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The effect of temperature on recombination frequency in Neurospora crassa

Published online by Cambridge University Press:  14 April 2009

Caroline A. McNelly-Ingle ,
B. C. Lamb  and
L. C. Frost
Caroline A. McNelly-Ingle
Affiliation:
Genetics Laboratory, Department of Botany, University of Bristol
B. C. Lamb
Affiliation:
Genetics Laboratory, Department of Botany, University of Bristol
L. C. Frost
Affiliation:
Genetics Laboratory, Department of Botany, University of Bristol
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In Neurospora crassa, a change of 2·5°C. in incubation temperature produced highly significant changes in the second division segregation frequency of asco (Linkage Group VI). A minimum value was found at 25°C. consistently increasing towards higher and lower temperatures over the range 15° to 30°C.

Where recombination frequencies in proximal and distal marked regions in Linkage Groups I and VI showed a change, this followed the same pattern as for asco with proximal intervals usually affected; but in one cross a distal region, instead of the proximal, showed a significant change. This behaviour supports the suggestion that heritable factors influencing recombination frequency in specific regions of each chromosome may show differences in temperature sensitivity.

Temperature treatments during protoperithecial development prior to meiosis as well as during meiosis had significant effects on recombination frequencies in several cases. Protoperithecia incubated at one temperature and then transferred to a different temperature after conidiation, gave second division segregation frequencies of asco intermediate in value between those obtained with continuous incubation at either of the two temperatures alone.

In the present work with Neurospora, the similarity in the relationship between temperature and recombination frequency with the reported results in Drosophila is discussed and suggests that the common temperature/recombination frequency curve is indicative of a possible direct effect of temperature on the recombination processes. Present knowledge of these processes is insufficient to permit any detailed explanation of the temperature effects found but the observed negative relationship between recombination frequency and temperature over the range 15° to 22·5°C. is discussed in kinetic terms.

The second division segregation frequency of asco was found to be increased at all temperatures tested when the asci were mounted for scoring in strong sucrose solution compared to mounting in water.

Type
Research Article
Information
Genetics Research , Volume 7 , Issue 2 , April 1966 , pp. 169 - 183
Copyright
Copyright © Cambridge University Press 1966

References

REFERENCES

Barratt, R. W., Newmeyer, D., Perkins, D. D. & Garnjobst, L. (1954). Map construction in Neurospora crassa. Adv. Genet. 6, 1–93.CrossRefGoogle ScholarPubMed
Graubard, M. C. (1934). Temperature effects on interference and crossing over. Genetics, 19, 83–94.CrossRefGoogle ScholarPubMed
Hüttig, W. (1931). Uber den Einfluss der Temperatur auf der Keimung und Geschlechts-verteilung bei Brandpilzen. Z. Bot. 24, 529–577.Google Scholar
Lamb, B. C. (1964). Polarized segregation in Ascomycetes. Heredity, Lond. 19, 344.Google Scholar
Lawrence, M. J. (1958). Genotypic control of crossing over in the first chromosome of Drosophila melanogaster. Nature, Lond. 182, 889–890.CrossRefGoogle ScholarPubMed
Lawrence, M. J. (1963). The control of crossing over in the X-chromosome of Drosophila melanogaster. Heredity, Lond. 18, 27–46.CrossRefGoogle Scholar
McNelly, C. A. (1962). Studies on sexual reproduction and the frequency of recombination over a range of temperatures in Neurospora crassa. Ph.D. Thesis, University of Bristol.Google Scholar
McNelly-Ingle, C. A. & Frost, L. C. (1965). The effect of temperature on the production of perithecia by Neurospora crassa. 'J. gen. Microbiol. 38 (in the press).Google Scholar
Mitchell, H. K. (1957). Crossing over and gene conversion in Neurospora. In Chemical Basis of Heredity (McElroy, W. D. & Glass, B., eds.), pp. 94–113. Baltimore: Johns Hopkins Press.Google Scholar
Olive, L. S. (1956). Genetics of Sordaria fimicola. 1. Ascospore colour mutants. Am. J. Bot. 43, 97–107.CrossRefGoogle Scholar
Plough, H. N. (1917). The effect of temperature on crossing over in Drosophila. J. exp. Zool. 24, 147–209.CrossRefGoogle Scholar
Plough, H. N. (1921). Further studies on the effect of temperature on crossing over. J. exp. Zool. 32, 187–202.CrossRefGoogle Scholar
Pollitzer, O. (1940). Einwirkung von Temperatur und Alter. Z. indukt. Abstamm- u. Vererb-Lehre, 78, 129–147.Google Scholar
Rifaat, O. M. (1959). The effect of temperature on crossing over in Neurospora crassa. Genetica, 30, 312–323.CrossRefGoogle Scholar
Rizet, G. & Engelmann, C. (1949). Contributions à l'étude génétique d'un ascomycète tétrasporé: Podospora anserina. Revue Cytol. Biol. vég. 11, 201–304.Google Scholar
Smith, H. F. (1936). The influence of temperature on crossing over in Drosophila. Nature, Lond. 138, 329–330.CrossRefGoogle Scholar
Stern, C. (1926). An effect of temperature on crossing over in the first chromosome of Drosophila melanogaster. Proc. natn. Acad. Sci. U.S.A. 12, 530–532.Google Scholar
Towe, A. M. & Stadler, D. R. (1964). Effects of temperature on crossing over in Neurospora. Genetics, 49, 577–583.CrossRefGoogle ScholarPubMed
Westergaard, M. & Mitchell, H. K. (1947). A synthetic medium favouring sexual reproduction. Am. J. Bot. 34, 573–577.Google Scholar