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Unequal crossing-over within the B duplication of Drosophila melanogaster: a molecular analysis

Published online by Cambridge University Press:  14 April 2009

Stuart I. Tsubota
Stuart I. Tsubota
Affiliation:
Department of Biology, University of Michigan, Ann Arbor, Michigan 49109, USA
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Summary

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The B mutation is associated with a tandem duplication of 16A1–16A7. It is unstable, mutating to wild type and to a more extreme form at a frequency of one in 1000 to 3000. The reversion to wild type is associated with the loss of one copy of the duplication, whereas the mutation to extreme B is associated with a triplication of the region. The instability of B has been attributed to unequal crossing-over between the two copies of the duplication. Recent molecular data show that there is a transposable element, B104, between the two copies of the duplication and support the hypothesis that this element generated the duplication via a recombination event. These data suggest that unequal crossing-over within the duplication may not be the cause of the instability of B. Instead, the instability may be caused by a recombination event involving the B104 element. This issue was addressed using probes for the DNA on either side of the B104 element at the B breakpoint. All of the data indicate that the B104 element is not involved in the instability of B and support the original unequal crossing-over model.

Type
Research Article
Information
Genetics Research , Volume 57 , Issue 2 , April 1991 , pp. 105 - 111
Copyright
Copyright © Cambridge University Press 1991

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