Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-19T22:40:08.025Z Has data issue: false hasContentIssue false
  • English
  • Français

A study on the mate-killer toxin by microinjection in Paramecium

Published online by Cambridge University Press:  14 April 2009

Sadaaki Koizumi  and
Sumiko Kobayashi
Sadaaki Koizumi
Affiliation:
Research Institute for Science Education, Miyagi College of Education, Sendai, Japan
Sumiko Kobayashi
Affiliation:
Research Institute for Science Education, Miyagi College of Education, Sendai, Japan
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The killing action by mu toxin, which is contained in the cytoplasm of stock 540, Paramecium primaurelia, was demonstrated against the various stocks of paramecia by means of microinjection. Most of the toxin is present in the soluble fraction of the host cytoplasm. The toxin was precipitated by ammonium sulphate at 50–80% saturation, and was almost completely inactivated by incubation at 60 °C for 30 min. Pre-autogamous paramecia were more sensitive than post-autogamous ones to the toxin. Paramecia which bear endosymbionts were generally resistant to the mate-killer toxin.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 179 - 185
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Beale, G. H. & Jurand, A. (1960). Structure of the mate-killer (mu) particles in Paramecium aurelia, stock 540. Journal of General Microbiology 23, 243–252.CrossRefGoogle ScholarPubMed
Beale, G. H. & Jurand, A. (1966). Three different types of mate-killer (mu) particle in Paramecium aurelia (syngen 1). Journal of Cell Science 1, 31–34.Google Scholar
Gibson, I. & Beale, G. H. (1961). Genic basis of the mate-killer trait in Paramecium aurelia, stock 540. Genetical Research 2, 82–91.CrossRefGoogle Scholar
Jurand, A., Rudman, B. M. & Preer, J. R. Jr (1971). Prelethal effects of killing action by stock 7 of Paramecium aurelia. Journal of Experimental Zoology 177, 365–387.CrossRefGoogle ScholarPubMed
Koizumi, S. (1974). Microinjection and transfer of cytoplasm in Paramecium: Experiments on the transfer of kappa particles into cells at different stages. Experimental Cell Research 88, 74–78.Google Scholar
Levine, M. (1953). The divers mate-killers of Paramecium aurelia, variety 8: their interrelations and genetic basis. Genetics 38, 561–578.CrossRefGoogle Scholar
Preer, J. R. Jr & Preer, L. B. (1967). Virus-like bodies in killer paramecia. Proceedings of the National Academy of Science, U.S.A. 58, 1774–1781.CrossRefGoogle ScholarPubMed
Preer, J. R. Jr, Preer, L. B. & Jurand, A. (1974). Kappa and other endosymbionts in Paramecium aurelia. Bacteriological Review 38, 113–163.Google Scholar
Preer, L. B., Jurand, A., Preer, J. R. Jr & Rudman, B. M. (1972). The classes of kappa in Paramecium aurelia. Journal of Cell Science 11, 581–600.CrossRefGoogle ScholarPubMed
Preer, L. B. & Preer, J. R. Jr (1964). Killing activity from lysed particles of Paramecium. Genetical Research 5, 230–239.Google Scholar
Siegel, R. W. (1953). A genetic analysis of the mate-killer trait in Paramecium aurelia, variety 8. Genetics 38, 550–560.CrossRefGoogle ScholarPubMed
Siegel, R. W. (1954). Mate-killing in Paramecium aurelia, variety 8. Physiological Zoology 27, 89–100.CrossRefGoogle Scholar
Sonneborn, T. M. (1938). Mating types in P. aurelia: divers conditions for mating in different stocks; occurrence, number and interrelations of the types. Proceedings of the American Philosophical Society 79, 411–434.Google Scholar
Sonneborn, T. M. (1950). Methods in the general biology and genetics of Paramecium aurelia. Journal of Experimental Zoology 113, 87–143.CrossRefGoogle Scholar
Sonneborn, T. M. (1970). Methods in Paramecium research. Methods in Cell Physiology 4, 241–339.Google Scholar
Williams, J. (1971). The growth in vitro of killer particles from Paramecium aurelia and the axenic culture of this protozoan, Journal of General Microbiology 68, 253–262.Google Scholar