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Synchrony of division in mass cultures of Paramecium tetraurelia by a selective density-labelling technique*

Published online by Cambridge University Press:  14 April 2009

Karl Aufderheide
Karl Aufderheide
Affiliation:
Department of Zoology, Jordan Hall, Indiana University, Bloomington IN 47401, U.S.A.
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Summary

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A technique has been developed for achieving synchrony of division by selection for immediate pre- and post-fission cells of Paramecium tetraurelia. Cell populations are fed granular tantalum, followed by density-gradient centrifugation. Dividing cells, which do not feed, are separated from feeding interphase cells. The selected cells are division-synchronous for at least two generations when resuspended in fresh culture fluid. This technique provides a rapid means of obtaining large numbers of synchronously dividing paramecia for cell-cycle studies and has general application for selective enrichment of non-feeding sub-populations of cells.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 171 - 177
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Dippell, R. V. (1955). A temporary stain for Paramecium and other ciliate protozoa. Stain Technology 30, 69–71.CrossRefGoogle ScholarPubMed
Dryl, S. & Preer, J. R. (1967). The possible mechanism of resistance of Paramecium aurelia to kappa toxin from killer stock 7, syngen 2 during autogamy, conjugation and cell division. Journal of Protozoology 14 (Suppl.), 33–34.Google Scholar
James, T. W. (1966). Cell synchrony, a prologue to discovery. In Cell Synchrony (ed. Cameron, I. L. and Padilla, G. M.), pp. 1–13. New York: Academic Press.Google Scholar
Jones, W. R. (1976). Oral morphogenesis during asexual reproduction in Paramecium tetraurelia. Genetical Research 27, 187–204.CrossRefGoogle ScholarPubMed
Orias, E. & Pollock, N. A. (1975). Heat-sensitive development of the phagocytic organelle in a Tetrahymena mutant. Experimental Cell Research 90, 345–356.CrossRefGoogle Scholar
Preer, J. R. (1975). The hereditary symbionts of Paramecium aurelia. Society for Experimental Biology 29, 125–144.Google Scholar
Sonneborn, T. M. (1970). Methods in Paramecium research. In Methods in Cell Physiology, vol. 4, pp. 241–339. New York: Academic Press.Google Scholar
Sonneborn, T. M. (1974). Paramecium aurelia. In Handbook of Genetics, vol. 2 (ed. King, R. C.), pp. 469–594. New York: Plenum Press.CrossRefGoogle Scholar
Sonneborn, T. M. (1975). The Paramecium aurelia complex of fourteen sibling species. Transactions of the American Microscopic Society 94, 155–178.CrossRefGoogle Scholar
Wolfe, J. (1973). Differential labeling and gradient centrifugation in Tetrahymena. Experimental Cell Research 77, 232–238.CrossRefGoogle ScholarPubMed