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An Epidemiologic Survey of Methicillin-Resistant Staphylococcus Aureus by Combined Use of Mec-HVR Genotyping and Toxin Genotyping in a University Hospital in Japan

Published online by Cambridge University Press:  02 January 2015

Junichiro Nishi*
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Masao Yoshinaga
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Hiroaki Miyanohara
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Motoshi Kawahara
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Masaharu Kawabata
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Toshiro Motoya
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Tetsuhiro Owaki
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Shigeru Oiso
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Masayuki Kawakami
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Shigeko Kamewari
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Yumiko Koyama
Affiliation:
Kagoshima University Hospital, Infection Control Team, Kagoshima University, Kagoshima, Japan
Naoko Wakimoto
Affiliation:
Department of Pediatrics, Faculty of Medicine, Kagoshima University, Kagoshima, Japan
Koichi Tokuda
Affiliation:
Department of Pediatrics, Faculty of Medicine, Kagoshima University, Kagoshima, Japan
Kunihiro Manago
Affiliation:
Department of Pediatrics, Faculty of Medicine, Kagoshima University, Kagoshima, Japan
Ikuro Maruyama
Affiliation:
The Committee of Infection Control, Kagoshima University, Kagoshima, Japan
*
Department of Pediatrics, Faculty of Medicine, Kagoshima University, Sakuragaoka 8-35-1, Kagoshima 890-8520, Japan

Abstract

Objective:

To evaluate the usefulness of an assay using two polymerase chain reaction-based genotyping methods in the practical surveillance of methicillin-resistant Staphylococcus aureus (MRSA).

Methods:

Nosocomial infection and colonization were surveyed monthly in a university hospital in Japan for 20 months. Genotyping with mec-HVR is based on the size of the mec-associated hypervariable region amplified by polymerase chain reaction. Toxin genotyping uses a multiplex polymerase chain reaction method to amplify eight staphylococcal toxin genes.

Results:

Eight hundred nine MRSA isolates were classified into 49 genotypes. We observed differing prevalences of genotypes for different hospital wards, and could rapidly demonstrate the similarity of genotype for outbreak isolates. The incidence of genotype D: SEC/TSST1 was significantly higher in isolates causing nosocomial infections (49.5%; 48 of 97) than in nasal isolates (31.4%; 54 of 172) (P = .004), suggesting that this genotype may represent the nosocomial strains.

Conclusion:

The combined use of these two genotyping methods resulted in improved discriminatory ability and should be further investigated.

Type
Original Articles
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2002

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References

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An Epidemiologic Survey of Methicillin-Resistant Staphylococcus Aureus by Combined Use of Mec-HVR Genotyping and Toxin Genotyping in a University Hospital in Japan
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