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Integrated genetic map of citrus based on RAPD markers

Published online by Cambridge University Press:  15 September 2005

Roberto Pedroso de Oliveira ,
Mariângela Cristofani  and
Marcos Antonio Machado
Roberto Pedroso de Oliveira
Affiliation:
Embrapa-Centro de Pesquisa Agropecuária de Clima Temperado (CPACT), PO Box 403, 96001-970, Pelotas-RS, Brazil
Mariângela Cristofani
Affiliation:
Centro APTA Citros ‘Sylvio Moreira’ / IAC, PO Box 04, 13490-970, Cordeirópolis-SP, Brazil
Marcos Antonio Machado
Affiliation:
Centro APTA Citros ‘Sylvio Moreira’ / IAC, PO Box 04, 13490-970, Cordeirópolis-SP, Brazil
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Abstract

Introduction. The citrus industry is one of the main activities of Brazilian agriculture. Several pests and diseases have threatened citrus culture in recent years. Genetic mapping is one of the most efficient strategies for conducting advanced genetic studies by facilitating plant selection guided by markers. The objective of this study was to construct an integrated genetic map between Citrus sinensis (L.) Osbeck cv. ‘Pêra’ and C. reticulata Blanco cv. ‘Cravo’ using two different types of segregation of markers. Materials and methods. The linkage analyses were conducted with segregation data obtained from 256 RAPD markers in a population of 94 hybrids. Analyses were performed through the software JoinMap, LOD ≥ 6.0, θ≤ 0.25, and the Kosambi function. Results and discussion. The linked map between the two cultivars had 217 markers defined by 15 linkage groups, covering 527 cM. This map had five linkage groups with common markers of ‘Pêra’ and ‘Cravo’. The inclusion of new markers would increase the number of linkage groups with markers from both parents, thus making their number equal to the haploid chromosome number. The alterations in the order and in the distances among the markers, due to the presence or absence of bridging markers in the linkage map, are discussed.

Keywords

Citrus reticulataCitrus sinensisgenetic mapsgenetic markersRAPDCitrus reticulataCitrus sinensiscarte génétiquemarqueur génétiqueRAPD
Type
Research Article
Information
Fruits , Volume 60 , Issue 3 , May 2005 , pp. 187 - 193
Copyright
© CIRAD, EDP Sciences

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References

Anon., Mapeamento da fruticultura brasileira, Minist. Agric. do Abast., Brasilia, Brasil, 2000.
Wu, R.L., Zeng, Z.B., McKeand, S.E., O’Malley D.M., The case for molecular mapping in forest tree breeding, Plant Breed. Rev. 19 (2000) 41–68.
Grosser, J.W., Gmitter, F.G. Jr., Somatic hybridization of Citrus with wild relatives for germplasm enhancement and cultivar development, HortScience 25 (1990) 147–151.
Durham, R.E., Liou, P.C., Gmitter, F.G. Jr., Moore, G.A., Linkage of restriction fragment length polymorphisms and isozymes in Citrus, Theor. Appl. Genet. 84 (1992) 39–48. CrossRef
Roose, M.L., Fang, D., Cheng, F.S., Tayyar, R.I, Federici, C.T., Kupper, R.S., Mapping the Citrus genome, Acta Hortic. 535 (2000) 25–32. CrossRef
Gmitter, F.G. Jr., Xiao, S.Y., Huang, S., Hu, X.L., Garnsey, S.M., Deng, Z., A localized linkage map of the virus tristeza virus resistance gene region, Theor. Appl. Genet. 92 (1996) 688–695. CrossRef
Guerra, M.S., Cytogenetics of Rutaceae. II. Nuclear DNA content, Caryologia 37 (1984) 219–226. CrossRef
Moore, G.A., Tozlu, I., Weber, C.A., Guy, C.L., Mapping quantitative trait loci for salt tolerance and cold tolerance in Citrus grandis (L.) Osb. × Poncirus trifoliata (L.) Raf. hybrid populations, Acta Hortic. 535 (2000) 37–45. CrossRef
Cristofani, M., Machado, M.A., Grattapaglia, D., Genetic linkage maps of Citrus sunki Hort. ex. Tan. and Poncirus trifoliata (L.) Raf. and mapping of citrus tristeza virus resistance gene, Euphytica 109 (1999) 25–32. CrossRef
Roose, M.L., Jarrell, D.C., Kupper, R.S., Genetic mapping in a Citrus × Poncirus F2 population, Proc. Int. Soc. Citriculture 7 (1992) 210–213.
Van Ooijen J.W., Voorrips R.E., JoinMap® Version 3.0, software for the calculation of genetic linkage maps (software), Plant Res. Int., Wageningen, Nederland, 2001.
Jacobs, J.M.E., Van Eck, H.J., Arens, P., Verkerk-Bakker, B., Lintel-Hekkert, B., Bastiaansen, H.J.M., El-Kharbotly, A., Pereira, A., Jacobsen, E., Stiekema, W.J., A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons and classical markers, Theor. Appl. Genet. 91 (1995) 289–300. CrossRef
Qi, X., Stam, P., Lindhout, P., Comparison and integration of four barley genetic maps, Theor. Appl. Genet. 93 (1996) 234–241.
Sebastian, R.L., Howell, E.C., King, G.J., Marshall, D.F, Kearsey, M.J., An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations, Theor. Appl. Genet. 100 (2000) 75–81. CrossRef
Oliveira, R.P., Novelli, V.M., Machado, M.A., Freqüência de híbridos em cruzamento entre tangerina ‘Cravo’ e laranja ‘Pêra’: análise de marcadores morfológicos e RAPD, Pesqui. Agropecu. Brasil. 35 (2000) 1895–1903. CrossRef
Oliveira, R.P., Cristofani, M., Aguilar-Vildoso, C.I., Machado, M.A., Diversidade genética entre híbridos de tangerina ‘Cravo’ e laranja ‘Pêra’ utilizando marcadores RAPD, Pesqui. Agropecu. Brasil. 37 (2002) 479–484. CrossRef
Kosambi, D.D., The estimation of map distance from recombination values, Ann. Eugenet. 12 (1944) 172–175. CrossRef
Davies F.S., Albrigo L.G., Citrus, CAB Int., Wallingford, UK, 1994.
Federici, C.T., Fang, D.Q., Scora, R.W., Roose, M.L., Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analyses, Theor. Appl. Genet. 96 (1998) 812–822. CrossRef
Oliveira R.P., Mapping of ‘Pêra’ sweet orange (Citrus sinensis L. Osbeck) and ‘Cravo’ mandarin (C. reticulata Blanco) by pseudo-testcross, Univ. São Paulo, Thesis, Piracicaba, Brazil, 2001, 181 p.
Hemmat, M., Weeden, N.F. Manganaris, A.G., Lawson, D.M., Molecular marker linkage map for apple, J. Hered. 85 (1994) 4–11.
Grattapaglia, D., Sederoff, R., Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers, Genetics 137 (1994) 1121–1137.
Nienhuis, J., Helentjaris, T., Slocum, M., Ruggero, B., Schaefer, A., Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato, Crop Sci. 27 (1987) 797–803. CrossRef
Tanksley, S.D., Ganal, M.W., Prince, J.P., De Vicente, M.C., Bonierbale, M.W., Broun, P., Fulton, T.M., Giovannoni, J.J., Grandillo, S., Martin, G.B., Messeguer, R., Miller, J.C., Miller, L., Paterson, A.H., Pineda, O., Roder, M.S., Wing, R.A., Wu, W., Young, N.D., High-density molecular linkage maps of the tomato and potato genomes, Genetics 132 (1992) 1141–1160.
Cai, Q., Guy, C.L., Moore, G.A., Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci, Theor. Appl. Genet. 89 (1994) 606–614. CrossRef