Skip to main content Accessibility help
×
Home

A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L.

  • KULJIT K. CHEEMA (a1), NAVJIT K. GREWAL (a1), YOGESH VIKAL (a1), RAJIV SHARMA (a1), JAGJEET S. LORE (a2), APARNA DAS (a1), DHARMINDER BHATIA (a1), RITU MAHAJAN (a1), VIKAS GUPTA (a1), TAJINDER S. BHARAJ (a2) and KULDEEP SINGH (a1)...

Summary

Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv oryzae (Xoo) is one of the major constraints to productivity in South-East Asia. The strategy of using major genes, singly or in combination, continues to be the most effective approach for BB management. Currently, more than two dozen genes have been designated but not all the known genes are effective against all the prevalent pathotypes. The challenge, therefore, is to continue to expand the gene pool of effective and potentially durable resistance genes. Wild species constitute an important reservoir of the resistance genes including BB. An accession of Oryza nivara (IRGC 81825) was found to be resistant to all the seven Xoo pathotypes prevalent in northern states of India. Inheritance and mapping of resistance in O. nivara was studied by using F2, BC2F2, BC3F1 and BC3F2 progenies of the cross involving Oryza sativa cv PR114 and the O. nivara acc. 81825 using the most virulent Xoo pathotype. Genetic analysis of the segregating progenies revealed that the BB resistance in O. nivara was conditioned by a single dominant gene. Bulked segregant analysis (BSA) of F2 population using 191 polymorphic SSR markers identified a ∼35 centiMorgans (cM) chromosomal region on 4L, bracketed by RM317 and RM562, to be associated with BB resistance. Screening of BC3F1 and BC2F2 progenies and their genotyping with more than 30 polymorphic SSR markers in the region, covering Bacterial artificial chromosome (BAC) clone OSJNBb0085C12, led to mapping of the resistance gene between the STS markers based on annotated genes LOC_Os04g53060 and LOC_Os04g53120, which is ∼38·4 kb. Since none of the known Xa genes, which are mapped on chromosome 4L, are effective against the Xoo pathotypes tested, the BB resistance gene identified and transferred from O. nivara is novel and is tentatively designated as Xa30(t). Homozygous resistant BC3F3 progenies with smallest introgression region have been identified.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L.
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L.
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L.
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author. Tel. +91-161-2401960 extn. 270. Fax. +91-161-2401444. e-mail: kuldeep35@yahoo.com

References

Hide All
Berloo, R. V. (1999). GGT: software for the display of graphical genotypes. Journal of Heredity 90, 328329.
Churchill, G. A. & Doerge, R. W. (1994). Empirical threshold values for quantitative trait mapping. Genetics 138, 963971.
Dai, L., Liu, X., Xiao, Y. & Wang, G. (2007). Recent advances in cloning and characterization of disease resistance genes in rice. Journal of Integrative Plant Biology 49, 112119.
Gnanamanickam, S. S., Priyadarisini, V. B., Narayanan, N. N., Vasudevan, P. & Kavitha, S. (1999). An overview of bacterial blight disease of rice and strategies for its management. Current Science 77, 14351444.
Gonzalez, C., Szurek, B., Manceau, C., Mathieu, T., Séré, Y. & Verdier, V. (2007). Molecular and pathotypic characterization of new Xanthomonas oryzae strains from West Africa. Molecular Plant-Microbe Interactions 20, 534546.
Gu, K., Tian, D., Yang, F., Wu, L., Sreekala, C., Wang, G. L. & Yin, Z. (2003). High-resolution genetic mapping of Xa27(t), a new bacterial blight resistance gene in rice, O. sativa L. Theoretical and Applied Genetics 108, 800807.
He, Q., Li, D., Zhu, Y., Tan, M., Zhang, D. & Lin, X. (2006). Fine mapping of Xa2, a bacterial blight resistance gene in rice. Molecular Breeding 17, 16.
Heuer, S. & Miézan, K. M. (2003). Assessing hybrid sterility in Oryza glaberrima×Oryza sativa hybrid progenies by PCR marker analysis and crossing with wide compatibility varieties. Theoretical and Applied Genetics 107, 902909.
IRGSP (2005). The map-based sequence of the rice genome. Nature 436, 793800.
Kauffman, H. E., Reddy, A. P. K., Hsieh, S. P. Y. & Merca, S. D. (1973). An improved technique for evaluating resistance to rice varieties of Xanthomonas oryzae. Plant Disease Reporter 57, 537541.
Kaur, R., Grewal, N., Das, A., Vikal, Y., Singh, J., Bharaj, T. S., Sidhu, J. S. & Singh, K. (2005). Inheritance of bacterial blight resistance in two accessions of wild rice, Oryza nivara. Rice Genetics Newsletter 22, 7882.
Khush, G. S. (2001). Green revolution: the way forward. Nature Review Genetics 2, 815822.
Khush, G. S., Bacalangco, E. & Ogawa, T. (1990). A new gene for resistance to bacterial blight from O. longistaminata. Rice Genetics Newsletter 7, 121122.
Kinoshita, T. & Takashi, M. (1991). The one hundredth report of genetic studies on rice plant – linkage studies and future prospects. Journal of Faculty of Agriculture, Hokaido University 65, 116.
Kosambi, D. D. (1944). The estimation of map distances from recombination values. Annals of Eugenics 12, 172175.
Lander, E. S., Green, P., Abrahamson, J., Barlow, A., Daly, M. J., Lincoln, S. E. & Newburg, L. (1987). MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174181.
Lee, B., Park, Y., Park, D., Kang, H., Kim, J., Song, E., Park, I., Yoon, U., Hahn, J., Koo, B., Lee, G., Kim, H., Park, H., Yoon, K., Kim, J., Jung, C., Koh, N., Seo, J. & Go, S. (2005). The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice. Nucleic Acids Research 33, 577586.
Lin, X. H., Zhang, D. P., Xie, Y. F., Gao, H. P. & Zhang, Q. (1996). Identifying and mapping a new gene for bacterial blight resistance in rice based on RFLP markers. Phytopathology 86, 11561159.
Lore, J. S. & Raina, G. L. (2005). Donors resistance to bacterial blight of rice in Punjab. Plant Disease Research 20, 40.
Lorieux, M., Ndjiondjop, M. N. & Ghesqui, A. (2000). A first interspecific Oryza sativa×Oryza glaberrima microsatellite based genetic linkage map. Theoretical and Applied Genetics 100, 593601.
Manly, K. F., Cudmore, R. H. Jr & Meer, J. M. (2001). Map Manager QTX, cross-platform software for genetic mapping. Mammal Genome 12, 930932.
McCouch, S. R., Teytelman, L., Xu, Y., Lobos, K. B., Clare, K., Walton, M., Fu, B., Maghirang, R., Li, Z., Xing, Y., Zhang, Q., Kono, I., Yano, M., Fjellstrom, R., DeClerck, G., Schneider, D., Cartinhour, S., Ware, D. & Stein, L. (2002). Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Research 9, 199207.
Mew, T., Leung, H., Savary, S., Vera, Cruz C. M. & Leach, J. E. (2004). Looking ahead in rice disease research and management. Critical Reviews of Plant Science 23, 103127.
Mew, T. W., Alvamz, A. M., Leach, J. E. & Swings, J. (1993). Focus on bacterial blight of rice. Plant Disease 77, 512.
Michelmore, R. W., Paran, I. & Kesseli, R. V. (1991). Identification of markers linked to disease resistance genes by bulked segregant analysis: rapid method to detect markers in specific genomic regions by using segregating population. Proceedings of the National Academy of Sciences of the USA 88, 98289832.
Nakagahra, M., Omura, M. T. & Iwata, N. (1972). Gametophyte genes and their loci on the eleventh linkage group of cultivated rice. Japanese Journal of Breeding 22, 305312.
Nino-Liu, D. O., Ronald, P. C. & Bogdanove, A. J. (2006). Xanthomonas oryzae pathovars: model pathogens of a model crop. Molecular Plant Pathology 7, 303324.
Ochiai, H., Inoue, Y., Takeya, M., Sasaki, A. & Kaku, H. (2005). Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity. Japanese Agriculture Research Quarterly 39, 275287.
Ogawa, T., Morinaka, T., Fujii, K. & Kimura, T. (1978). Inheritance of resistance of rice varieties Kogyoku and Java 14 to bacterial group V of Xanthomonas oryzae. Annals of Phytopathological Society of Japan 44, 137141.
Reddy, A. P. K., Mackenzie, D. R., Rouse, D. I. & Rao, A. V. (1979). Relationship of bacterial leaf blight Xanthomonas oryzae severity to grain yield of rice Oryza sativa. Phytopathology 69, 967969.
Sobrizal, Matsuzaki Y. & Yoshimura, A. (2002). Mapping of pollen semi-sterility gene, S28(t), on rice chromosome 4. Rice Genetics Newsletter 19, 8082.
Sun, X., Yang, Z., Wang, S. & Zhang, Q. (2003). Identification of a 47-kb DNA fragment containing Xa4, a locus for bacterial blight resistance in rice. Theoretical and Applied Genetics 106, 683687.
Tan, G. X., Ren, X., Weng, Q. M, Shi, Z. Y., Zhu, L. L. & He, G. C. (2004). Mapping of a new resistance gene to bacterial blight in rice line introgressed from Oryza officinalis. Yi Chuan Xue Bao 31, 724729.
Taura, S., Ogawa, T., Tabien, R. E., Khush, G. S., Yoshimura, A. & Omura, T. (1987). The specific reaction of Taichung Native 1 to Philippine race of bacterial blight and inheritance of resistance to race 5 (PX0112). Rice Genetics Newsletter 4, 101102.
Temnykh, S., DeClerck, G., Lukashova, A., Lipovich, L., Cartinhour, S. & McCouch, S. (2001). Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential. Genome Research 11, 14411452.
Vikal, Y., Goel, R. K., Singh, R. & Singh, K. (2004). Molecular characterization of some bacterial blight resistant rice germplasm lines using STS markers linked to Xa4, xa5, xa13 and Xa21. Plant Disease Research 19, 2530.
Vikal, Y., Das, A., Patra, B., Goel, R. K., Sidhu, J. S. & Singh, K. (2007). Identification of new sources of bacterial blight (Xanthomonas oryzae pv. oryzae) resistance in wild Oryza species and O. glaberrima. Plant Genetic Resources: Characterization and Utilization 5, 108112.
Voorrips, R. E. (2002). MapChart: software for the graphical presentation of linkage maps and QTLs. Journal of Heredity 93, 7778.
Wu, J., Mizuno, H., Hayashi-Tsugane, M., Ito, Y., Chiden, Y., Fujisawa, M., Katagiri, S., Saji, S., Yoshiki, S., Karasawa, W., Yoshihara, R., Hayashi, A., Kobayashi, H., Ito, K., Hamada, M., Okamoto, M., Ikeno, M., Ichikawa, Y., Katayose, Y., Yano, M., Matsumoto, T. & Sasaki, T. (2003). Physical maps and recombination frequency of six rice chromosomes. Plant Journal 36, 720730.
Xu, Y., Zhu, L., Xiao, J., Huang, N. & McCouch, S. (1997). Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid, and recombinant inbred populations in rice (Oryza sativa L.). Molecular and General Genetics 253, 535545.
Yang, Z., Sun, X., Wang, S. & Zhang, Q. (2003). Genetic and physical mapping of a new gene for bacterial blight resistance in rice. Theoretical and Applied Genetics 106, 14671472.
Yoshimura, S., Umehara, Y., Kurata, N., Nagamura, Y., Sasaki, T., Minobe, Y. & Iwata, N. (1996). Identification of a YAC clone carrying the Xa-1 allele, a bacterial blight resistance gene in rice. Theoretical and Applied Genetics 93, 117122.
Yoshimura, S., Yamanouchi, U., Katayose, Y., Tok, S., Wang, Z., Kono, I., Kurata, N., Yano, M., Iwata, N. & Sasaki, T. (1998). Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation. Proceedings of the National Academy of Sciences of the USA 95, 16631668.
Zhang, Q., Lin, S. C., Zhao, B. Y., Wang, C. L., Yang, W. C., Zhao, Y. L., Li, D. Y., Chen, C. D. & Zhu, L. H. C. (1998). Identification and tagging of a new gene for bacterial blight (Xanthomonas oryzae pv oryzae) from Oryza rufipogon. Rice Genetics Newsletter 15, 138142.

A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L.

  • KULJIT K. CHEEMA (a1), NAVJIT K. GREWAL (a1), YOGESH VIKAL (a1), RAJIV SHARMA (a1), JAGJEET S. LORE (a2), APARNA DAS (a1), DHARMINDER BHATIA (a1), RITU MAHAJAN (a1), VIKAS GUPTA (a1), TAJINDER S. BHARAJ (a2) and KULDEEP SINGH (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed