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Heterochromatin as a factor affecting X-inactivation in interspecific female vole hybrids (Microtidae, Rodentia)

Published online by Cambridge University Press:  14 April 2009

Suren M. Zakian ,
Tatyana B. Nesterova ,
Olga V. Cheryaukene  and
Michail N. Bochkarev
Suren M. Zakian*
Affiliation:
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk 630090, USSR
Tatyana B. Nesterova
Affiliation:
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk 630090, USSR
Olga V. Cheryaukene
Affiliation:
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk 630090, USSR
Michail N. Bochkarev
Affiliation:
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk 630090, USSR
*
* Corresponding author.

Summary

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Female interspecific vole hybrids were examined for the expression of the G6PD and GALA genes on the X chromosomes. When one of the parents was a species with a heterochromatin block on the X, and the other parent was M. arvalis, without a heterochromatin block on the X, preferential expression of the genes of the M. arvalis X was consistently observed. When both parental species had heterochromatin on the X, the parental forms of G6PD and GALA were in about equal proportions in the hybrid females. The results of the cytological identification of the active and inactive X on the metaphase spreads in the hybrid females are in agreement with the biochemical results. It is suggested that the observed phenomenon may be due to a nonrandom inactivation of the X chromosome containing a heterochromatin block in crosses involving M. arvalis and by a random inactivation in those with both parents having heterochromatin blocks on the X chromosomes. These results support our previous suggestion that heterochromatin has an effect on X inactivation in female interspecific vole hybrids.

Type
Research Article
Information
Genetics Research , Volume 58 , Issue 2 , October 1991 , pp. 105 - 110
Copyright
Copyright © Cambridge University Press 1991

References

Gartler, S. M. & Riggs, A. D. (1983). Mammalian X-chromosome inactivation. Annual Review of Genetics 17, 155–190.CrossRefGoogle ScholarPubMed
Grant, S. G. & Chapman, V. M. (1988). Mechanisms of X-chromosome regulation. Annual Review of Genetics 22, 199–233.CrossRefGoogle ScholarPubMed
Kanda, N. (1973). A new differential technique for staining the heteropycnotic X chromosome in female mice. Experimental Cell Research 80, 463–467.CrossRefGoogle ScholarPubMed
Lyon, M. F. (1961). Gene action in the X-chromosome of the mouse (Mus musculus L.) Nature 190, 372–373.CrossRefGoogle ScholarPubMed
Lyon, M. F. (1974). Mechanisms and evolutionary origins of variable X-chromosome activity in mammals. Proceedings of Royal Society (London) B 187, 243–268.Google ScholarPubMed
Serov, O. L. & Zakijan, S. M. (1977). Allelic expression intergenic fox hybrid (Alopex lagopus × Vulpes vulpes). II. Erythrocyte glucose-6-phosphate dehydrogenase in Arctic and silver foxes: purification and properties. Biochemical Genetics 15, 137–146.CrossRefGoogle Scholar
Takagi, N. & Sasaki, M. (1975). Preferential inactivation of the paternally derived X chromosome in the extra-embryonic membranes of the mouse. Nature 256, 640–642.CrossRefGoogle ScholarPubMed
Zakian, S. M., Kulbakina, N. A., Meyer, M. N., Semenova, L. A., Bochkarev, M. N., Radjabli, S. I. & Serov, O. L. (1987). Non-random inactivation of the X-chromosome in interspecific hybrid voles. Genetical Research 50, 23–27.CrossRefGoogle ScholarPubMed
Zakijan, S. M., Kulbakina, N. A., Serov, O. L., Meyer, M. N. & Zharkikh, A. A. (1984). An estimation of the degree of the genetic divergence of sibling species Microtus arvalis and Microtus subarvalis (Rodentia) based on electrophoretic analysis. Biochemical Genetics 22, 1081–1091.CrossRefGoogle ScholarPubMed