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Caenorhabditis elegans as a platform for molecular quantitative genetics and the systems biology of natural variation

  • BRYN E. GAERTNER (a1) and PATRICK C. PHILLIPS (a1)

Summary

Over the past 30 years, the characteristics that have made the nematode Caenorhabditis elegans one of the premier animal model systems have also allowed it to emerge as a powerful model system for determining the genetic basis of quantitative traits, particularly for the identification of naturally segregating and/or lab-adapted alleles with large phenotypic effects. To better understand the genetic underpinnings of natural variation in other complex phenotypes, C. elegans is uniquely poised in the emerging field of quantitative systems biology because of the extensive knowledge of cellular and neural bases to such traits. However, perturbations in standing genetic variation and patterns of linkage disequilibrium among loci are likely to limit our ability to tie understanding of molecular function to a broader evolutionary context. Coupling the experimental strengths of the C. elegans system with the ecological advantages of closely related nematodes should provide a powerful means of understanding both the molecular and evolutionary genetics of quantitative traits.

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*Corresponding author: e-mail: pphil@uoregon.edu

References

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Ajie, B. C., Estes, S., Lynch, M. & Phillips, P. C. (2005). Behavioral degradation under mutation accumulation in Caenorhabditis elegans. Genetics 170, 655660.
Anderson, J. L., Albergotti, L., Proulx, S., Peden, C., Huey, R. B. & Phillips, P. C. (2007). Thermal preference of Caenorhabditis elegans: a null model and empirical tests. Journal of Experimental Biology 210, 31073116.
Anderson, J. L., Morran, L. T. & Phillips, P. C. (2010). Outcrossing and the maintenance of males within C. elegans populations. Journal of Heredity 101, S62S74.
Ayyadevara, A., Thaden, J. J. & Shmookler-Reis, R. J. (2000 a). Anchor polymerase chain reaction display: a high-throughput method to resolve, score and isolate dimorphic genetic markers based on interspersed repetitive DNA elements. Analytical Biochemistry 284, 1928.
Ayyadevara, S., Ayyadevara, R., Hou, S., Thaden, J. J. & Shmookler-Reis, R. J. (2000 b). Genetic mapping of quantitative trait loci governing longevity of Caenorhabditis elegans in recombinant-inbred progeny of a Bergerac-BO×RC301 interstrain cross. Genetics 157, 655666.
Ayyadevara, S., Ayyadevara, R., Vertino, A., Galecki, A., Thaden, J. J. & Shmookler-Reis, R. J. (2003). Genetic loci modulating fitness and life span in Caenorhabditis elegans: categorical trait interval mapping in CL2a×Bergerac-BO recombinant-inbred worms. Genetics 163, 557570.
Azevedo, R. B. R., Keightley, P. D., Lauren-Maatta, C., Vassilieva, L. L., Lynch, M. & Leroi, A. M. (2002). Spontaneous mutational variation for body size in Caenorhabditis elegans. Genetics 162, 755765.
Baer, C. F. (2008). Quantifying the de-canalizing effects of spontaneous mutations in Rhabditid nematodes. American Naturalist 172, 272281.
Baer, C. F. & Denver, D. R. (2010). Spontaneous mutations decrease sensitivity of gene expression to random environmental variation in Caenorhabditis elegans. PLoS ONE 5, e8750.
Baer, C. F., Phillips, N., Ostrow, D., Avalos, A., Blanton, D., Boggs, A., Keller, T., Levy, L. & Mezerhand, E. (2006). Cumulative effects of spontaneous mutations for fitness in Caenorhabditis: role of genotype, environment and stress. Genetics 174, 13871395.
Baer, C. F., Shaw, F., Steding, C., Baumgaartner, M., Hawkins, A., Houppert, A., Mason, N., Reed, M., Simonelic, K., Woodard, W. & Lynch, M. (2005). Comparative evolutionary genetics of spontaneous mutations affecting fitness in rhabditid nematodes. Proceedings of the National Academy of Sciences of the USA 102, 57855790.
Baird, N. A., Etter, P. D., Atwood, T. S., Currey, M. C., Shiver, A. L., Lewis, Z. A., Selker, E. U., Cresko, W. A. & Johnson, E. A. (2008). Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS ONE 3, e3376.
Baird, S. E. (1999). Natural and experimental association of Caenorhabditis remanei with Trachelipus rathkii and other terrestrial isopods. Nematology 1, 471475.
Baird, S. E., Davidson, C. R. & Bohrer, J. C. (2005). The genetics of ray pattern variation in Caenorhabditis briggsae. BMC Evolutionary Biology 5, 3.
Braendle, C., Baer, C. & Felix, M. (2010). Bias and evolution of the mutationally accessible phenotypic space in a developmental system. PLoS Genetics 6, e1000877.
Braendle, C. & Felix, M. (2008). Plasticity and errors of a robust developmental system in different environments. Developmental Cell 15, 714724.
Braendle, C. & Felix, M. (2009). The other side of phenotypic plasticity: a developmental system that generates an invariant phenotype despite environmental variation. Journal of Bioscience 34, 543551.
Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 7194.
Bost, B., de Vienne, D., Hospital, F., Moreau, L. & Dillman, C. (2001). Genetic and nongenetic bases for the L-shaped distribution of quantitative trait loci effects. Genetics 157, 17731787.
Brooke, A. & Johnson, T. E. (1991). Genetic specification of life span and self-fertility in recombinant-inbred strains of Caenorhabditis elegans. Heredity 67, 1928.
C. elegans Sequencing Consortium. (1998). Genome sequence of the nematode C. elegans: a platform for investigating biology. Science 282, 20122018.
Chalasani, S. H., Chronia, N., Tsunozaki, M., Gray, J. M., Ramot, D., Goodman, M. B. & Bargmann, C. I. (2007). Dissecting a circuit for olfactory behavior in Caenorhabditis elegans. Nature 450, 6370.
Chan, Y. F., Marks, M. E., Jones, F. C., Villarreal, G., Shapiro, M. D., Brady, S. D., Soutwick, A. M., Absher, D. M., Grimwood, J., Schmutz, J., Myers, R. M., Petrov, D., Jonsson, B., Schluter, D., Bell, M. A. & Kingsley, D. M. (2010). Adaptive evolution of pelvic reduction in sticklebacks by recurrent deletion of a Pitx-1 enhancer. Science 327, 302305.
Chandler, C. H. (2010). Cryptic intraspecific variation in sex determination in Caenorhabditis elegans revealed by mutations. Heredity 105, 473482.
Charlesworth, B., Morgan, M. T. & Charlesworth, D. (1993). The effect of deleterious mutations on neutral molecular variation. Genetics 134, 12891303.
Chasnov, J. R., So, W. K., Chan, C. M. & Chow, K. L. (2007). The species, sex & stage specificity of a Caenorhabditis sex pheromone. Proceeedings of the National Academy of Sciences of the USA 104, 67306735.
Chen, Y., Zhu, J., Lum, P. Y., Yang, Z., Pinto, S., MacNeil, D. J., Zhang, C., Lamb, J., Edwards, S., Sieberts, S. K., Leonardson, A., Castellini, L. W., Wang, S., Champy, M. F., Zhang, B., Emilsson, V., Doss, S., Ghazalpour, A., Horvath, S., Drake, T., Lusis, A. J. & Schadt, E. E. (2008). Variations in DNA elucidate molecular networks that cause disease. Nature 452, 429435.
Cutter, A. D. (2006). Nucleotide polymorphism and linkage disequilibrium in wild populations of the partial selfer Caenorhabditis elegans. Genetics 172, 171184.
Cutter, A. D., Baird, S. E. & Charlesworth, D. (2006). High nucleotide polymorphism and rapid decay of linkage disequilibrium in wild populations of Caenorhabditis remanei. Genetics 174, 901913.
Cutter, A. D. & Payseur, B. A. (2003). Selection at linked sites in the partial selfer Caenorhabditis elegans. Molecular Biology and Evolution 20, 665673.
Darvasi, A. & Soller, M. (1995). Advanced intercross lines, an experimental population for fine genetic mapping. Genetics 141, 11991207.
Davies, A. G., Bettinger, J. C., Thiele, T. R., Judy, M. E. & McIntire, S. L. (2004). Natural variation in the npr-1 gene modifies ethanol responses of wild strains of C. elegans. Neuron 42, 731743.
de Bono, M. & Bargmann, C. I. (1998). Natural variation in a neuropeptide Y receptor homolog modifies social behavior and food response in C. elegans. Cell 94, 679689.
Delattre, M. & Felix, M. A. (2001). Polymorphism and evolution of vulval precursor cell lineages within two nematode genera, Caenorhabditis and Oscheius. Current Biology 11, 631643.
Denver, D. R., Dolan, P. C., Wilhelm, L. J., Sung, W., Lucas-Lledo, J. I., Howe, D. K., Lewis, S. C., Okamoto, K., Thomas, W. K., Lynch, M. & Baer, C. F. (2009). A genome-wide view of Caenorhabditis elegans base-substitution mutation processes. Proceedings of the National Academy of Sciences of the USA 106, 1631016314.
Denver, D. R., Howe, D. K., Wilhelm, L. J., Palmer, C. A., Anderson, J. L., Stein, K. C., Phillips, P. C. & Estes, S. (2010). Selective sweeps and parallel mutation in the adaptive recovery from deleterious mutation in Caenorhabditis elegans. Genome Research, in press.
Denver, D. R., Morris, K., Kewalramani, A., Harris, K. E., Chow, A., Estes, S., Lynch, M. & Thomas, W. K. (2004). Abundance, distribution and mutation rates of homopolymeric nucleotide runs in the genome of Caenorhabditis elegans. Journal of Molecular Evolution 58, 587595.
Denver, D. R., Morris, K., Lynch, M., Vassilieva, L. L. & Thomas, W. K. (2000). High direct estimate of the mutation rate in the mitochondrial genome of Caenorhabditis elegans. Science 289, 23422344.
Denver, D. R., Morris, K. & Thomas, W. K. (2003). Phylogenetics in Caenorhabditis elegans: an analysis of divergence and outcrossing. Molecular Biology and Evolution 20, 393400.
Dolgin, E. S., Felix, M. A. & Cutter, A. D. (2008). Hakuna Nematoda: genetic and phenotypic diversity in African isolates of Caenorhabditis elegans and C. briggsae. Heredity 100, 304315.
Doroszuk, A., Snoek, L. B., Fradin, E., Riksen, J. & Kammenga, J. (2009). A genome-wide library of CB4856/N2 introgression lines of Caenorhabditis elegans. Nucleic Acids Research 37, e110.
Ebert, R. H., Cherkasova, V. A., Dennis, R. A., Wu, J. H., Ruggles, S., Perrin, T. E. & Shmookler-Reis, R. J. (1993). Longevity-determining genes in Caenorhabditis elegans: chromosomal mapping of multiple noninteractive loci. Genetics 135, 10031010.
Ebert, R. H., Shammas, M. A., Sohal, B. H., Sohal, R. S., Egilmez, N. K., Ruggles, S. & Shmookler-Reis, R. J. (1996). Defining genes that govern longevity in Caenorhabditis elegans. Developmental Genetics 18, 131143.
Egilmez, N. K., Ebert, R. H. & Shmookler-Reis, R. J. (1995). Strain evolution in Caenorhabditis elegans: transposable elements as markers of interstrain evolutionary history. Journal of Molecular Evolution 40, 372381.
Emmons, S. W., Yesner, L., Ruan, K. & Katzenberg, D. (1983). Evidence for a transposon in Caenorhabditis elegans. Cell 32, 5565.
Estes, S., Ajie, B. C., Lynch, M. & Phillips, P. C. (2005). Spontaneous mutational correlations for life-history, morphological and behavioral characters in Caenorhabditis elegans. Genetics 170, 645653.
Estes, S. & Lynch, M. (2003). Rapid fitness recovery in mutationally degraded lines of Caenorhabditis elegans. Evolution 57, 10221030.
Estes, S. & Phillips, P. C. (2006). Variation in pleiotropy and the mutational underpinnings of the G-matrix. Evolution 60, 26552660.
Estes, S., Phillips, P. C., Denver, D. R., Thomas, W. K. & Lynch, M. (2004). Mutation accumulation in populations of varying size: implications for the distribution of mutational effects on fitness. Genetics 166, 12691279.
Fay, D. (2006). Genetic mapping and manipulation: Chapter 5-SNPs: Three-point mapping., WormBook, ed. The C. elegans Research Community, WormBook. doi:10·1895/wormbook.1.94.1. Available at http://www.wormbook.org.
Friedman, D. B. & Johnson, T. E. (1988). A mutation in the age-1 gene in Caenorhabditis elegans lengthens life and reduces hermaphrodite fertility. Genetics 118, 7586.
Graustein, A., Gaspar, J. M., Walters, J. R. & Palopoli, M. F. (2002). Levels of DNA polymorphism vary with mating system in the nematode genus Caenorhabditis. Genetics 161, 99–107.
Gutteling, E. W., Doroscuk, A., Riksen, J. A. G., Prokop, Z., Reszka, J. & Kammenga, J. E. (2007 a). Environmental influence on the genetic correlations between life-history traits in Caenorhabditis elegans. Heredity 98, 206213.
Gutteling, E. W., Riksen, J. A. G., Bakker, J. & Kammenga, J. E. (2007 b). Mapping phenotypic plasticity and genotype-environment interactions affecting life-history traits in Caenorhabditis elegans. Heredity 98, 2837.
Hartl, D. L. & Clark, A. G. (2007). Principles of Population Genetics, 4th edn. Sunderland, MA: Sinauer Associates, Inc.
Harvey, S. C. (2009). Non-dauer larval dispersal in Caenorhabditis elegans. Journal of Experimental Zoology B: Molecular and Developmental Evolution 312, 224230.
Harvey, S. C., Barker, G. L. A., Shorto, A. & Viney, M. E. (2009). Natural variation in gene expression in the early development of dauer larvae of Caenorhabditis elegans. BMC Genomics 10, 325.
Harvey, S. C., Shorto, A. & Viney, M. E. (2008). Quantitative genetic analysis of life-history traits of Caenorhabditis elegans in stressful environments. BMC Evolutionary Biology 8, 15.
Harvey, S. C. & Viney, M. E. (2007). Thermal variation reveals natural variation between isolates of Caenorhabditis elegans. Journal of Experimental Zoology B: Molecular and Developmental Evolution 308, 409416.
Hodgkin, J. & Doniach, T. (1997). Natural variation and copulatory plug formation in Caenorhabditis elegans. Genetics 146, 149164.
Hu, P. J. (2007). Dauer. WormBook, ed. The C. elegans Research Community, WormBook. doi:10.1895/wormbook.1.144.1. Available at http://www.wormbook.org.
Huang, C., Xiong, C. & Kornfeld, K. (2004). Measurements of age-related changes of physiological processes that predict lifespan of Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the USA 101, 80848089.
Johnson, T. E. (1987). Aging can be genetically dissected into component processes using long-lived lines of Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the USA 84, 27772781.
Johnson, T. E. (2007). Caenorhabditis elegans 2007: the premier model for the study of aging. Experimental Gerontology 43, 10–4.
Johnson, T. E. & Hutchinson, E. W. (1993). Absence of strong heterosis for life span and other life history traits in Caenorhabditis elegans. Genetics 134, 465474.
Johnson, T. E. & Wood, W. B. (1982). Genetic analysis of life-span in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the USA 79, 66036607.
Jorgensen, E. M. & Mango, S. E. (2002). The art and design of genetic screens: Caenorhabditis elegans. Nature Reviews Genetics 3, 356369.
Jovelin, R., Ajie, B. C. & Phillips, P. C. (2003). Molecular evolution and quantitative variation for chemosensory behaviour in the nematode genus Caenorhabditis. Molecular Ecology 12, 13251337.
Jovelin, R., Dunham, J. P., Sung, F. S. & Phillips, P. C. (2009). High nucleotide divergence in developmental regulatory genes contrasts with the structural elements of olfactory pathways in Caenorhabditis. Genetics 181, 13871397.
Joyner-Matos, J., Upadhyay, A., Salomon, M. P., Grigaltchik, V. & Baer, C. F. (2009). Genetic (co)variation for life span in rhabditid nematodes: role of mutation, selection and history. Journal of Gerontology 64A, 11341145.
Kammenga, J. E., Doroszuk, A., Riksen, J. A. G., Hazendonk, E., Spiridon, L., Petrescu, A. J., Tijsterman, M., Plasterk, R. H. A. & Bakker, J. (2007). A Caenorhabditis elegans wild type defies the temperature-size rule owing to a single nucleotide polymorphism in tra-3. PLoS Genetics 3, 03580366.
Kammenga, J. E., Phillips, P. C., de Bono, M. & Doroscuk, A. (2008). Beyond induced mutants: using worms to study natural variation in genetic pathways. Trends in Genetics 24, 178185.
Keightley, P. D. & Caballero, A. (1997). Genomic mutation rates for lifetime reproductive output and lifespan in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the USA 94, 38233827.
Keightley, P. D. & Eyre-Walker, A. (2000). Deleterious mutations and the evolution of sex. Science 290, 331333.
Kiontke, K., Gavin, N. P., Raynes, Y., Roehrig, C., Piano, F. & Fitch, D. H. A. (2004). Caenorhabditis phylogeny predicts convergence of hermaphroditism and extensive intron loss. Proceedings of the National Academy of Sciences of the USA 101, 90039008.
Knight, C. G., Azvedo, R. B. R. & Leroy, A. M. (2001). Testing life-history pleiotropy in Caenorhabditis elegans. Evolution 55, 17951804.
Koch, R., van Luenen, H. G., van der Horst, M., Thijssen, K. L. & Plasterk, R. H. (2000). Single nucleotide polymorphisms in wild isolates of Caenorhabditis elegans. Genome Research 10, 16901696.
Lande, R. (1982). A quantitative genetic theory of life history evolution. Ecology 63, 607615.
Lander, E. S. & Botstein, D. (1989). Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121, 185189.
Li, Y., Alvarez, O. A., Gutteling, E. W., Tijsterman, M., Fu, J., Riksen, J. A. G., Hazendonk, E., Prins, P., Plasterk, R. H. A., Jansen, R. C., Breitling, R. & Kammenga, J. E. (2006). Mapping determinant of gene expression plasticity by genetical genomics in C. elegans. PLoS Genetics 2, 21552161.
Li, Y., Breitling, R., Snoek, L. B., van der Velde, K. J., Swertz, M. A., Riksen, J., Jansen, R. C. & Kammenga, J. E. (2010). Global genetic robustness of the alternative splicing machinery in Caenorhabditis elegans. Genetics 186, 405410.
Lockery, S. R., Lawton, K. J., Doll, J. C., Faumont, S., Coulthard, S. M., Thiele, T. R., Chronis, N., McCormick, K. E., Goodman, M. B. & Pruitt, B. L. (2008). Artificial dirt: microfluidic substrates for nematode neurobiology and behavior. Journal of Neurophysiology 99, 31363143.
Macosko, E. Z., Pokala, N., Feinberg, E. H., Chalasani, S. H., Butcher, R. A., Clardy, J. & Bagmann, C. I. (2009). A hub-and-spoke circuit drives pheromone attraction and social behaviour in C. elegans. Nature 458, 11711175.
McGrath, P. T., Rockman, M. V., Zimmer, M., Jang, H., Macosko, E. Z., Kruglyak, L. & Bargmann, C. I. (2009). Quantitative mapping of a digenic behavioral trait implicates globin variation in C. elegans sensory behaviors. Neuron 61, 692699.
Meneely, P. M., Farago, A. F. & Kauffman, T. M. (2002). Crossover distribution and high interference for both the X chromosome and an autosome during oogenesis and spermatogenesis in Caenorhabditis elegans. Genetics 162, 11691177.
Milloz, J., Duveau, F., Nuez, I. & Felix, M. A. (2008). Intraspecific evolution of the intercellular signaling network underlying a robust developmental system. Genes and Development 22, 30643075.
Moerman, D. G. & Waterston, R. H. (1984). Spontaneous unstable unc-122 IV mutation in C. elegans var. Bergerac. Genetics 108, 859877.
Mori, I., Moerman, D. G. & Waterson, R. H. (1988). Analysis of a mutator activity necessary for germline transposition and excision of Tc1 transposable elements in Caenorhabditis elegans. Genetics 120, 397407.
Mori, I., Moerman, D. G. & Waterston, R. H. (1990). Interstrain crosses enhance excision of Tc1 transposable elements in Caenorhabditis elegans. Molecular and General Genetics 220, 251255.
Morran, L. T., Parmenter, M. D. & Phillips, P. C. (2009). Mutation load and rapid adaptation favour outcrossing over self-fertilization. Nature 462, 350352.
Ostrow, D. G., Phillips, N., Avalos, A., Blanton, D., Boggs, A., Keller, T., Levy, L., Rosenbloom, J. & Baer, C. F. (2007). Mutational bias for body size in Rhabditid nematodes. Genetics 176, 16531661.
Palopoli, M. F., Rockman, M. V., TinMaung, A., Ramsay, C., Curwen, W., Aduna, A., Laurita, J. & Kruglyak, L. (2008). Molecular basis of the copulatory plug polymorphism in Caenorhabditis elegans. Nature 454, 10191023.
Persson, A., Gross, E., Laurent, P., Busch, K. E., Bretes, H. & de Bono, M. (2009). Natural variation in a neural globin tunes oxygen sensing in wild Caenorhabditis elegans. Nature 458, 10301033.
Phillips, N., Salomon, M., Custer, A., Ostrow, D. & Baer, C. F. (2009). Spontaneous mutational and standing genetic (Co)variation at dinucleotide microsatellites in Caenorhabditis briggsae and C. elegans. Molecular Biology and Evolution 26, 659669.
Phillips, P. C. (2006). One perfect worm. Trends in Genetics 22, 405407.
Pocock, R. & Hobart, O. (2010). Hypoxia activates a latent circuit for processing gustatory information in C. elegans. Nature Neuroscience 13, 610614.
Prasad, A., Croydon-Sugarman, M. J. F., Murray, R. L. & Cutter, A. D. (2010). Temperature-dependent fecundity associates with latitude in Caenorhabditis briggsae. Evolution, in press.
Rebeiz, M., Pool, J. E., Kassner, V. A., Aquadro, C. F. & Carroll, S. B. (2009). Stepwise modification of a modular enhancer underlies adaptation in a Drosophila population. Science 326, 16631667.
Reddy, K. C., Anderson, E. C., Kruglyak, L. & Kim, D. H. (2009). A polymorphism in npr-1 is a behavioral determinant of pathogen susceptibility in C. elegans. Science 323, 382384.
Rockman, M. V. (2008). Reverse engineering the genotype-phenotype map with natural genetic variation. Nature 456, 738744.
Rockman, M. V. & Kruglyak, L. (2006). Genetics of global gene expression. Nature 7, 862872.
Rockman, M. V. & Kruglyak, L. (2008). Breeding designs for recombinant inbred advanced intercross lines. Genetics 179, 10691078.
Rockman, M. V. & Kruglyak, L. (2009). Recombinational landscape and population genomics of Caenorhabditis elegans. PLoS Genetics 5, e1000419.
Rockman, M. V., Skrovanek, S. S. & Kruglak, L. (2010). Selection at linked sites shapes heritable phenotype variation in C. elegans. Science 15, 372376.
Rohde, C. B., Zeng, F., Gonzalez-Rubio, R., Angel, M. & Yanik, M. F. (2007). Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution. Proceedings of the National Academy of Sciences of the USA 104, 1389113895.
Rual, J. F., Ceron, J., Koreth, J., Hao, T., Nicot, A. S., Hirozane-Kishikawa, T., Vandenhaute, J., Orkin, S. H., Hill, D. E., van den Heuvel, S. & Vidal, M. (2004). Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Research 14, 21622168.
Salomon, M. P., Ostrow, D., Phillips, N., Blanton, D., Bour, W., Kepper, T., Levy, L., Sylvestre, T., Upadhyay, A. & Baer, C. F. (2009). Comparing mutational and standing genetic variability for fitness and size in Caenorhabditis briggsae and C. elegans. Genetics 183, 18.
Schadt, E. E., Monks, S. A., Drake, T. A., Lusis, A. J., Che, N., Colinayo, V., Ruff, T. G., Milligan, S. B., Lamb, J. R., Cavet, G., Linsley, P. S., Mao, M., Stoghton, R. B. & Friend, S. H. (2003). Genetics of gene expression surveyed in maize, mouse and man. Nature 422, 297302.
Shmookler-Reis, R. J., Kang, P. & Ayyadevara, S. (2006). Quantitative trait loci define genes and pathways underlying genetic variation in longevity. Experimental Gerontology 41, 10461054.
Shook, D. R., Brooks, A. & Johnson, T. E. (1996). Mapping quantitative trait loci affecting life history traits in the nematode Caenorhabditis elegans. Genetics 142, 801817.
Shook, D. R. & Johnson, T. E. (1999). Quantitative trait loci affecting survival and fertility-related traits in Caenorhbaditis elegans show genotype–environment interaction, pleiotropy and epistasis. Genetics 153, 12331243.
Siedel, H. S., Rockman, M. V. & Kruglyak, L. (2008). Widespread genetic incompatibility in C. elegans maintained by balancing selection. Science 319, 589594.
Steiner, C. C., Weber, J. N. & Hoekstra, H. E. (2007). Adaptive variation in beach mice produced by two interacting pigment genes. PLoS Biology 5, e219.
Teotónio, H., Manoel, D. & Phillips, P. C. (2006). Genetic variation for outcrossing among Caenorhabditis elegans isolates. Evolution 60, 13001305.
Thomas, J. H. (1998). Social life and the single nucleotide: foraging behavior in C. elegans. Cell 94, 549550.
Toth, A. L. & Robinson, G. E. (2007). Evo-devo and the evolution of social behavior. Trends in Genetics 23, 334341.
van Swinderen, B., Galifianakis, A. & Crowder, C. M. (1998 a). A quantitative genetic approach toward volatile anesthetic mechanisms in C. elegans. Toxicology Letters 100–101, 309–307.
van Swinderen, B., Metz, L. B., Shebester, L. D., Mendel, J. E., Sternberg, P. W. & Crowder, C. M. (2001). Goα regulates volatile anesthetic action in Caenorhabditis elegans. Genetics 158, 643655.
van Swinderen, B., Shook, D. R., Ebert, R. H., Cherkasova, V. A., Johnson, T. E., Shmookler-Reis, R. J. & Crowder, C. M. (1997). Quantitative trait loci controlling halothane sensitivity in Caenorhabditis elegans. MNAS Pharmacology 94, 82328237.
van Swinderen, B. B. A., Galifianakis, A. & Crowder, C. M. (1998 b). Common genetic determinants of halothane and isoflurane potencies in Caenorhabditis elegans. Anesthesiology 89, 15091517.
Vassilieva, L. L., Hook, A. M. & Lynch, M. (2007). The fitness effects of spontaneous mutations in Caenorhabditis elegans. Evolution 5, 12341246.
Vassilieva, L. L. & Lynch, M. (1999). The rate of spontaneous mutation for life-history traits in Caenorhabditis elegans. Genetics 151, 119129.
Vieira, C., Pasyukova, E. G., Zeng, Z. B., Hackett, J. B., Lyman, F. R. & Mackay, T. F. C. (2000). Genotype-environment interaction for quantitative trait loci affecting life span in Drosophila melanogaster. Genetics 154, 213227.
Viney, M. E., Gardner, M. P. & Jackson, J. A. (2003). Variation in Caenorhabditis elegans dauer larva formation. Development, Growth and Differentiation 45, 389396.
Viñuela, A., Snoek, L. B., Riksen, J. A. G. & Kammenga, J. E. (2010). Genome-wide gene expression regulation as a function of genotype and age in C. elegans. Genome Research 20, 929937.
Wang, G.-X., Ren, S., Ren, Y., Ai, H. & Cutter, A. D. (2010). Extremely high molecular diversity within East Asian nematode Caenorhabditis sp. 5. Molecular Ecology 19(22), 50225029.
Wang, Z. W., Saifee, O., Nonet, M. L. & Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C.elegans neuromuscular junction. Neuron 32, 867881.
Wicks, S. R., Yeh, R. T., Gish, W. R., Waterson, R. H. & Plasterk, R. H. A. (2001). Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map. Nature Genetics 28, 160164.
Williams, B. D., Schrank, B., Huynh, C., Shownkeen, R. & Waterston, R. H. (1992). A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics 131, 609624.

Caenorhabditis elegans as a platform for molecular quantitative genetics and the systems biology of natural variation

  • BRYN E. GAERTNER (a1) and PATRICK C. PHILLIPS (a1)

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