Hostname: page-component-848d4c4894-xfwgj Total loading time: 0 Render date: 2024-07-02T02:24:35.287Z Has data issue: false hasContentIssue false
  • English
  • Français

Chromosomal fragment responsible for genetic mosaicism in larval body marking of the silkworm, Bombyx mori

Published online by Cambridge University Press:  14 April 2009

Haruhiko Fujiwara ,
Osamu Ninaki ,
Masahiko Kobayashi ,
Jun Kusuda  and
Hideaki Maekawa
Haruhiko Fujiwara*
Affiliation:
Department of Technology, National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
Osamu Ninaki
Affiliation:
Department of Insect Genetics and Breeding, National Institute of Sericultural and Entomological Science, Kobuchizawa, Yamanashi 409–16, Japan
Masahiko Kobayashi
Affiliation:
Laboratory of Sericultural Sciences, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
Jun Kusuda
Affiliation:
Department of Virology and Rickettsiology, National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
Hideaki Maekawa
Affiliation:
Department of Technology, National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
*
* Corresponding author.

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Several genetic mosaics for larval body marking of the silkworm, Bombyx mori, have been induced by X-ray irradiation. It is hypothesized that the occasional loss of chromosomal fragments carrying the genes for body marking during development may give rise to this type of mosaicism. Using pulsed field gel electrophoresis (PFGE), we find that a DNA molecule of about 2·5 megabases (Mb) is present in one type of mosaic (mottled striped strains pSm788 and pSm872), and not in any other strain. This DNA fragment hybridizes strongly with some chorion genes which are less than 6·9 cM away from the ps locus, and hence it corresponds to a chromosomal fragment containing genes for both striped marking (ps) and the chorion. In the non-mottled ps strain, the phenotype before X-ray irradiation, no band was detected either on a PFGE gel or after hybridization with the chorion probe. These results suggest that the mottled ps strains carry short chromosome fragments which are lost differentially during cell divisions.

Type
Research Article
Information
Genetics Research , Volume 57 , Issue 1 , February 1991 , pp. 11 - 16
Copyright
Copyright © Cambridge University Press 1991

References

Chikushi, H. (1972). Genes and Genetical Stocks of the Silkworm. Keigaku Publishing Co., Tokyo.Google Scholar
Chu, G., Vollrath, D. & Davis, R. W. (1986). Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234, 1582–1585.CrossRefGoogle ScholarPubMed
Doira, H. (1983). Linkage maps of Bombyx mori-status quo in 1983. Sericologia 23, 245–269.Google Scholar
Ebinuma, H., Kobayashi, M., Kobayashi, J., Shimada, T. & Yoshitake, N. (1988). The detection of mosaics and polyploids in a hereditary mosaic strain of the silk moth, Bombyx mori, using egg colour mutations. Genetical Research, 51, 223–229.CrossRefGoogle Scholar
Eickbush, T. H. & Kafatos, F. C. (1982). A walk in the chorion locus of Bombyx mori. Cell 29, 633–643.CrossRefGoogle ScholarPubMed
Friedländer, M. & Wahrman, J. (1970). The spindle as a basal body distributor. Journal of Cell Science, 7, 65–89.CrossRefGoogle ScholarPubMed
Goldsmith, M. R. & Clermont-Rattner, E. (1979). Organization of the chorion genes of Bombyx mori, a multi gene family. II. Partial localization of three gene clusters. Genetics 92, 1173–1185.CrossRefGoogle Scholar
Goldsmith, M. R. & Kafatos, F. C. (1984). Developmentally regulated genes in silkmoths. Annual Review of Genetics, 18, 443–487.CrossRefGoogle ScholarPubMed
Levis, R. W. (1989). Viable deletions of a telomere from a Drosophila chromosome. Cell 58, 791–801.CrossRefGoogle ScholarPubMed
Mitsialis, S. A. & Kafatos, F. C. (1985). Regulatory elements controlling chorion gene expression are conserved between flies and moths. Nature 317, 453–456.CrossRefGoogle ScholarPubMed
Murakami, A. & Imai, H. T. (1974). Cytological evidence for holocentric chromosomes of the silkworm, Bombyx mori and B. mandarina, (Bombycidae, Lepidoptera). Chromosoma (Berl.) 47, 167–178.CrossRefGoogle Scholar
Roberts, P. A. (1975). In support of the telomere concept. Genetics 80, 135–142.CrossRefGoogle ScholarPubMed
Schwartz, D. C. & Cantor, C. R. (1984). Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37, 67–75.CrossRefGoogle ScholarPubMed
Smith, C. L., Matsumoto, T., Niwa, O., Klco, S., Fan, J.-B., Yanagida, M. & Cantor, C. R. (1987). An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis. Nucleic Acids Research, 15, 4481–4489.CrossRefGoogle ScholarPubMed
Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology, 98, 503–517.CrossRefGoogle ScholarPubMed
Spoerel, N. A., Nguyen, H. T., Eickbush, T. H. & Kafatos, F. C. (1989). Gene evolution and regulation in the chorion complex of Bombyx mori. Hybridization and sequence analysis of multiple developmentally middle A/B chorion gene pairs. Journal of Molecular Biology, 209, 1–19.CrossRefGoogle ScholarPubMed
Takasaki, T. & Tazima, Y. (1944). Movement of chromosomes in the trisomic and monosomic silkworm. Japanese Journal of Genetics, 20, 75–76. (Japanese).Google Scholar
Tanaka, Y. (1935). ‘Mottled striped’, a mutable due to the chromosome translocation. Science Bulletin of Faculty of Agriculture of Kyushu University, 6, 404–413. (Japanese).Google Scholar
Tazima, Y. (1964). Mosaicism. In The Genetics of the Silkworm. (Logos Press, London), pp. 146–164.Google Scholar
Tazima, Y. (1978). The Silkworm; an important laboratory tool, Kodansha LTD., Tokyo.Google Scholar
Virk, D. S. (1959). Genetical studies on the effects of X-ray in the silkworm, Bombyx mori L. I. Differential frequency of chromosomal fragmentation by X-ray treatment in F1 hybrids of different female parents. Japanese Journal of Genetics, 34, 285–292.Google Scholar
Virk, D. S. (1960). Genetical studies on the effects of X-ray in the silkworm, Bombyx mori L. II. Relationship between growth stage and X-ray-induced chromosomal fragmentation. Japanese Journal of Genetics, 35, 179–186.Google Scholar