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Application of Multilocus Enzyme Electrophoresis to Epidemiologic Investigations of Xanthomonas Maltophilia

Published online by Cambridge University Press:  21 June 2016

Barbara Schable ,
Margarita E. Villarino ,
Martin S. Favero  and
J. Michael Miller
Barbara Schable*
Affiliation:
Nosocomial Infections Laboratory Branch, Hospital Infections Program, Centers for Disease Control, Public Health Service, U.S. Dept. of Health and Human Services, Atlanta, Georgia
Margarita E. Villarino
Affiliation:
Nosocomial Infections Laboratory Branch, Hospital Infections Program, Centers for Disease Control, Public Health Service, U.S. Dept. of Health and Human Services, Atlanta, Georgia
Martin S. Favero
Affiliation:
Nosocomial Infections Laboratory Branch, Hospital Infections Program, Centers for Disease Control, Public Health Service, U.S. Dept. of Health and Human Services, Atlanta, Georgia
J. Michael Miller
Affiliation:
Nosocomial Infections Laboratory Branch, Hospital Infections Program, Centers for Disease Control, Public Health Service, U.S. Dept. of Health and Human Services, Atlanta, Georgia
*
Centers for Disease Control, 1600 Clifton Road, Mailstop C01, Atlanta, GA 30333
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Abstract

Objective:

To test the utility of a newly developed multilocus enzyme electrophoresis typing method for Xanthomonas maltophilia.

Design:

Isolates were first screened by slide agglutination, which served as the standard to characterize the outbreak strains. All isolates were then subjected to multilocus enzyme elec-trophoresis and the results analyzed based on epidemiological data.

Setting:

This outbreak occurred in a shock-trauma intensive care unit of a large general community hospital.

Patients:

Patients admitted to the shock-trauma intensive care unit who had X maltophilia isolated from any site > 24 hours after admission met the case definition. Specimens from patients who fit the case definition were characterized, as were specimens from other patients that were used as controls for nonoutbreak isolates. Environmental samples were also evaluated for X maltophilia.

Results:

Most of the 64 isolates received during this outbreak were serotype 10, and when they were subjected to multilocus enzyme electro-phoresis, one electrophoretic type predominated and correlated to most outbreak isolates. Unrelated isolates of serotype 10 from other institutions all exhibited unique electrophoretic types.

Conclusion:

Application of multilocus enzyme electrophoresis to X maltophilia outbreaks is a valuable addition to the characterization of suspected outbreak strains.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 12 , Issue 3 , March 1991 , pp. 163 - 167
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1991

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