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Wild Arbutus unedo L. and Rubus ulmifolius Schott fruits are underutilized sources of valuable bioactive compounds with antioxidant capacity

Published online by Cambridge University Press:  17 October 2014

Brígida María Ruiz-Rodríguez ,
Concepción Sánchez-Moreno ,
Begoña De Ancos ,
María de Cortes Sánchez-Mata ,
Virginia Fernández-Ruiz ,
Montaña Cámara  and
Javier Tardío
Brígida María Ruiz-Rodríguez
Affiliation:
Fac. Farmacia, Univ. Complut. Madrid (UCM), Pza. Ramón y Cajal, s/n, ES-28040, Madrid, Spain
Concepción Sánchez-Moreno*
Affiliation:
Inst. Cienc. Tecnol. Aliment. Nutr., Cons. Super. Investig. Cient. (ICTAN-CSIC), José Antonio Novais 10, ES-28040 Madrid, Spain,. csanchezm@ictan.csic.es
Begoña De Ancos
Affiliation:
Inst. Cienc. Tecnol. Aliment. Nutr., Cons. Super. Investig. Cient. (ICTAN-CSIC), José Antonio Novais 10, ES-28040 Madrid, Spain,. csanchezm@ictan.csic.es
María de Cortes Sánchez-Mata
Affiliation:
Fac. Farmacia, Univ. Complut. Madrid (UCM), Pza. Ramón y Cajal, s/n, ES-28040, Madrid, Spain
Virginia Fernández-Ruiz
Affiliation:
Fac. Farmacia, Univ. Complut. Madrid (UCM), Pza. Ramón y Cajal, s/n, ES-28040, Madrid, Spain
Montaña Cámara
Affiliation:
Fac. Farmacia, Univ. Complut. Madrid (UCM), Pza. Ramón y Cajal, s/n, ES-28040, Madrid, Spain
Javier Tardío
Affiliation:
Inst. Madr. Investig. Desarro Rural, Agrar. Aliment. (IMIDRA), Finca “El Encín”, Apdo. 127, ES-28800, Alcalá de Henares, Madrid, Spain
*
* Correspondence and reprints
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Abstract

Introduction. Several studies reveal the important role played by ‘lesser-known’ wild fruits since they contain nutritional and functional compounds which have biological properties. Materials and methods. Our work studied the presence of bioactive compounds such as vitamin C (ascorbic acid and dehydroascorbic acid), total phenolic content, phenolic acids, flavonols, anthocyanins and the antioxidant capacity (FRAP, ABTS•+ and DPPH• in vitro tests) in wild fruits of Arbutus unedo L. and Rubus ulmifolius Schott of Spanish origin, including samples from different seasons and locations. Results and discussion. A wide variability was found in the composition of fruits of the same species, which substantiates the importance of analyzing several batches of wild fruits, to achieve representative results, taking into account the natural variability. Arbustus unedo fruits showed higher vitamin C, mainly in the ascorbic acid form, and phenolic content than R. ulmifolius [(172–419) mg ascorbic acid×100 g–1 fw vs. (5.99–26.83) mg ascorbic acid×100 g–1 fw, and (773–1621) mg total phenolic compounds×100 g–1 fw vs. (376–1326) mg total phenolic compounds×100 g–1 fw, respectively]; phenolic acids and anthocyanins are the major groups of phenolic compounds found in both species, with gallic acid and cyanidin 3-glucoside as the main compounds. Arbustus unedo fruits showed significantly higher Folin-Ciocalteu values than those of R. ulmifolius. These values are higher than those reported for the majority of berries. The significant correlations found among different antioxidant compounds (r > 0.6300, P < 0.001) may reveal a protective effect between ascorbic acid and phenolic acids or anthocyanins in the fruits. Therefore, Arbutus unedo and R. ulmifolius fruits should be considered as new important sources of safe antioxidants.

Keywords

SpainArbutus unedoRubus ulmifoliusfruitsphenolic compoundsphenolic contentantioxidantsEspagneArbutus unedoRubus ulmifoliusfruitscomposés phénoliquesteneur en phénolsantioxidantEspañaArbutus unedoRubus ulmifoliusfruitscompuestos fenólicoscontenido fenólicoantioxidantes
Type
Original article
Information
Fruits , Volume 69 , Issue 6 , November 2014 , pp. 435 - 448
Copyright
© 2014 Cirad/EDP Sciences

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References

Leonti, M.,Nebel, S.Rivera, D.,Heinrich, M., Wild gathered food plants in the European Mediterranean: a comparison analysis, Econ. Bot. 60 (2006) 130–142.CrossRefGoogle Scholar
Trichopoulou, A.,Vasilopoulou, E.,Hollman, P.,Chamalides, C.,Foufa, E.,Kaloudis, T.,Kromhout, D.,Miskaki, P.,Petrochilou, I.,Poulima, E.,Stafilakis, K.,Theophilou, D., Nutritional composition and flavonoid content of edible wild green pies: A potential rich source of antioxidant nutrients in the Mediterranean diet, Food Chem. 70 (2000) 319–323.CrossRefGoogle Scholar
Ruiz-Rodríguez, B.M., De Ancos, B.,Sánchez-Moreno, C.,Fernández-Ruiz, V.,Sánchez-Mata, M.C.,Cámara, M.,Tardío, J., Wild blackthorn (Prunus spinosa L.) and hawthorn (Crataegus monogyna Jacq.) fruits as valuable sources of antioxidants, Fruits 69 (2014) 61–73.CrossRefGoogle Scholar
Tardío, J., Pardo-de-Santayana, M., Morales, R., Ethnobotanical review of wild edible plants in Spain, Bot. J. Linn. Soc. 152 (2006) 27–71.CrossRefGoogle Scholar
Heinrich, M.,Nebel, S.,Leonti, M.,Rivera, D.,Obón, C., Local food-nutraceuticals: bridging the gap between local knowledge and global needs, Forum Nutr. 59 (2006) 1–17.Google ScholarPubMed
González-Tejero M.R., Investigaciones etnobotánicas en la provincia de Granada, Univ. Granada, Thesis, Spain, 1990.
Kivçak, B.,Mert, T.,Denizci, A.A., Antimicrobial activity of Arbutus unedo L., J. Pharm. Sci. 26 (2001) 125–128.Google Scholar
Ziyyat, A.,Mekhfi, H.,Bnouham, M.,Tahri, A.,Legssyer, A.,Hoerter, J.,Fischmeister, R., Arbutus unedo induces endothelium-dependent relaxation of the isolated rat aorta, Phytother. Res. 16 (2002) 572–575.CrossRefGoogle ScholarPubMed
Alarcão-E-Silva, M.L.C.M.M.,Leitão, A.E.B.,Azinheira, H.G.,Leitão, M.C.A., The arbutus berry: Studies on its color and chemical characteristics at two mature stages, J. Food Compos. Anal. 14 (2001) 27–35.CrossRefGoogle Scholar
Ganhao, R.,Morcuende, D.,Estevez, M., Protein oxidation in emulsified cooked burger patties with added fruit extracts: Influence on colour and texture deterioration during chill storage, Meat Sci. 85 (2010) 402–409. CrossRefGoogle ScholarPubMed
Pallauf, K.,Rivas-Gonzalo, J.C.,Castillo, M.D.,Cano, M.P.,Pascual-Teresa, S., Characterization of the antioxidant composition of strawberry tree (Arbutus unedo L.) fruits, J. Food Compos. Anal. 21 (2008) 273–281.CrossRefGoogle Scholar
Ruiz-Rodríguez, B.M.,Morales, P.,Fernández-Ruiz, V.,Sánchez-Mata, M.C.,Cámara, M.,Díez-Marqués, C., Pardo-de-Santayana, M., Molina, M., Tardío, J., Valorization of wild strawberry-tree fruits (Arbutus unedo L.) through nutritional assessment and natural production data, Food Res. Int. 44 (2011) 1244–1253.CrossRefGoogle Scholar
Greenfield H., Southgate, D.A.T., Food composition data: production, management and use, 2nd Ed., FAO, Rome, Italy, 2003.
Villar L., Arbutus, in: Castroviejo S., Aedo C., Gómez-Campo C., Laínz M., Montserrat P., Morales R., Muñoz-Garmendia F., Nieto-Feliner G., Rico E., Talavera S., Villar L. (Eds.), Flora Iberica, vol. IV, CSIC, Madrid, Spain, 1993, pp. 514–516.
Monasterio-Huelin E., Rubus, in: Muñoz-Garmendia F., Navarro C. (Eds.), Flora Iberica, vol VI., CSIC, Madrid, Spain, 1998, pp. 143–195.
Molina M., Producción y abundancia natural de verduras de hoja, esparragos y frutos carnosos silvestres de uso tradicional en España, Univ. Autón. Madrid, Thesis, Madrid, Spain, 2014.
Molina, M., Pardo-de-Santayana, M., Aceituno, L., Morales, R., Tardío, J., Fruit production of strawberry-tree ( Arbutus unedo L.) in two Spanish forests, Forestry 84 (2011) 419–429.CrossRefGoogle Scholar
Horwitz W., Latimer G.W., Official methods of analysis of AOAC international (18th ed.), Gaithersburg, EEUU, 2005.
Sánchez-Mata, M.C., Cabrera Loera, R.D., Morales, P., Fernández-Ruiz, V., Cámara, M., Díez-Marqués, C., Pardo-de-Santayana, M., Tardío, J., Wild vegetables of the Mediterranean area as valuable sources of bioactive compounds, Genet. Resour. Crop Evol. 59 (2012) 431–443.CrossRefGoogle Scholar
Pérez-Jiménez, J.,Arranz, S.,Saura-Calixto, F., Proanthocyanidin content in foods is largely underestimated in the literature data: An approach to quantification of the missing proanthocyanidins, Food Res. Int. 42 (2006) 1381–1388.CrossRefGoogle Scholar
Huang, D.,Ou, B.,Prior, R.L., The chemistry behind antioxidant capacity assays, J. Agric. Food Chem. 53 (2005) 1841–1856.CrossRefGoogle ScholarPubMed
Magalhães, L.M.,Santos, F.,Segundo, M.A.,Reis, S.,Lima, J.L.F.C., Rapid microplate high-throughput methodology for assessment of Folin-Ciocalteu reducing capacity, Talanta 83 (2010) 441–447.CrossRefGoogle ScholarPubMed
Singleton, V.L.,Rossi, J.A., Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents, Am. J. Enol. Vitic. 16 (1965) 144–158.Google Scholar
Benzie, I.F.F.,Strain, J.J., The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay, Anal. Biochem. 239 (1) (1996) 70–76.CrossRefGoogle ScholarPubMed
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med., 26 (1999) 1231–1237.CrossRefGoogle ScholarPubMed
Sánchez-Moreno, C.,Larrauri, J.A.,Saura-Calixto, F., A procedure to measure the antiradical efficiency of polyphenols, J. Sci. Food Agric. 76 (1998) 270–276.3.0.CO;2-9>CrossRefGoogle Scholar
Fang, F.,Li, J.-M.,Zhang, P.,Tang, K.,Wang, W.,Pan, Q.-H.,Huang, W.-D., Effects of grape variety, harvest date, fermentation vessel and wine ageing on flavonoid concentration in red wines, Food Res. Int. 41 (2008) 53–60.CrossRefGoogle Scholar
Ercisli, S., Chemical composition of fruits in some rose (Rosa spp.) species, Food Chem. 104 (2007) 1379–1384.CrossRefGoogle Scholar
Barros, L.,Carvalho, A.M.,Morais, J.S.,Ferreira, I.C.F.R., Strawberry-tree, blackthorn and rose fruits: Detailed characterisation in nutrients and phytochemicals with antioxidant properties, Food Chem. 120 (2010) 247–254.CrossRefGoogle Scholar
Marinova, D.,Ribarova, F.,Atanassova, M., Total phenolics and total flavonoids in Bulgarian fruits and vegetables, J. Univ. Chem. Technol. Metall. 40 (2005) 255–260.Google Scholar
Pawlowska, A.G., De Leo, M.,Braca, A., Phenolics of Arbutus unedo L. (Ericaceae) fruits: Identification of anthocyanins and gallic acid derivatives, J. Agric. Food. Chem. 54 (2006) 10234–10238.CrossRefGoogle ScholarPubMed
Peñuelas, J.,Sardans, J.,Ogaya, R.,Estiarte, M., Nutrient stoichiometric relations and biogeochemical niche in coexisting plant species: Effect of simulated climate change, Pol. J. Ecol. 56 (2008) 613–622.Google Scholar
Souci S.W., Fachmann W., Kraut H., Food composition and nutrition tables, 7th ed., Medpharm Scient. Publ., Stuttgart, Ger., 2008.
Gordon, A.,Friedrich, M.,Matta, V.M., da Moura, C.F.H.,Marx, F., Changes in phenolic composition, ascorbic acid and antioxidant capacity in cashew apple (Anacardium occidentale L.) during ripening, Fruits 67 (2012) 267–276.CrossRefGoogle Scholar
Lal, S.,Ahmed, N.,Singh, S.R.,Singh, D.B.,Sharma, O.C.,Kumar, R., Variability of health and bioactive compounds in strawberry (Fragaria × ananassa Duch.) cultivars grown under an Indian temperate ecosystem, Fruits 68 (2013) 423–434.CrossRefGoogle Scholar
Sellappan, S.,Akoh, C.C.,Krewer, G., Phenolic compounds and antioxidant capacity of Georgia-grown blueberries and blackberries, J. Agric. Food. Chem. 50 (2002) 2432–2438.CrossRefGoogle ScholarPubMed
Česonienė, L.,Jasutienė, I.,Šarkinas, A., Phenolics and anthocyanins in berries of European cranberry and their antimicrobial activity, Medicina (Kaunas) 45 (2009) 992–999.Google ScholarPubMed
Määttä-Riihinen, K.R.,Kamal-Eldin, A.,Mattila, P.H.,González-Paramás, A.M.,Törrönen, A.R., Distribution and contents of phenolic compounds in eighteen Scandinavian berry species, J. Agric. Food. Chem. 52 (2004) 4477–4486.CrossRefGoogle ScholarPubMed
Kaume, L.,Howard, L.R.,Devareddy, L., The blackberry fruit: A review on its composition and chemistry, metabolism and bioavailability, and health benefits, J. Agric. Food. Chem. 60 (2012) 5716–5727.CrossRefGoogle ScholarPubMed
Egea, I.,Sánchez-Bel, P.,Romojaro, F.,Pretel, M.T., Six edible wild fruits as potential antioxidant additives or nutritional supplements, Plant Foods Hum. Nutr. 65 (2010) 121–129.CrossRefGoogle ScholarPubMed