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Allelism of methionine-sensitive mutants of Schizosaccharomyces pombe to loci involved in adenine biosynthesis

Published online by Cambridge University Press:  14 April 2009

Andre Strauss
Andre Strauss
Affiliation:
Institute of General Microbiology, University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland
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Summary

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Methionine-sensitive mutants of Schizosaccharomyces pombe were found to map at 16 loci, min1–min16, seven of which are allelic to known ade loci involved in adenine biosynthesis. The mutant min1–106 was shown to be located within the ade6 locus by intragenic recombination analysis. Methionine sensitivity of these mutants is reversible by adenine supplementation and their pigmentation is similar to that of the corresponding ade mutants. We conclude, therefore, that these min mutants are incompletely blocked in adenine biosynthesis. These observations further indicate that adenine biosynthesis in S. pombe is inhibited by methionine or a metabolite derived from it. For some other min mutants showing reversibility of methionine sensitivity by leucine or proline, no allelism to known leu or pro loci was detected.

Type
Research Article
Information
Genetics Research , Volume 33 , Issue 3 , June 1979 , pp. 261 - 268
Copyright
Copyright © Cambridge University Press 1979

References

REFERENCES

Clarke, C. H. (1965). Methionine as an antimutagen in Schizoaaccharomyces pombe. Journal of General Microbiology 39, 21–31.CrossRefGoogle ScholarPubMed
Cutts, N. S. & Rainbow, C. (1950). Methionine and the formation of pigment by yeasts. Nature 166((4235), 1117.CrossRefGoogle ScholarPubMed
Dorfman, B. (1969). The isolation of adenylosuccinate synthetase mutants in yeast by selection for constitutive behavior in pigmented strains. Genetics 61, 377–389.CrossRefGoogle ScholarPubMed
Fluri, R., Coddington, A. & Flury, U. (1976). The product of the ade1 gene in Schizosaccharomyces pombe: A bifunctional enzyme catalysing two distinct steps in purine biosynthesis. Molecular & General Genetics 147, 271–282.CrossRefGoogle ScholarPubMed
Gutz, H. (1963). Untersuchungen zur Feinstruktur der Gene ad 7, und ad 6 von Schizosaccharomyces pombe Lind. Habilitation thesis, Technische Universität Berlin.Google Scholar
Gutz, H., Heslot, H., Leupold, U. & Loprieno, N. (1974). Schizosaccharomyces pombe. Handbook of Genetics, 1, 395–445.Google Scholar
Kohli, J., Hottinger, H., Munz, P., Strauss, A. & Thuriaux, P. (1977). Genetic mapping in Schizosaccharomyces pombe by mitotic and meiotic analysis and induced haploidization. Genetics 87, 471–489.CrossRefGoogle ScholarPubMed
Leupold, U. (1955). Versuche zur genetischen Klaesifizierung adeninabhängiger Mutanten von Schizosaccharomyces pombe. Archiv der Julius Klaus-Stiftung für Vererbungsforschung, Sozialanthropologie und Rassenhygiene 30, 506–516.Google Scholar
Leupold, U. (1970). Genetioal methods for Schizosaccharomyces pombe. Methods in Cell Physiology 4, 169–177.CrossRefGoogle Scholar
Meuris, P., Lacroute, F. & Slonimski, P. O. (1967). Etude systématique de mutants inhibés par leurs propres métabolites chez la levure Saccharomyces cerevisiae. I. Obtention et caractérisation de différentes classes de mutants. Genetics 56, 149–161.CrossRefGoogle ScholarPubMed
Meuris, P. (1969). Studies of mutants inhibited by their own metabolites in Saccharomyces cerevisiae. II. Genetic and enzymatic analysis of three classes of mutants. Genetics 63, 569–580.CrossRefGoogle ScholarPubMed
Pieniazek, N. J., Stepien, P. P. & Paszewski, A. (1973). An Aspergillus nidulans mutant lacking cystathionine β-synthase: Identity of L-serine sulfhydrylase with cystathionine β-synthase and its distinctness from O-acetyl-L-serine sulfhydrylase. Biochimica et Biophysica Acta 297, 37–47.CrossRefGoogle Scholar
Schlenk, F. & DePalma, R. B. (1957). The formation of S-adenosylmethionine in yeast. Journal of Biological Chemistry 229, 1037–1050.CrossRefGoogle ScholarPubMed
Smirnov, M. N., Smirnov, V. N., Budowsky, B. I., Inge-Vechtomov, S. G. & Serebrjakov, N. G. (1967). Red pigment of adenine-deficient yeast Saccharomyces cerebisiae. Biochemical and Biophysical Research Communications 27, 299–304.CrossRefGoogle Scholar
Strauss, A. (1972). Genetische und physiologische Untersuchungen an ad–6 – und ad-7- Mutanten von Schizosaccharomyces pombe. Diploma thesis, University of Bern.Google Scholar
Strauss, A. (1976).Isolierung und Klassifizierung von polyaromatisch auxotrophen Mutanten von Schizosaccharomyces pombe. Feinstrukturanalyse der multifunktionellen Genregion aro3. Ph.D. thesis, University of Bern.Google Scholar
Yanulaitis, A. A., Nikolaeva, Z. K. & Smirnov, M. N. (1975). Biochemical identification of mutations in gene ade2 of Saccharomyces cerevisiae. Biokhimiya 40, 588–591.Google Scholar