Skip to main content Accessibility help
×
Home

The molecular basis of instability of the singedvery weak mutation in Drosophila melanogaster

  • C. A. Ortori (a1), D. Chambers (a1) and J. F. Y. Brookfield (a1)

Summary

The singedvery weak mutation was created by the sequential addition of two P transposable elements to the singed gene. The mutation can be somatically unstable through the action of a dominant maternal effect mutation on the second chromosome. It is also unstable in the germ line in these conditions. Sequencing of the region of the P insertions in the mutation reveals that the two inserted elements have single internal deletions, and the larger of the two is a copy of the KP element. The mutation will generate, at high frequencies, strongly singed and pseudo-wild type products by reversions occurred in the germline. These are the result of the precise excision of the smaller and the larger elements respectively. By PCR amplification of dissected thoraces we show that the somatic instability of the mutation, from a weak to a strong singed phenotype, is also caused by the excision of the smaller of the two elements.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      The molecular basis of instability of the singedvery weak mutation in Drosophila melanogaster
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      The molecular basis of instability of the singedvery weak mutation in Drosophila melanogaster
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      The molecular basis of instability of the singedvery weak mutation in Drosophila melanogaster
      Available formats
      ×

Copyright

Corresponding author

Corresponding author.

References

Hide All
Black, D. M., Jackson, M. S., Kidwell, M. G. & Dover, G. A. (1987). KP-elements repress P-induced dysgenesis in Drosophila melanogaster. EMBO Journal 6, 41254135.
Boussey, I. A., Healy, M. J., Oakeshott, J. G. & Kidwell, M. G. (1988). Molecular analysis of the P-M gonadal dysgenesis cline in Eastern Australian Drosophila melanogaster. Genetics 119, 889902.
Brookfield, J. F. Y. (1991). Models of repression of transposition in P-M hybrid dysgenesis by P cytotype and by zygotically encoded repressor proteins. Genetics 128, 471486.
Brookfield, J. F. Y. & Lewis, A. P. (1989). Somatic reversion of P transposable element insertion mutations at the singed locus of Drosophila melanogaster requiring specific P insertions and a trans-acting factor. Genetical Research 54, 101112.
Brookfield, J. F. Y. & Mitchell, S. F. (1985). P-M hybrid dysgenesis using geographically separate P strains of Drosophila melanogaster. Heredity 55, 163165.
Engels, W. R. (1989). P elements in Drosophila melanogaster. In Mobile DNA (ed. Berg, D. E. and Howe, M. M.), pp. 437484. Washington D.C.: American Society for Microbiology.
Engels, W. R., Johnson-Schlitz, D. M., Eggleston, W. B. & Sved, J. (1990). High-frequency P element loss in Drosophila is homolog dependent. Cell 62, 515525.
Gloor, G. B., Nassif, N. A., Johnson-Schlitz, D. M., Preston, C. R. & Engels, W. R. (1991). Targeted gene replacement in Drosophila via P-element-induced gap repair. Science 253, 11101117.
Heath, E. M. & Simmons, M.J. (1991). Genetic and molecular analysis of repression in the P-M system of hybrid dysgenesis in Drosophila melanogaster. Genetical Research 57, 213226.
Higuet, D., Anxolabéhère, D. & Nouaud, D. (1992). A particular P-element insertion is correlated to the P-induced hybrid dysgenesis repression in Drosophila melanogaster. Genetical Research 60, 1524.
Jackson, M. S., Black, D. M. & Dover, G. A. (1988). Amplification of P elements associated with the repression of hybrid dysgenesis in Drosophila melanogaster. Genetics 120, 10031013.
Jowett, T. (1986). Preparation of nucleic acids. In Drosophila: a practical approach (ed. Roberts, D. B.), pp. 275286. Oxford: IRL Press.
Kitamura, T., Kobayashi, S. & Okada, M. (1993). Developmentally-regulated splicing of the third intron of the P element in somatic tissues of Drosophila embryos. Development, Growth and Differentiation 35, 6773.
Kobayashi, S., Kitamura, T., Sasaki, H. & Okada, M. (1993). Two types of pole cells are present in the Drosophila embryo, one with and one without splicing activity for the third P element intron. Development 117, 885893.
Lewis, A. P. (1987). Ph.D. thesis, University of Leicester.
Misra, S. & Rio, D. C. (1990). Cytotype control of Drosophila P element transposition: the 66 kD protein is a repressor of transposase activity. Cell 62, 269284.
Monastiroti, M., Hatzopoulos, P., Stamatis, N., Yanno-poulos, G. & Louis, C. (1988). Cohabitation of KP and full-length P elements in the genome of MR strains inducing P-M-like hybrid dysgenesis in Drosophila melanogaster. Molecular and General Genetics 215, 9499.
Nitasaka, E., Mukai, T. & Yamazaki, T. (1987). Repressor of P elements in Drosophila melanogaster: cytotype determination by a defective P element with only open reading frames 0 through 2. Proceedings of the National Academy of Science U.S.A. 84, 76057608.
O'Hare, K. & Rubin, G. M. (1983). Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell 34, 2535.
O'Hare, K., Driver, A., McGrath, S. & Johnson-Schlitz, D. M. (1992). Distribution and structure of cloned P elements from the Drosophila melanogaster P strain Π2. Genetical Research 60, 3341.
Paterson, J. & O'Hare, K. (1991). Structure and transcription of the singed locus of Drosophila melanogaster. Genetics 129, 10731084.
Raymond, J. D., Ojala, T. A., White, J. & Simmons, M. J. (1991). Inheritance of P-element regulation in Drosophila melanogaster. Genetical Research 57, 227234.
Rio, D. C. (1990). Molecular mechanisms regulating Drosophila P element transposition. Annual Review of Genetics 24, 543578.
Roiha, H., Rubin, G. M. & O'Hare, K. (1987). P-element insertions and rearrangements at the singed locus of Drosophila melanogaster. Genetics 119, 7583.
Ronsseray, S., Lehmann, M. & Anxolabéhère, D. (1991). The maternally inherited regulation of P elements in Drosophila melanogaster can be elicited by two P copies at cytological site 1A on the X chromosome. Genetics 129, 501512.
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular cloning: a laboratory manual. Cold Spring Harbor Press. Cold Spring Harbor, New York.
Simmons, M. J. & Buchholz, L. M. (1985). Transposase titration in Drosophila melanogaster: a model of cytotype in the P-M system of hybrid dysgenesis. Proceedings of the National Academy of Sciences USA 82, 81198123.
Simmons, M. J., Raymond, J. D., Boedigheimer, M. J. & Zunt, J. R. (1987). The influence of nonautonomous P elements on hybrid dysgenesis in Drosophila melanogaster. Genetics 117, 671685.
Simmons, M. J., Raymond, J. D., Rasmusson, K. E., Miller, L. M., McLarnon, C. F. & Zunt, J. R. (1990). Repression of P element-mediated hybrid dysgenesis in Drosophila melanogaster. Genetics 124, 663676.
Winship, P. R. (1989). An improved method for directly sequencing PCR-amplified material using DMSO. Nucleic Acids Research 17, 1266.

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed