Skip to main content Accessibility help

Efficacy of Combination of N-acetylcysteine, Gentamicin, and Amphotericin B for Prevention of Microbial Colonization of Ventricular Assist Devices

  • Maria D. Hernandez (a1), Mohammad D. Mansouri (a1) (a2), Saima Aslam (a3) (a4) (a1), Barry Zeluff (a4) and Rabih O. Darouiche (a3) (a1) (a2)...


We assessed the in vitro antimicrobial activity and the in vivo efficacy of dipping ventricular assist devices in a combination of N-acetylcysteine, gentamicin, and amphotericin B (NAC/G/A). Ventricular assist devices dipped in NAC/G/A exhibited broad-spectrum antimicrobial activity in vitro and were less likely than undipped devices to become colonized with Staphylococcus aureus in a rabbit model.


Corresponding author

Center for Prostheses Infection, 1333 Moursund Avenue, Suite A221, Houston, TX 77030 (


Hide All
1.Gordon, RJ, Quagliarello, B, Lowy, FD. Ventricular assist device-related infections. Lancet Infect Dis 2006;6:426437.
2.Perez-Giraldo, C, Rodriguez-Benito, A, Moran, FJ, Hurtado, C, Blanco, MT, Gomez-Garcia, AC. Influence of N-acetylcysteine on the formation of biofilm by Staphylococcus epidermidis. J Antimicrob Chemother 1997;39:643646.
3.Mansouri, MD, Darouiche, RO. In vitro antimicrobial activity of N-acetylcysteine against bacteria colonising central venous catheters. Int J Antimicrob Agents 2007;29:474476.
4.Sherertz, RJ, Forman, DM, Solomon, DD. Efficacy of dicloxacillin-coated Polyurethane catheters in preventing subcutaneous Staphylococcus aureus infection in mice. Antimicrob Agents Chemother 1989;33:11741178.
5.Clinical and Laboratory Standards Institute (CLSI). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. 7th ed. Wayne, PA: CLSI; 2006:M07A7.
6.Yu, VL, Weber, R, Raoult, D, eds. Antimicrobial Therapy and Vaccines. 2nd ed. New York: Apple Trees Productions; 2003.
7.Darouiche, RO, Mansouri, MD. In vitro activity and in vivo efficacy of antimicrobial-coated vascular grafts. Ann Vase Surg 2004;18:497501.
8.Sherertz, RJ, Carruth, WA, Hampton, AA, Byron, MP, Solomon, DD. Efficacy of antibiotic-coated catheters in preventing subcutaneous Staphylococcus aureus infection in rabbits. J Infect Dis 1993;167:98106.
9.Raad, I, Darouiche, R, Dupuis, J, et al., Texas Medical Center Catheter Study Group. Central venous catheters coated with minocycline and rifampin for the prevention of catheter-related colonization and bloodstream infections: a randomized, double-blind trial. Ann Intern Med 1997;127:267274.
10.Aslam, S, Trautner, BW, Ramanathan, V, Darouiche, RO. Combination of tigecycline and N-acetylcysteine reduces biofilm-embedded bacteria on vascular catheters. Antimicrob Agents Chemother 2007;51:15561558.


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed