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Effect of postnatal age and a β3-adrenergic agonist (Zeneca D7114) administration on uncoupling protein-1 abundance in the lamb

  • J. A. Bird (a1), A. Mostyn (a1), L. Clarke (a1), D. T. Juniper (a1), H. Budge (a1), T. Stephenson (a1) and M. E. Symonds (a1)...

Abstract

We examined the effect of time after birth and β3-adrenergic agonist (Zeneca D7114) administration on uncoupling protein-1 (UCP1) abundance and thermoregulation in the lamb. Forty twin lambs, all born normally at term, were maintained at a cold ambient temperature of between 3 and 8 °C. At 0.5, 1.75, 5.25, 11.25 and 23.25 h after birth eight sets of twins were fed 20 ml of formula milk ± 10 mg kg-1 of β3-adrenergic agonist, and 45 min after feeding brown adipose tissue (BAT) was sampled. Colonic temperature was measured and BAT analysed for UCP1 abundance, GDP-binding to mitochondrial protein (i.e. thermogenic activity) and catecholamine content. Colonic temperature declined between 1.25 and 6 h from 40.2 °C to 39.2 °C and then increased to 39.8 °C at 12 h, but increased after feeding at all ages. UCP1 abundance increased from 1.25 h after birth, to peak at 2 h after birth in controls, compared with 6 h after birth in β3-adrenergic agonist-treated lambs. The level of GDP-binding to mitochondrial protein did not change significantly with age but was increased by β3-adrenergic agonist treatment. The noradrenaline (norepinephrine) content of BAT increased between 1.25 and 12 h after birth, irrespective of β3-adrenergic agonist administration. The total weight of perirenal BAT plus its lipid, protein and mitochondrial protein content declined over the first 6 h of life. UCP1 development continues over the first 24 h of neonatal life, and can be manipulated by β3-adrenergic agonist administration. This may represent one method of improving thermoregulation in newborn lambs. Experimental Physiology (2001) 86.1, 65-70.

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Effect of postnatal age and a β3-adrenergic agonist (Zeneca D7114) administration on uncoupling protein-1 abundance in the lamb

  • J. A. Bird (a1), A. Mostyn (a1), L. Clarke (a1), D. T. Juniper (a1), H. Budge (a1), T. Stephenson (a1) and M. E. Symonds (a1)...

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