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Isolation and properties of mutants of the FP2 sex factor of Pseudomonas aeruginosa

Published online by Cambridge University Press:  14 April 2009

Vilma A. Stanisich
Vilma A. Stanisich
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria 3168, Australia

Summary

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Two new types of mutants of the FP2 sex factor have been isolated in males of P. aeruginosa strain PAT. Males harbouring FPd mutants are unable to mediate either sex factor or host chromosome transfer, although they retain the exclusion and precipitation characteristics of wild-type males. Males harbouring the FPs mutant apparently have an altered cell surface as indicated by their loss of precipitability, and although their donor properties are similar to those of wild-type males they show a slightly reduced conjugal fertility.

A previously described sex factor mutant FP* (Stanisich & Holloway, 1972) can be transferred to males carrying either the FPs or FPd factors, and the heterozygous strains produced show restoration of wild-type properties, i.e. wild-type precipitation characteristics of the FPs mutants, and transferability of FPd mutants respectively. This suggests that the mutations of the FPs and FPd factors are recessive to the alleles carried by the FP* factor. The ability to produce such heterozygous strains supports the view that at least two copies of the FP2 factor occur in strain PAT males.

Type
Research Article
Information
Genetics Research , Volume 22 , Issue 1 , August 1973 , pp. 13 - 24
Copyright
Copyright © Cambridge University Press 1973

References

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