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An easy way of obtaining Aspergillus nidulans haploids in the parasexual cycle using N-glycosyl polifungin

Published online by Cambridge University Press:  14 April 2009

Jerzy Bal ,
Ewa Bartnik ,
Bożenna Goryluk  and
Norman J. Pieniążek
Jerzy Bal
Affiliation:
Department of Genetics, University of Warsaw, Warsaw, Al. Ujazdowskie 4, 00478, Poland
Ewa Bartnik
Affiliation:
Department of Genetics, University of Warsaw, Warsaw, Al. Ujazdowskie 4, 00478, Poland
Bożenna Goryluk
Affiliation:
Department of Genetics, University of Warsaw, Warsaw, Al. Ujazdowskie 4, 00478, Poland
Norman J. Pieniążek
Affiliation:
Department of Genetics, University of Warsaw, Warsaw, Al. Ujazdowskie 4, 00478, Poland
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Summary

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A considerable improvement in the selection of haploids in Aspergillus nidulans is described. Haploidization is used to assign genes to linkage groups (chromosomes) in fungi. The differential effect of a polyene antibiotic, N-glycosyl-polifungin, in respect to haploids and diploids, was utilized. In minimal medium only diploids (constructed from a ‘Master Strain’ and the investigated strain), which are fully heterozygous and thus have no growth requirements, germinate and are killed by the antibiotic. However, auxotrophic haploids, either induced or spontaneously formed, survive. These can then be used for linkage studies.

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 3 , June 1975 , pp. 249 - 252
Copyright
Copyright © Cambridge University Press 1975

References

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