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Isolation and characterization of deficiencies exposing the rudimentary locus of Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Fardos N. M. Naguib  and
Bruno Jarry
Fardos N. M. Naguib
Affiliation:
Centre National de la Recherche Scientifique, Centre de Biochimie et de Biologie Moléculaire, 31 chemin Joseph Aiguier, 13274 Marseille Cédex 2 (France)
Bruno Jarry
Affiliation:
Centre National de la Recherche Scientifique, Centre de Biochimie et de Biologie Moléculaire, 31 chemin Joseph Aiguier, 13274 Marseille Cédex 2 (France)
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The rudimentary locus of Drosophila melanogaster contains the structural information for the first three enzymes of the de novo pyrimidine pathway. Two new rudimentary (r) mutants have been isolated following mutagenesis with ICR-170. From complementation analysis and cyto-genetical observation both were shown to be deficiencies which expose the r locus. Df(1)r19 is deleted from band 14D1 to 15D1 and Df(1)r1D from band 14B6 to 15A2. These deficiencies were combined with several characterized r alleles each giving a single enzymatic defect in one of the first three enzymes of the pyrimidine pathway. An unusual semilethal trans effect was observed in some but not all the combinations. The effect was not observed with a third smaller deficiency which also exposes the rudimentary locus.

Type
Research Article
Information
Genetics Research , Volume 37 , Issue 2 , April 1981 , pp. 199 - 207
Copyright
Copyright © Cambridge University Press 1981

References

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