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Radio-sensitivity of in vivo and in vitro cultures of banana cv. Basrai (AAA)

  • Vidyadhar Mukund Karmarkar (a1), Vishvas Manohar Kulkarni (a2), Penna Suprasanna (a2), Vishwas Anand Bapat (a2) and Pavagada Sanjeeva Rao (a2)...

Abstract

Introduction. Banana is a worldwide important fruit crop, and improved clones which are resistant/tolerant to different biotic and abiotic stresses are very much required. In this regard, prospects of in vitro mutagenesis are high and relevant. Materials and methods. The in vitro cultures (individual and multiple shoots) and in vivo plant materials (suckers and hardened plants) of banana cv. Basrai (AAA) were exposed to 60Co gamma ray doses ranging from 0-100 Gy. Radio-sensitivity of suckers and in vitro individual shoots was assessed by recording data on survival, multiplication ratio, days to root initiation, root number, root length, shoot length, plant height, number of leaves, leaf area and chlorophyll content. Results. Increase in the dose of gamma-rays resulted in corresponding decrease in the growth of the explants. Further, as a trend, the in vivo explants were observed to be more vulnerable than the in vitro ones. This offers possibilities of obtaining live plant materials even at high doses thereby increasing the probability of higher frequency of mutations. Lower doses of 10 and 20 Gy had an enhancing effect on the multiplication ratio of in vitro multiple shoot cultures. Conclusion. The in vivo and in vitro plant materials exhibited differential response to gamma-irradiation. The results obtained from the present studies will be useful in refining strategies for in vitro mutation induction aimed at banana improvement.

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Keywords

Radio-sensitivity of in vivo and in vitro cultures of banana cv. Basrai (AAA)

  • Vidyadhar Mukund Karmarkar (a1), Vishvas Manohar Kulkarni (a2), Penna Suprasanna (a2), Vishwas Anand Bapat (a2) and Pavagada Sanjeeva Rao (a2)...

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