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Mouse endogenous X-linked genes do not show lineage-specific delayed inactivation during development

Published online by Cambridge University Press:  14 April 2009

Jeanne M. Lebon ,
Patrick P. L. Tam ,
Judith Singer-Sam ,
Arthur D. Riggs  and
Seong-Seng Tan
Jeanne M. Lebon
Affiliation:
Department of Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010-0269, USA
Patrick P. L. Tam
Affiliation:
Embryology Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, NSW 2145, Australia
Judith Singer-Sam*
Affiliation:
Department of Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010-0269, USA
Arthur D. Riggs
Affiliation:
Department of Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010-0269, USA
Seong-Seng Tan
Affiliation:
Embryology Laboratory, Department of Anatomy and Cell Biology, University of Melbourne, Parkville, VIC 3052, Australia
*
* Corresponding author.
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X chromosome inactivation (XCI) has been assumed to be complete in all cells of female mouse embryos at about 6 d post coitum (dpc). However, a recent study on β-galactosidase expression of an X-linked lacZ transgene suggests that XCI is probably not complete several days after this time in some lineages. To help resolve this issue, we analysed XCI in embryos which carry the T(X;16)16H (Searle's) translocation and are heterozygous at the X-linked Hprt and Pgk-1 genes. The quantitative RT-PCR single nucleotide primer extension (SNuPE) assay was used to measure Hprt and Pgk-1 allele-specific transcripts in embryos 9·5 dpc. No transcripts from the normal X chromosome were found in any of the tissues tested, indicating that inactivation was complete for these endogenous genes.

Type
Short Paper
Information
Genetics Research , Volume 65 , Issue 3 , June 1995 , pp. 223 - 227
Copyright
Copyright © Cambridge University Press 1995

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