Skip to main content Accessibility help

Comparing the Bioburden Measured by Adenosine Triphosphate (ATP) Luminescence Technology to Contact Plate–Based Microbiologic Sampling to Assess the Cleanliness of the Patient Care Environment

  • Elizabeth Salsgiver (a1), Daniel Bernstein (a1), Matthew S. Simon (a2), William Greendyke (a3), Haomiao Jia (a3), Amy Robertson (a1) (a2), Selma Salter (a1) (a2), Audrey N. Schuetz (a1) (a2) (a4), Lisa Saiman (a2) (a3), E. Yoko Furuya (a2) (a3) and David P. Calfee (a1) (a2)...


The correlation between ATP concentration and bacterial burden in the patient care environment was assessed. These findings suggest that a correlation exists between ATP concentration and bacterial burden, and they generally support ATP technology manufacturer-recommended cutoff values. Despite relatively modest discriminative ability, this technology may serve as a useful proxy for cleanliness.

Infect Control Hosp Epidemiol 2018;39:622–624


Corresponding author

Address correspondence to David P. Calfee, MD, MS, Weill Cornell Medicine, 525 East 68th Street, Box 265, New York, NY 10065 (


Hide All

PREVIOUS PRESENTATION. An abstract summarizing the results of this study was presented as a poster at IDWeek 2016 on October 27, 2016, in New Orleans, Louisiana (Abstract 269).



Hide All
1. Weber, DJ, Rutala, WA, Miller, MB, Huslage, K, Sickbert-Bennett, E. Role of hospital surfaces in the transmission of emerging health care-associated pathogens: norovirus, Clostridium difficile, and Acinetobacter species. Am J Infect Control 2010;38(5 Suppl 1):S25S33.
2. Eckstein, BC, Adams, DA, Eckstein, EC, et al. Reduction of Clostridium difficile and vancomycin-resistant Enterococcus contamination of environmental surfaces after an intervention to improve cleaning methods. BMC Infect Dis 2007;7:61.
3. Toolkit: options for evaluating environmental cleaning. Centers for Disease Control and Prevention website. Published 2010. Accessed August 15, 2017.
4. Huang, YS, Chen, YC, Chen, ML, et al. Comparing visual inspection, aerobic colony counts, and adenosine triphosphate bioluminescence assay for evaluating surface cleanliness at a medical center. Am J Infect Control 2015;43:882886.
5. Dancer, SJ. How do we assess hospital cleaning? A proposal for microbiological standards for surface hygiene in hospitals. J Hosp Infect 2004;56:1015.
6. Ho, YH, Wang, LS, Jiang, HL, et al. Use of a sampling area-adjusted adenosine triphosphate bioluminescence assay based on digital image quantification to assess the cleanliness of hospital surfaces. Int J Environ Res Public Health 2016;13(6). doi: 10.3390/ijerph13060576.
7. Smith, PW, Beam, E, Sayles, H, et al. Impact of adenosine triphosphate detection and feedback on hospital room cleaning. Infect Control Hosp Epidemiol 2014;35:564569.
8. Brown, R, Eder, AR, Thompson, KM. Do surface and cleaning chemistries interfere with ATP measurement systems for monitoring patient room hygiene? J Hosp Infect 2010;74:193195.


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed