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Restriction map and α-amylase activity variation among Drosophila mutation accumulation lines

  • H. Tachida (a1), K. Harada (a2), C. H. Langley (a3), C. F. Aquadro (a3), T. Yamazaki (a2), C. C. Cockerham (a1) and T. Mukai (a2)...

Summary

The specific activities of α-amylase were measured for two sets of mutation accumulation lines, each set having originated from a different lethal-carrying second chromosome and SM1(Cy) chromosome and having been maintained by a balanced lethal system for about 300 generations. Significant variation was found to have accumulated among lines of both sets. Because of dysgenic crosses in the early generations of mutation accumulation, insertions or deletions of transposable elements in the Amy gene region were suspected of being the cause of this variation. In order to test this possibility, the structural changes in the 14 kb region of these chromosomes that includes the structural genes for α-amylase were investigated by restriction map analysis. We found that most part of the activity variation is due to replacements of a chromosomal region of SM1(Cy), including the structural genes for α-amylase, by the corresponding regions of the lethal chromosomes. One line also contained an insertion in this region but this line has an intermediate activity value. Thus, insertions of transposable elements into the Amy gene region were not found to be responsible for the new variation observed in α-amylase activity. If we remove those lines with structural changes from the analysis, the genetic variance of α-amylase specific activity among lines becomes non-significant in both sets of chromosomes.

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Copyright

Corresponding author

* Corresponding author: Present address: National Institute of Genetics, Mishima, Shizuoka-ken 411. Japan.

References

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Abraham, I. & Doane, W. W. (1978). Genetic regulation of tissue-specific expression of Amylase structural genes in Drosophila melanogaster. Proceedings of National Academy of Sciences, U.S.A. 75, 44464450.
Bender, W., Spierer, P. & Hogness, D. S. (1983). Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. Journal of Molecular Biology 168, 1733.
Chovnick, A., Gelbart, W. & McCarron, M. (1977). Organization of the rosy locus in Drosophila melanogaster. Cell 11, 110.
Doane, W. W., Treat-Clemons, L. G., Buchberg, A. M., Gemmil, R. M., Levy, J. N., Hawley, S. A. & Paigen, K. (1983). Genetic mechanisms for tissue specific control of alpha amylase expression in Drosophila melanogaster. In Isozymes: Current Topics in Biological and Medical Research, vol. 9 (ed. Rattazi, M. C., Scandalios, J. G. and Whitt, G. S.), pp. 6390. New York: Alan R. Liss.
Finnegan, D. J. & Fawcett, D. H. (1986). Transposable elements in Drosophila melanogaster. Oxford Surveys on Eukaryotic Genes 3, 162.
Gemmil, R. M., Levy, J. N. & Doane, W. W. (1985). Molecular cloning of α-amylase genes from Drosophila melanogaster. I. Clone isolation by use of a mouse probe. Genetics 110, 299312.
Hilliker, A. J. & Chovnick, A. (1981). Further observations on intragenic recombination in Drosophila melanogaster. Genetical Research 38, 281296.
Hoorn, A. J. W. & Scharloo, W. (1978). The functional significance of amylase polymorphism in Drosophila melanogaster. I. Proteins of two amylase variants. Genetica 49, 173180.
Lamb, B. C. (1984). The properties of meiotic gene conversion important in its effects on evolution. Heredity 53, 113138.
Langley, C. H., Shrimpton, A. E., Yamazaki, T., Miyashita, N., Matsuo, Y. & Aquadro, C. H. (1988). Naturally occurring variation in the restriction map of the Amy region of Drosophila melanogaster. Genetics 119, 619629.
Laurie-Ahlberg, C. C. (1985). Genetic variation affecting the expression of enzyme-coding genes in Drosophila: an evolutionary perspective. In Isozymes: Current Topics in Biological and Medical Research, vol. 12 (ed. Rattazi, M. C., Scandalios, J. G. and Whitt, G. S.), pp. 3388. New York: Alan R. Liss.
Levy, J. N., Gemmil, R. M. & Doane, W. W. (1985). Molecular cloning of α-amylase genes from Drosophila melanogaster. II. Clone organization and verification. Genetics 110, 313324.
Lindsley, D. C. & Grell, E. H. (1968). Genetic Variations of Drosophila melanogaster. Publication 627. Washington, D.C.: Carnegie Institute.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265275.
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Publications, New York.
Maroni, G. & Laurie-Ahlberg, C. C. (1983). Genetic control of Adh expression in Drosophila melanogaster. Genetics 105, 921933.
Mukai, T. & Cockerham, C. C. (1977). Spontaneous mutation rates at enzyme loci in Drosophila melanogaster. Proceedings of the National Academy of Sciences, U.S.A. 74, 25142517.
Mukai, T. & Voelker, R. A. (1977). The genetic structure of natural populations of Drosophila melanogaster. XIII. Further studies on linkage disequilibrium. Genetics 86, 175185.
Mukai, T., Harada, K. & Yoshimaru, H. (1984). Spontaneous mutations modifying the activity of Alcohol Dehydrogenase (ADH) in Drosophila melanogaster. Genetics 106, 7384.
Rigby, P. W. J., Dieckman, M., Rhodes, C. & Berg, P. (1977). Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113, 237251.
Scobie, N. N. & Schaffer, H. E. (1982 a). A mutator factor in a strain of Drosophila melanogaster: identified by use of mutation, reversion rates and male recombination. Genetics 101, 417429.
Scobie, N. N. & Schaffer, H. E. (1982 b). The location of mutator factor in a strain of Drosophila melanogaster by assaying male recombination. Genetics 101, 405416.
Smith, G. & Summers, M. (1980). The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxy-methyl paper. Annals of Biochemistry 109, 123129.
Voelker, R. A., Schaffer, H. E. & Mukai, T. (1980). Spontaneous allozyme mutations in Drosophila melanogaster: rate of occurence and nature of mutants. Genetics 94, 961968.
Yamaguchi, O. & Mukai, T. (1974). Variation of spontaneous occurrence rates of chromosomal abberations in the second chromosome of Drosophila melanogaster. Genetics 78, 12091221.
Yamazaki, T. & Matsuo, M. (1984). Genetic analysis of natural populations of Drosophila melanogaster in Japan. III. Genetic variability of inducing factors of amylase and fitness. Genetics 108, 223235.

Restriction map and α-amylase activity variation among Drosophila mutation accumulation lines

  • H. Tachida (a1), K. Harada (a2), C. H. Langley (a3), C. F. Aquadro (a3), T. Yamazaki (a2), C. C. Cockerham (a1) and T. Mukai (a2)...

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