Hostname: page-component-848d4c4894-x5gtn Total loading time: 0 Render date: 2024-05-12T22:48:58.876Z Has data issue: false hasContentIssue false
  • English
  • Français

Organ-specific variability and inheritance of maize proteins revealed by two-dimensional electrophoresis

Published online by Cambridge University Press:  14 April 2009

A. Leonardi ,
C. Damerval  and
D. de Vienne
A. Leonardi*
Affiliation:
Laboratoire de Génétique des Systémes Végétaux, La Ferme du Moulon, 91190 Gif-sur-Yvette, France
C. Damerval
Affiliation:
Laboratoire de Génétique des Systémes Végétaux, La Ferme du Moulon, 91190 Gif-sur-Yvette, France
D. de Vienne
Affiliation:
Laboratoire de Génétique des Systémes Végétaux, La Ferme du Moulon, 91190 Gif-sur-Yvette, France
*
† Corresponding author.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), the genetic variation of proteins was investigated in three organs (mesocotyl, sheath and blade of second leaf) from two maize lines and their hybrids. Nine hundred and eighty-six spots were numbered over the three organs. One hundred and fifty-five polypeptides were found to be genetically variable, due to allelic polymorphism of structural genes and/or to polymorphism of any genetic elements controlling protein amounts. Of these 155 variants 12% clearly showed complete dominance effects in the hybrid patterns, which could reveal dominance effects in the regulation of the protein biosynthesis. Comparison of the three organs showed that (i) the level of variability between lines depended upon the organ, since it varied from 7·5% for the blade to 12·6% for the mesocotyl and 13·2% for the sheath; (ii) 68 polypeptides displayed different kinds of variation in different organs and (iii) in all cases but one the dominant inheritance was organ-specific.

Type
Research Article
Information
Genetics Research , Volume 52 , Issue 2 , October 1988 , pp. 97 - 103
Copyright
Copyright © Cambridge University Press 1988

References

Anderson, N. L., Nance, S. L., Tollaksen, S. L., Giere, F. A. & Anderson, N. G. (1985). Quantitative reproducibility of measurements from Coomassie blue-stained 2-D gels: analysis of mouse liver protein patterns and a comparison of Balb/C and C57 strains. Electrophoresis 6, 592–599.CrossRefGoogle Scholar
Damerval, C., de Vienne, D., Zivy, M. & Thieliement, H. (1986). Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis 7, 52–54.CrossRefGoogle Scholar
Damerval, C., Hébert, Y. & de Vienne, D. (1987). Is the polymorphism of protein amounts related to phenotypic variability? A comparison of two-dimensional electro-phoresis data with morphological traits in maize. Theoretical and Applied Genetics 74, 194–202.CrossRefGoogle Scholar
Damerval, C., Guilloux, M. Le, Blaisonneau, J. & de Vienne, D. (1987). A simplification of Heukeshoven and Dernick's silver staining of proteins. Electrophoresis 8, 158–159.CrossRefGoogle Scholar
Das, O. P. & Messing, J. W. (1987). Allelic variation and differential expression at the 27-kilodalton zein locus in maize. Molecular and Cellular Biology 7, 4490–4497.Google ScholarPubMed
Garrels, J. I. (1979). Two-dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines. Journal of Biological Chemistry 254, 7961–7977.CrossRefGoogle ScholarPubMed
Granier, F. & de Vienne, D. (1985). Silver staining of proteins: standardized procedure for two-dimensional gels bound to polyester sheets. Analytical Biochemistry 155, 45–50.CrossRefGoogle Scholar
King, J. J. & McDonald, J. F. (1987). Post-translational control of alcohol dehydrogenase levels in Drosophila melanogaster. Genetics 115, 693–699.CrossRefGoogle ScholarPubMed
Klose, J. (1982). Genetic variability of soluble proteins studied by two-dimensional electrophoresis on different inbred mouse strains and on different mouse organs. Journal of Molecular Evolution 18, 315–328.CrossRefGoogle ScholarPubMed
Kuhlemeier, C., Green, P. J. & Chua, N.-H. (1987). Regulation of gene expression in higher plants. Annual Review of Plant Physiology 38, 221–257.CrossRefGoogle Scholar
Leonardi, A., Damerval, C. & de Vienne, D. (1987). Inheritance of protein amounts: comparison of two-dimensional electrophoresis patterns of leaf sheaths of two maize lines (Zea mays L.) and their hybrids. Genetical Research 50, 1–5.CrossRefGoogle Scholar
McLellan, T. & Inouye, L. S. (1986). The sensitivity of isoelectric focusing and electrophoresis in the detection of sequence differences in proteins. Biochemical Genetics 24, 571–577.CrossRefGoogle ScholarPubMed
O'Farrell, P. (1975). High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4007–4021.CrossRefGoogle ScholarPubMed
Paigen, K. (1979). Acid hydrolases as models of genetic control. Annual Review of Genetics 13, 417–466.CrossRefGoogle ScholarPubMed