Hostname: page-component-848d4c4894-r5zm4 Total loading time: 0 Render date: 2024-07-03T08:10:49.622Z Has data issue: false hasContentIssue false
  • English
  • Français

A nuclear gene modifying instability of fertility restoration in cytoplasmic male sterile rice

Published online by Cambridge University Press:  14 April 2009

Yoshio Sano ,
Mitsugu Eiguchi ,
Hiro-Yuki Hirano  and
Masa-Aki Yamada
Yoshio Sano
Affiliation:
National Institute of Genetics, Mishima, 411Japan
Mitsugu Eiguchi
Affiliation:
National Institute of Genetics, Mishima, 411Japan
Hiro-Yuki Hirano
Affiliation:
National Institute of Genetics, Mishima, 411Japan
Masa-Aki Yamada
Affiliation:
National Institute of Genetics, Mishima, 411Japan

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The present study was carried out to examine the genetic mechanism responsible for reversions to fertile phenotype detected in cytoplasmic male-sterile plants of rice. The cms-bo cytoplasm of Chinsurah boro II gave rise to male-sterility in plants without a gametophytic restorer gene (Rf1). Taichung 65 (T65A) was known to be the maintainer which carries no restorer; however, Taichun 65 preserved in our laboratory (T65B) showed partial fertility (about 8% seed set) when crossed with the male-sterile plants. Unexpectedly, the seed fertility gradually increased with repeated selfings and almost fully fertile plants were obtained in the F6 generation. The cytoplasmic substitution lines revealed that reversions to fertile phenotype resulted from mutational events at the nuclear level. The genetic experiments indicated that the partial fertility observed in the F1 hybrid was controlled by a dominant gene, Ifr1, which was carried by T65B. The results obtained suggested that Ifr1 itself was associated with instability of fertility restoration in the presence of cms-bo cytoplasm since partially fertile plants carrying Ifr1 always showed a tendency for gradual increase in fertility in the later generations. The results are also discussed in relation to a rapid genetic change through intensified gametic selection combined with instability.

Type
Research Article
Information
Genetics Research , Volume 60 , Issue 3 , December 1992 , pp. 195 - 200
Copyright
Copyright © Cambridge University Press 1992

References

Duvick, D. N. (1965). Cytoplasmic pollen sterility in corn. Advances in Genetics 13, 1–56.CrossRefGoogle Scholar
Edwardson, J. R. (1970). Cytoplasmic male sterility. Botanical Review 36, 341–420.CrossRefGoogle Scholar
Escote-Carlson, L. J., Gabay-Laughnan, L. J. & Laughnan, J. R. (1988). Reorganization of mitochondrial genomes of cytoplasmic revertants in cms-S inbred line WF9 in maize. Theoretical and Applied Genetics 75, 659–667.CrossRefGoogle Scholar
Kadowaki, K., Ishige, T., Suzuki, S., Harada, K. & Shinjyo, C. (1986). Differences in the characteristics of mitochondrial DNA between normal and male-sterile cytoplasm of Japonica rice. Japanese Journal of Breeding 36, 333–339.Google Scholar
Laughnan, J. R. & Gabay, S. J. (1973). Mutations leading to nuclear restoration of fertility in 5 male-sterile cytoplasm in maize. Theoretical and Applied Genetics 43, 109–116.CrossRefGoogle Scholar
Laughnan, J. R. & Gabay-Laughnan, S. (1982). Nuclear control over reversions to male fertility in S male-sterile maize. In: Maize Biological Research (ed. Sheridan, W. F.), pp. 239–242. Grand Forks: University of North Dakota Press.Google Scholar
Laughnan, J. R. & Gabay-Laughnan, S. (1983). Cytoplasmic male sterility in maize. Annual Review of Genetics 17, 27–48.CrossRefGoogle ScholarPubMed
Nawa, S., Sano, Y., Yamada, M. A. & Fujii, T. (1987). Cloning of the plasmids in cytoplasmic male sterile rice and changes of organization of mitochondrial and nuclear DNA in cytoplasmic reversion. Japanese Journal of Genetics 62, 301–314.Google Scholar
Saleh, N. M., Mulligan, B. J., Cocking, E. C. & Gupta, H. S. (1989). Small mitochondrial DNA molecules of wild abortive cytoplasm in rice are not necessarily associated with CMS. Theoretical and Applied Genetics 77, 617–619.CrossRefGoogle Scholar
Shinjyo, C. (1969). Cytoplasmic genetic male sterility in cultivated rice, Oryza saliva L. II. The inheritance of male sterility. Japanese Journal of Genetics 44, 149–156.Google Scholar
Shinjyo, C. (1975). Genetical studies of cytoplasmic male sterility and fertility restoration in rice, Oryza sativa L. Bulletin of the College of Agriculture, University of the Ryukyus 22, 1–57.Google Scholar
Singh, A. & Laughnan, J. R. (1972). Instability of S malesterile cytoplasm in maize. Genetics 71, 607–620.CrossRefGoogle ScholarPubMed
Virmani, S. S. & Shinjyo, C. (1988). Current status of analysis and symbols for male-sterile cytoplasms and fertility-restoring genes. Rice Genetics Newsletter 5, 9–15.Google Scholar
Yamaguchi, H. & Kakiuchi, H. (1982). Electrophoretic analysis of mitochondrial DNA from normal and malesterile cytoplasm in rice. Japanese Journal of Genetics 58, 607–611.Google Scholar
Zhou, T. L., Shen, J. H. & Ye, F. C. (1983). A genetic analysis on the fertility of Hsien type hybrid rice with wild rice cytoplasm. Ada Agronomica Sinica 9, 241–247.Google Scholar