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Tracing functionally identified neurones in a multisynaptic pathway in the hamster and rat using herpes simplex virus expressing green fluorescent protein

Published online by Cambridge University Press:  19 November 2001

S. Pyner ,
J. Cleary ,
P. McLeish ,
A. Buchan  and
J. H. Coote
S. Pyner
Affiliation:
Division of Medical Sciences - Physiology and Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
J. Cleary
Affiliation:
Division of Medical Sciences - Physiology and Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
P. McLeish
Affiliation:
Division of Medical Sciences - Physiology and Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
A. Buchan
Affiliation:
Division of Medical Sciences - Physiology and Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
J. H. Coote
Affiliation:
Division of Medical Sciences - Physiology and Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
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Abstract

Using a genetically modified herpes simplex virus encoding green fluorescent protein we sought to establish if this viral modification could be used in transneuronal tracing studies of the sympathetic nervous system. The herpes simplex virus encoding green fluorescent protein was injected into the adrenal medulla of three hamsters and six rats. After a suitable survival period, neurones in the sympathetic intermediolateral cell column of the thoracolumbar spinal cord, rostral ventral medulla and paraventricular nucleus of the hypothalamus were clearly identified by the presence of a green fluorescence in the cytoplasm of the neurones of both species. Thus, herpes simplex virus encoding green fluorescent protein labelled chains of sympathetic neurones in the hamster and rat and therefore has the potential to be used in transneuronal tracing studies of autonomic pathways in these species. Experimental Physiology (2001) 86.6, 695-702.

Type
Full Length Papers
Information
Experimental Physiology , Volume 86 , Issue 6 , November 2001 , pp. 695 - 702
Copyright
© The Physiological Society 2001

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