baumannii and Pseudomonas aeruginosa in Brooklyn, NY. Arch Intern Med 2002; 162:15151520.
11.Houang, ETS, Chu, YW, Leung, CM, et al. Epidemiology and infection control implications of Acinetobacter spp. in Hong Kong. J Clin Microbiol 2001;39:228234.
12.D'Agata, EM, Thaver, V, Schaffner, W. An outbreak of Acinetobacter baumannii: the importance of cross-transmission. Infect Control Hosp Epidemiol 2000;21:588591.
13.Bergogne-Berezin, E, Towner, JK. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 1996; 9:148165.
14.Dijkshoorn, L, Aucken, H, Gerner-Smidt, P, et al. Comparison of outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods. J Clin Microbiol 1996; 34:15191525.
15.Martin-Lozano, D, Cisneros, JM, Beceril, B, et al. Comparison of repetitive extragenic palindromic sequence-PCR method and clinical and microbiological methods for determining strain sources in cases of nosocomial Acinetobacter baumannii bacteremia. J Clin Microbiol 2002; 40:45714575.
16.Belkum, A, Struelens, M, Visser, A, Verbrugh, H, Tibayrenc, M. Role of genomic typing in taxonomy, evolutionary genetics, and microbial epidemiology. Clin Microbiol Rev 2001; 14:547560.
17.Gouby, A, Carles-Nurit, MJ, Bouziges, N, Bourg, G, Mesnard, R, Bouvet, PJM. Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinatobacter baumannii. J Clin Microbiol 1992; 30:15881591.
18.Seifert, H, Gerner-Smidt, P. Comparison of ribotyping and pulsed-field gel electrophoresis for molecular typing of Acinetobacter isolates. J Clin Microbiol 1995; 33:14021407.
19.Siau, H, Yuen, KY, Wong, SSY, Ho, PL, Luk, WK. The epidemiology of acinetobacter infections in Hong Kong. J Med Microbiol 1996; 44:340347.
20.Grundmann, H, Schneider, CH, Tichy, HV, et al. Automated laser fluorescence analysis of randomly amplified polymorphic DNA: a rapid method for investigating nosocomial transmission of Acinetobacter baumannii. J Med Microbiol 1995; 43:446451.
21.Ayan, M, Durmaz, R, Aktaş, E, Durmaz, B. Bacteriological, clinical and epidemiological characteristics of hospital-acquired Acinatobacter baumannii infection in a teaching hospital. J Hosp Infect 2003; 54:3945.
22.Bou, G, Cervero, G, Dominguez, MA, Quereda, C, Martinez-Beltran, J. PCR-based DNA fingerprinting (REP-PCR, AP-PCR) and pulsed-field gel electrophoresis characterization of a nosocomial outbreak caused by imipenem- and meropenem-resistant Acinetobacter baumannii. Clin Microbiol Infect 2000; 6:635643.

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